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Literature summary for 1.11.2.1 extracted from

  • Ullrich, R.; Liers, C.; Schimpke, S.; Hofrichter, M.
    Purification of homogeneous forms of fungal peroxygenase (2009), Biotechnol. J., 4, 1619-1626.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Cyclocybe aegerita
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
SDS-PAGE Cyclocybe aegerita

Organism

Organism UniProt Comment Textmining
Cyclocybe aegerita
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Cyclocybe aegerita

Purification (Commentary)

Purification (Comment) Organism
ultrafiltration, SP Sepharose column chromatography, and Mono P column chromatography Cyclocybe aegerita

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
77
-
major enzyme form P I, after 33fold purification, using veratryl alchol as substrate, pH and temperature not specified in the publication Cyclocybe aegerita
94
-
major enzyme form P II, after 40fold purification, using veratryl alchol as substrate, pH and temperature not specified in the publication Cyclocybe aegerita
103
-
major enzyme form P III, after 44fold purification, using veratryl alchol as substrate, pH and temperature not specified in the publication Cyclocybe aegerita

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
naphthalene + H2O2 naphthalene is regioselectively converted into 1-naphthol and 2-naphthol at a ratio of 12:1 Cyclocybe aegerita 1-naphthol + 2-naphthol + H2O
-
?
toluene + H2O2 all peroxygenase fractions oxygenate toluene at both the side chain and the aromatic ring with a ratio of side chain versus aromatic hydroxylation of about 2:1 in all cases Cyclocybe aegerita benzyl alcohol + benzaldehyde + benzoic acid + 2-cresol + 4-cresol + methylhydroquinone + H2O
-
?
veratryl alcohol + H2O2 + H+
-
Cyclocybe aegerita veratraldehyde + H2O
-
?

Synonyms

Synonyms Comment Organism
aromatic peroxygenase
-
Cyclocybe aegerita

pI Value

Organism Comment pI Value Maximum pI Value
Cyclocybe aegerita the final protein fraction had a molecular mass of 46 kDa but still consists of several incompletely separated proteins with slightly differing isoelectric points (pI 5.2, 5.6, 6.1), probably representing differently glycosylated isoforms
-
5.2
Cyclocybe aegerita the final protein fraction had a molecular mass of 46 kDa but still consists of several incompletely separated proteins with slightly differing isoelectric points (pI 5.2, 5.6, 6.1), probably representing differently glycosylated isoforms
-
5.6
Cyclocybe aegerita the final protein fraction had a molecular mass of 46 kDa but still consists of several incompletely separated proteins with slightly differing isoelectric points (pI 5.2, 5.6, 6.1), probably representing differently glycosylated isoforms
-
6.1