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Literature summary for 1.12.1.3 extracted from

  • Germer, F.; Zebger, I.; Saggu, M.; Lendzian, F.; Schulz, R.; Appel, J.
    Overexpression, isolation and spectroscopic characterization of the bidirectional [NiFe]-hydrogenase from Synechocystis sp. PCC 6803 (2009), J. Biol. Chem., 284, 36462-36472.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.12.1.3

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Synechocystis Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2 + NADP+ Synechocystis sp.
-
 H+ + NADPH
-
 ?

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
single affinity chromatography, 4°C, aerobic conditions Synechocystis sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.076
-
 crude extract Synechocystis sp.
87.78
-
 after affinity chromatogrphy Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + NADP+
-
 Synechocystis sp. H+ + NADPH
-
 ?

Subunits

Subunits Comment Organism
pentamer 16.5% tricine-SDS Synechocystis sp.

Synonyms

Synonyms Comment Organism
NiFe-hydrogenase
-
 Synechocystis sp.