Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae | Cyclocybe aegerita |
Protein Variants | Comment | Organism |
---|---|---|
F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L | this mutant retains strong activity and stability, particularly in terms of temperature and the presence of co-solvents, compared to the wild type enzyme | Cyclocybe aegerita |
G241D/R257K | the mutant displays a 2fold improvement in peroxygenase activity and half the peroxidative activity of the wild type enzyme | Cyclocybe aegerita |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 4.0, temperature not specified in the publication | Cyclocybe aegerita | |
0.126 | - |
2,6-dimethoxyphenol | mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
0.127 | - |
Naphthalene | mutant enzyme G241D/R257K, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
0.181 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme G241D/R257K, at pH 4.0, temperature not specified in the publication | Cyclocybe aegerita | |
0.486 | - |
H2O2 | mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, with 2,6-dimethoxyphenol as cosubstrate, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
0.578 | - |
Naphthalene | mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
0.866 | - |
2,6-dimethoxyphenol | mutant enzyme G241D/R257K, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
1.25 | - |
H2O2 | mutant enzyme G241D/R257K, with 2,6-dimethoxyphenol as cosubstrate, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Cyclocybe aegerita | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyclocybe aegerita | B9W4V6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 2,6-dimethoxyphenol + 2 H2O2 | - |
Cyclocybe aegerita | cerulignone + 2 H2O | - |
? | |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Cyclocybe aegerita | oxidized 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? | |
naphthalene + H2O2 | - |
Cyclocybe aegerita | 1-naphthol + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, SDS-PAGE | Cyclocybe aegerita |
Synonyms | Comment | Organism |
---|---|---|
aromatic peroxygenase | - |
Cyclocybe aegerita |
UPO1 | - |
Cyclocybe aegerita |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
68 | - |
2,6-dimethoxyphenol | mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
78 | - |
Naphthalene | mutant enzyme G241D/R257K, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
125 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme G241D/R257K, at pH 4.0, temperature not specified in the publication | Cyclocybe aegerita | |
142 | - |
2,6-dimethoxyphenol | mutant enzyme G241D/R257K, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
229 | - |
Naphthalene | mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
238 | - |
H2O2 | mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, with 2,6-dimethoxyphenol as cosubstrate, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
395 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 4.0, temperature not specified in the publication | Cyclocybe aegerita | |
447 | - |
H2O2 | mutant enzyme G241D/R257K, with 2,6-dimethoxyphenol as cosubstrate, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Cyclocybe aegerita |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
160 | - |
2,6-dimethoxyphenol | mutant enzyme G241D/R257K, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
360 | - |
H2O2 | mutant enzyme G241D/R257K, with 2,6-dimethoxyphenol as cosubstrate, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
400 | - |
Naphthalene | mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
500 | - |
H2O2 | mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, with 2,6-dimethoxyphenol as cosubstrate, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
540 | - |
2,6-dimethoxyphenol | mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
620 | - |
Naphthalene | mutant enzyme G241D/R257K, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
690 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme G241D/R257K, at pH 4.0, temperature not specified in the publication | Cyclocybe aegerita | |
8200 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 4.0, temperature not specified in the publication | Cyclocybe aegerita |