Reference to 1.12.1.4; Id = 716106 - BRENDA Enzyme Database

Fe2+

the holoprotein contains 31.1 mol iron per mol enzyme

Thermotoga maritima

18025

-

1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence

Thermotoga maritima

19000

-

1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE

Thermotoga maritima

68000

-

1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE

Thermotoga maritima

68676

-

1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence

Thermotoga maritima

72248

-

1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence

Thermotoga maritima

73000

-

1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE

Thermotoga maritima

158900

-

gel filtration

Thermotoga maritima

160000

-

calculated from amino acid sequence

Thermotoga maritima

NADH + H+ + reduced ferredoxin

Thermotoga maritima

-

H2 + NAD+ + oxidized ferredoxin

-

?

NADH + H+ + reduced ferredoxin

Thermotoga maritima DSM 3109

-

H2 + NAD+ + oxidized ferredoxin

-

?

Thermotoga maritima

-

Thermotoga maritima DSM 3109

-

DEAE-Sepharose column chromatography, Q-Sepharose column chromatography, hydroxyapatite column chromatography, phenyl-Sepharose column chromatography, and Superdex 200 gel filtration

Thermotoga maritima

NADH + H+ + reduced ferredoxin

-

716106

Thermotoga maritima

H2 + NAD+ + oxidized ferredoxin

-

?

NADH + H+ + reduced ferredoxin

-

716106

Thermotoga maritima DSM 3109

H2 + NAD+ + oxidized ferredoxin

-

?

sodium dithionite + H+ + reduced anthraquinone-2,6-disulfonic acid

-

716106

Thermotoga maritima

H2 + oxidized anthraquinone-2,6-disulfonic acid + ?

-

?

sodium dithionite + H+ + reduced anthraquinone-2,6-disulfonic acid

-

716106

Thermotoga maritima DSM 3109

H2 + oxidized anthraquinone-2,6-disulfonic acid + ?

-

?

sodium dithionite + H+ + reduced methyl viologen

-

716106

Thermotoga maritima

H2 + oxidized methyl viologen + ?

-

?

sodium dithionite + H+ + reduced methyl viologen

-

716106

Thermotoga maritima DSM 3109

H2 + oxidized methyl viologen + ?

-

?

heterotrimer

1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence

Thermotoga maritima

heterotrimer

1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE

Thermotoga maritima

Fe-only hydrogenase

-

Thermotoga maritima

flavin

-

Thermotoga maritima

iron-sulfur centre

the alpha subunit contains two [2Fe-2S] clusters and three [4Fe-4S] clusters, the beta subunit contains three [4Fe-4S] clusters and one [2Fe-2S] clusters, the gamma subunit contains one [2Fe-2S] cluster

Thermotoga maritima

flavin

-

Thermotoga maritima

iron-sulfur centre

the alpha subunit contains two [2Fe-2S] clusters and three [4Fe-4S] clusters, the beta subunit contains three [4Fe-4S] clusters and one [2Fe-2S] clusters, the gamma subunit contains one [2Fe-2S] cluster

Thermotoga maritima

Fe2+

the holoprotein contains 31.1 mol iron per mol enzyme

Thermotoga maritima

18025

-

1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence

Thermotoga maritima

19000

-

1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE

Thermotoga maritima

68000

-

1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE

Thermotoga maritima

68676

-

1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence

Thermotoga maritima

72248

-

1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence

Thermotoga maritima

73000

-

1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE

Thermotoga maritima

158900

-

gel filtration

Thermotoga maritima

160000

-

calculated from amino acid sequence

Thermotoga maritima

NADH + H+ + reduced ferredoxin

Thermotoga maritima

-

H2 + NAD+ + oxidized ferredoxin

-

?

NADH + H+ + reduced ferredoxin

Thermotoga maritima DSM 3109

-

H2 + NAD+ + oxidized ferredoxin

-

?

DEAE-Sepharose column chromatography, Q-Sepharose column chromatography, hydroxyapatite column chromatography, phenyl-Sepharose column chromatography, and Superdex 200 gel filtration

Thermotoga maritima

NADH + H+ + reduced ferredoxin

-

716106

Thermotoga maritima

H2 + NAD+ + oxidized ferredoxin

-

?

NADH + H+ + reduced ferredoxin

-

716106

Thermotoga maritima DSM 3109

H2 + NAD+ + oxidized ferredoxin

-

?

sodium dithionite + H+ + reduced anthraquinone-2,6-disulfonic acid

-

716106

Thermotoga maritima

H2 + oxidized anthraquinone-2,6-disulfonic acid + ?

-

?

sodium dithionite + H+ + reduced anthraquinone-2,6-disulfonic acid

-

716106

Thermotoga maritima DSM 3109

H2 + oxidized anthraquinone-2,6-disulfonic acid + ?

-

?

sodium dithionite + H+ + reduced methyl viologen

-

716106

Thermotoga maritima

H2 + oxidized methyl viologen + ?

-

?

sodium dithionite + H+ + reduced methyl viologen

-

716106

Thermotoga maritima DSM 3109

H2 + oxidized methyl viologen + ?

-

?

heterotrimer

1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence

Thermotoga maritima

heterotrimer

1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE

Thermotoga maritima

741931

Birrell

Importance of hydrogen bondin ...

Thermotoga maritima, Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589

Biochemistry

55

4344-4355

2016

-

1

-

2

-

2

-

24

-

1

-

2

-

2

-

1

-

1

-

2

-

2

-

1

-

2

-

741708

Maso

-

A conformational study of the ...

Thermotoga neapolitana

Appl. Magn. Reson.

46

465-479

2015

-

1

-

1

-

1

-

1

2

-

1

-

1

-

1

-

1

-

1

-

740625

Zheng

Hydrogen formation and its reg ...

Ruminococcus albus, Ruminococcus albus DSM 20455

J. Bacteriol.

196

3840-3852

2014

-

3

-

2

-

1

-

1

-

2

1

-

3

-

3

-

3

-

1

-

1

-

2

1

-

742697

Albertini

-

The proton iron-sulfur cluste ...

Thermotoga neapolitana

Int. J. Hydrogen Energy

39

18574-18582

2014

-

1

-

1

-

1

-

1

2

-

1

-

1

-

1

-

1

-

740618

Wang

A reversible electron-bifurcat ...

Moorella thermoacetica

J. Bacteriol.

195

1267-1275

2013

-

2

-

1

4

-

1

-

1

-

3

-

4

1

2

-

4

-

4

-

2

-

1

4

-

1

-

3

-

4

1

-

724434

Berto

The [4Fe-4S]-cluster coordinat ...

Thermotoga neapolitana

Biochim. Biophys. Acta

1817

2149-2157

2012

-

1

-

5

-

1

-

1

-

1

-

1

-

5

-

712281

Schut

The iron-hydrogenase of Thermo ...

Thermotoga maritima, Thermotoga maritima DSM 3109

J. Bacteriol.

191

4451-4457

2009

-

1

-

2

-

16

-

3

1

3

-

2

-

2

-

1

-

2

-

3

1

-

716106

Verhagen

Fe-only hydrogenase from Therm ...

Thermotoga maritima, Thermotoga maritima DSM 3109

Methods Enzymol.

331

216-226

2001

-

1

8

2

-

11

-

1

-

6

2

1

-

2

-

2

-

1

8

2

-

1

-

6

2

-

Fe-only hydrogenase from Thermotoga maritima

Data extracted from this reference: