Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.11.2.1 extracted from

  • Molina-Espeja, P.; Canellas, M.; Plou, F.J.; Hofrichter, M.; Lucas, F.; Guallar, V.; Alcalde, M.
    Synthesis of 1-naphthol by a natural peroxygenase engineered by directed evolution (2016), ChemBioChem, 17, 341-349 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae Cyclocybe aegerita

Protein Variants

Protein Variants Comment Organism
F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L this mutant retains strong activity and stability, particularly in terms of temperature and the presence of co-solvents, compared to the wild type enzyme Cyclocybe aegerita
G241D/R257K the mutant displays a 2fold improvement in peroxygenase activity and half the peroxidative activity of the wild type enzyme Cyclocybe aegerita

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.048
-
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 4.0, temperature not specified in the publication Cyclocybe aegerita
0.126
-
2,6-dimethoxyphenol mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 7.0, temperature not specified in the publication Cyclocybe aegerita
0.127
-
Naphthalene mutant enzyme G241D/R257K, at pH 7.0, temperature not specified in the publication Cyclocybe aegerita
0.181
-
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme G241D/R257K, at pH 4.0, temperature not specified in the publication Cyclocybe aegerita
0.486
-
H2O2 mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, with 2,6-dimethoxyphenol as cosubstrate, at pH 7.0, temperature not specified in the publication Cyclocybe aegerita
0.578
-
Naphthalene mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 7.0, temperature not specified in the publication Cyclocybe aegerita
0.866
-
2,6-dimethoxyphenol mutant enzyme G241D/R257K, at pH 7.0, temperature not specified in the publication Cyclocybe aegerita
1.25
-
H2O2 mutant enzyme G241D/R257K, with 2,6-dimethoxyphenol as cosubstrate, at pH 7.0, temperature not specified in the publication Cyclocybe aegerita

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Cyclocybe aegerita
-
-

Organism

Organism UniProt Comment Textmining
Cyclocybe aegerita B9W4V6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 2,6-dimethoxyphenol + 2 H2O2
-
Cyclocybe aegerita cerulignone + 2 H2O
-
?
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
Cyclocybe aegerita oxidized 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
?
naphthalene + H2O2
-
Cyclocybe aegerita 1-naphthol + H2O
-
?

Subunits

Subunits Comment Organism
? x * 52000, SDS-PAGE Cyclocybe aegerita

Synonyms

Synonyms Comment Organism
aromatic peroxygenase
-
Cyclocybe aegerita
UPO1
-
Cyclocybe aegerita

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
68
-
2,6-dimethoxyphenol mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 7.0, temperature not specified in the publication Cyclocybe aegerita
78
-
Naphthalene mutant enzyme G241D/R257K, at pH 7.0, temperature not specified in the publication Cyclocybe aegerita
125
-
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme G241D/R257K, at pH 4.0, temperature not specified in the publication Cyclocybe aegerita
142
-
2,6-dimethoxyphenol mutant enzyme G241D/R257K, at pH 7.0, temperature not specified in the publication Cyclocybe aegerita
229
-
Naphthalene mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 7.0, temperature not specified in the publication Cyclocybe aegerita
238
-
H2O2 mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, with 2,6-dimethoxyphenol as cosubstrate, at pH 7.0, temperature not specified in the publication Cyclocybe aegerita
395
-
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 4.0, temperature not specified in the publication Cyclocybe aegerita
447
-
H2O2 mutant enzyme G241D/R257K, with 2,6-dimethoxyphenol as cosubstrate, at pH 7.0, temperature not specified in the publication Cyclocybe aegerita

Cofactor

Cofactor Comment Organism Structure
heme
-
Cyclocybe aegerita

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
160
-
2,6-dimethoxyphenol mutant enzyme G241D/R257K, at pH 7.0, temperature not specified in the publication Cyclocybe aegerita
360
-
H2O2 mutant enzyme G241D/R257K, with 2,6-dimethoxyphenol as cosubstrate, at pH 7.0, temperature not specified in the publication Cyclocybe aegerita
400
-
Naphthalene mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 7.0, temperature not specified in the publication Cyclocybe aegerita
500
-
H2O2 mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, with 2,6-dimethoxyphenol as cosubstrate, at pH 7.0, temperature not specified in the publication Cyclocybe aegerita
540
-
2,6-dimethoxyphenol mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 7.0, temperature not specified in the publication Cyclocybe aegerita
620
-
Naphthalene mutant enzyme G241D/R257K, at pH 7.0, temperature not specified in the publication Cyclocybe aegerita
690
-
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme G241D/R257K, at pH 4.0, temperature not specified in the publication Cyclocybe aegerita
8200
-
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 4.0, temperature not specified in the publication Cyclocybe aegerita