Literature summary for 1.11.2.5 extracted from

  • Tang, M.C.; Fu, C.Y.; Tang, G.L.
    Characterization of SfmD as a heme peroxidase that catalyzes the regioselective hydroxylation of 3-methyltyrosine to 3-hydroxy-5-methyltyrosine in saframycin A biosynthesis (2012), J. Biol. Chem., 287, 5112-5121.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
C-terminal His8-tagged SfmD is overexpressed in Escherichia coli BL21 (DE3) Streptomyces lavendulae

Protein Variants

Protein Variants Comment Organism
H274A mutant loses ability to hydroxylate 3-methyl-L-tyrosine Streptomyces lavendulae
H191A mutant loses ability to hydroxylate 3-methyl-L-tyrosine Streptomyces lavendulae
H313A mutant loses ability to hydroxylate 3-methyl-L-tyrosine. Heme content of the mutant enzyme is very low, very little heme-Fe binding ability Streptomyces lavendulae
H317A mutant loses ability to hydroxylate 3-methyl-L-tyrosine. Heme content of the mutant enzyme is very low, very little heme-Fe binding ability Streptomyces lavendulae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
L-tyrosine pH 9.0, 25°C Streptomyces lavendulae
0.63
-
3-methyl-L-tyrosine pH 9.0, 25°C Streptomyces lavendulae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39800
-
x * 39800, SDS-PAGE Streptomyces lavendulae
39854
-
x * 39854, calculated from sequence (without cofactor) Streptomyces lavendulae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-methyl-L-tyrosine + H2O2 + H+ Streptomyces lavendulae the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family 3-hydroxy-5-methyl-L-tyrosine + H2O
-
?
3-methyl-L-tyrosine + H2O2 + H+ Streptomyces lavendulae NRRL 11002 the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family 3-hydroxy-5-methyl-L-tyrosine + H2O
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces lavendulae B0CN28
-
-
Streptomyces lavendulae NRRL 11002 B0CN28
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces lavendulae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methyl-L-tyrosine + H2O2 + H+ the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family Streptomyces lavendulae 3-hydroxy-5-methyl-L-tyrosine + H2O
-
?
3-methyl-L-tyrosine + H2O2 + H+ the enzyme catalyzes the regioselective hydroxylation of 3-methyltyrosine to 3-hydroxy-5-methyl-L-tyrosine Streptomyces lavendulae 3-hydroxy-5-methyl-L-tyrosine + H2O
-
?
3-methyl-L-tyrosine + H2O2 + H+ the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family Streptomyces lavendulae NRRL 11002 3-hydroxy-5-methyl-L-tyrosine + H2O
-
?
3-methyl-L-tyrosine + H2O2 + H+ the enzyme catalyzes the regioselective hydroxylation of 3-methyltyrosine to 3-hydroxy-5-methyl-L-tyrosine Streptomyces lavendulae NRRL 11002 3-hydroxy-5-methyl-L-tyrosine + H2O
-
?
L-tyrosine + H2O2 + H+
-
Streptomyces lavendulae ?
-
?
L-tyrosine + H2O2 + H+
-
Streptomyces lavendulae NRRL 11002 ?
-
?

Subunits

Subunits Comment Organism
? x * 39800, SDS-PAGE Streptomyces lavendulae
? x * 39854, calculated from sequence (without cofactor) Streptomyces lavendulae

Synonyms

Synonyms Comment Organism
SacD
-
Streptomyces lavendulae
SfmD
-
Streptomyces lavendulae
3-methyltyrosine peroxidase
-
Streptomyces lavendulae
3-methyl-L-tyrosine peroxidase
-
Streptomyces lavendulae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Streptomyces lavendulae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.205
-
L-tyrosine pH 9.0, 25°C Streptomyces lavendulae
0.476
-
3-methyl-L-tyrosine pH 9.0, 25°C Streptomyces lavendulae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Streptomyces lavendulae

Cofactor

Cofactor Comment Organism Structure
heme a heme containing protein. The conserved motif HXXXC is crucial for heme binding. SfmD binds one molecular of heme Streptomyces lavendulae

General Information

General Information Comment Organism
physiological function the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family Streptomyces lavendulae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.76
-
3-methyl-L-tyrosine pH 9.0, 25°C Streptomyces lavendulae
0.205
-
L-tyrosine pH 9.0, 25°C Streptomyces lavendulae