Literature summary for 1.12.1.4 extracted from
Maso, L.; Galazzo, L.; Vallese, F.; Di Valentin, M.; Albertini, M.; De Rosa, E.; Giacometti, G.; Costantini, P.; Carbonera, D.
A conformational study of the GTPase domain of [FeFe]-hydrogenase maturation protein HydF by PELDOR spectroscopy (2015), Appl. Magn. Reson., 46, 465-479 .
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
expressed in Escherichia coli Rosetta (DE3) cells |
Thermotoga neapolitana |
Organism
Organism |
UniProt |
Comment |
Textmining |
Thermotoga neapolitana |
- |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
nickel affinity column chromatography, and Superose 12 gel filtration |
Thermotoga neapolitana |
Subunits
Subunits |
Comment |
Organism |
? |
x * 25000, subunit HydF, SDS-PAGE |
Thermotoga neapolitana |
Synonyms
Synonyms |
Comment |
Organism |
HydF |
- |
Thermotoga neapolitana |
[FeFe]-hydrogenase |
- |
Thermotoga neapolitana |
Cofactor
Cofactor |
Comment |
Organism |
Structure |
[4Fe-4S]-center |
- |
Thermotoga neapolitana |
|
General Information
General Information |
Comment |
Organism |
physiological function |
an active HydF GTPase domain is essential to produce a functional [FeFe]-hydrogenase |
Thermotoga neapolitana |