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Literature summary for 1.11.2.1 extracted from

  • Rotilio, L.; Swoboda, A.; Ebner, K.; Rinnofner, C.; Glieder, A.; Kroutil, W.; Mattevi, A.
    Structural and biochemical studies enlighten the unspecific peroxygenase from Hypoxylon sp. EC38 as an efficient oxidative biocatalyst (2021), ACS Catal., 11, 11511-11525 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.2.1

Activating Compound

Activating Compound Comment Organism Structure
acetone 2% (v/v) acetone boosts the activity of the enzyme by up to 2fold Hypoxylon sp. EC38
acetonitrile 5% (v/v) acetonitrile boosts the activity of the enzyme by up to 6fold Hypoxylon sp. EC38

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Hypoxylon sp. EC38

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method Hypoxylon sp. EC38

Inhibitors

Inhibitors Comment Organism Structure
DMSO DMSO is hardly tolerated by the enzyme (the residual activity drops to near 0% with 2% (v/v) DMSO) Hypoxylon sp. EC38
H2O2 the enzyme retains 75% and 40% of the activity in the presence of 5 mM and 10 mM H2O2 whereas the optimal activity is observed at 2 mM H2O2 Hypoxylon sp. EC38

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
 5-nitro-1,3-benzodioxole at pH 7.0 and 25°C Hypoxylon sp. EC38
0.03
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) at pH 4.5 and 25°C Hypoxylon sp. EC38
0.054
-
 indole at pH 7.0 and 25°C Hypoxylon sp. EC38
0.0575
-
 2,6-dimethoxyphenol at pH 5.0 and 25°C Hypoxylon sp. EC38
0.68
-
 benzyl alcohol at pH 7.0 and 25°C Hypoxylon sp. EC38
0.758
-
 3,4-dimethoxybenzyl alcohol at pH 4.0 and 25°C Hypoxylon sp. EC38

Organism

Organism UniProt Comment Textmining
Hypoxylon sp. EC38 A0A1Y2TH07
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Hypoxylon sp. EC38

Purification (Commentary)

Purification (Comment) Organism
CaptoQ column chromatography and His-Trap column chromatography Hypoxylon sp. EC38

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-chloro-4-ethenylbenzene + H2O2
-
 Hypoxylon sp. EC38 (2R)-2-(4-chlorophenyl)oxirane + H2O
-
 ?
1-phenylethanol + H2O2
-
 Hypoxylon sp. EC38 1-phenylethanone + H2O
-
 ?
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Hypoxylon sp. EC38 oxidized 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?
2,6-dimethoxyphenol + H2O2
-
 Hypoxylon sp. EC38 ?
-
 ?
3,4-dimethoxybenzyl alcohol + H2O2
-
 Hypoxylon sp. EC38 3,4-dimethoxybenzaldehyde + H2O
-
 ?
5-nitro-1,3-benzodioxole + H2O2
-
 Hypoxylon sp. EC38 4-nitrocatechol + formic acid + H2O
-
 ?
benzyl alcohol + H2O2
-
 Hypoxylon sp. EC38 benzaldehyde + H2O
-
 ?
cyclohexene + H2O2
-
 Hypoxylon sp. EC38 cyclohexene oxide + H2O
-
 ?
ethenylbenzene + H2O2
-
 Hypoxylon sp. EC38 (2S)-2-phenyloxirane + H2O
-
 ?
ethylbenzene + H2O2 39% conversion Hypoxylon sp. EC38 (1R)-1-phenylethanol + acetophenone + H2O
-
 ?
indole + H2O2
-
 Hypoxylon sp. EC38 ?
-
 ?
indole + H2O2
-
 Hypoxylon sp. EC38 indigo + H2O
-
 ?
naphthalene + H2O2
-
 Hypoxylon sp. EC38 ?
-
 ?
veratryl alcohol + H2O2
-
 Hypoxylon sp. EC38 3,4-dimethoxybenzaldehyde + 3,4-dimethoxybenzoic acid + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Hypoxylon sp. EC38

Synonyms

Synonyms Comment Organism
UPO
-
 Hypoxylon sp. EC38

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35 50 at 35°C, there is some activity loss after 60 min but the enzyme remains mostly active even after longer incubations. The enzyme is mostly inactivated after 20-30 minute exposures to temperatures higher than 50°C. Conversely, the enzyme overall activity is minimally affected at 40°C and after 90 min the enzyme mostly loses activity Hypoxylon sp. EC38

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.89
-
 indole at pH 7.0 and 25°C Hypoxylon sp. EC38
4.56
-
 benzyl alcohol at pH 7.0 and 25°C Hypoxylon sp. EC38
7.15
-
 3,4-dimethoxybenzyl alcohol at pH 4.0 and 25°C Hypoxylon sp. EC38
8
-
 H2O2 at pH 4.5 and 25°C Hypoxylon sp. EC38
10.4
-
 5-nitro-1,3-benzodioxole at pH 7.0 and 25°C Hypoxylon sp. EC38
11
-
 2,6-dimethoxyphenol at pH 5.0 and 25°C Hypoxylon sp. EC38
17.29
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) at pH 4.5 and 25°C Hypoxylon sp. EC38

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 pH 4.0 is optimal for the conversions of 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) and 3,4-dimethoxybenzyl alcohol Hypoxylon sp. EC38
5
-
 pH 5.0 is optimal for the conversion of 2,6-dimethoxyphenol Hypoxylon sp. EC38
7
-
 the enzyme is optimally active at neutral pH with indole, 5-nitro-1,3-benzodioxole, and benzyl alcohol Hypoxylon sp. EC38

Cofactor

Cofactor Comment Organism Structure
heme
-
 Hypoxylon sp. EC38

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6
-
 benzyl alcohol at pH 7.0 and 25°C Hypoxylon sp. EC38
10
-
 3,4-dimethoxybenzyl alcohol at pH 4.0 and 25°C Hypoxylon sp. EC38
16
-
 indole at pH 7.0 and 25°C Hypoxylon sp. EC38
191
-
 2,6-dimethoxyphenol at pH 5.0 and 25°C Hypoxylon sp. EC38
570
-
 5-nitro-1,3-benzodioxole at pH 7.0 and 25°C Hypoxylon sp. EC38
570
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) at pH 4.5 and 25°C Hypoxylon sp. EC38