KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | in sodium citrate buffer, pH 4.5, at 25°C | Cyclocybe aegerita | |
0.298 | - |
2,6-dimethoxyphenol | in sodium citrate buffer, pH 7.0, at 25°C | Cyclocybe aegerita | |
0.32 | - |
Naphthalene | in potassium phosphate buffer, pH 7.0, at 25°C | Cyclocybe aegerita | |
1.001 | - |
benzyl alcohol | in sodium citrate buffer, pH 7.0, at 25°C | Cyclocybe aegerita | |
1.313 | - |
H2O2 | in sodium citrate buffer, pH 7.0, at 25°C | Cyclocybe aegerita | |
2.367 | - |
veratryl alcohol | in sodium citrate buffer, pH 7.0, at 25°C | Cyclocybe aegerita |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyclocybe aegerita | - |
- |
- |
Purification (Comment) | Organism |
---|---|
fast protein liquid chromatography | Cyclocybe aegerita |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
74.8 | - |
purified enzyme, using veratryl alcohol as substrate, at pH 7.0 and 25°C | Cyclocybe aegerita |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 2,6-dimethoxyphenol + 2 H2O2 | - |
Cyclocybe aegerita | cerulignone + 2 H2O | - |
? | |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Cyclocybe aegerita | ? | - |
? | |
benzyl alcohol + H2O2 | - |
Cyclocybe aegerita | benzaldehyde + H2O | - |
? | |
naphthalene + H2O2 | - |
Cyclocybe aegerita | 1-naphthol + H2O | in addition to 1-naphthol a smaller amount of naphthalene is converted to 2 naphthol dependent on pH, at pH 7.0-8.0 3% 2-naphthol and 97% 1-naphthol are formed while at pH 3.0 18% 2-naphthol and 82% 1-naphthol are formed. Traces of 1-naphthol are later oxidized to 1,4-naphthoquinone | ? | |
veratryl alcohol + H2O2 + H+ | - |
Cyclocybe aegerita | veratraldehyde + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Agrocybe aegerita peroxidase | P II, main isoform of Agrocybe aegerita peroxidase | Cyclocybe aegerita |
haloperoxidase-peroxygenase | - |
Cyclocybe aegerita |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
85 | - |
veratryl alcohol | in sodium citrate buffer, pH 7.0, at 25°C | Cyclocybe aegerita | |
108 | - |
2,6-dimethoxyphenol | in sodium citrate buffer, pH 7.0, at 25°C | Cyclocybe aegerita | |
166 | - |
Naphthalene | in potassium phosphate buffer, pH 7.0, at 25°C | Cyclocybe aegerita | |
269 | - |
benzyl alcohol | in sodium citrate buffer, pH 7.0, at 25°C | Cyclocybe aegerita | |
283 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | in sodium citrate buffer, pH 4.5, at 25°C | Cyclocybe aegerita | |
367 | - |
H2O2 | in sodium citrate buffer, pH 7.0, at 25°C | Cyclocybe aegerita |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | activity related to 1-naphthol formation shows a broad pH optimum between 5.0 and 8.0 | Cyclocybe aegerita |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | substantial oxygenation of naphthalene still occurs at pH 3.0 and pH 9.0 (60 and 70% of the maximum conversion rate, respectively) | Cyclocybe aegerita |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Cyclocybe aegerita |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
35.8 | - |
veratryl alcohol | in sodium citrate buffer, pH 7.0, at 25°C | Cyclocybe aegerita | |
269 | - |
benzyl alcohol | in sodium citrate buffer, pH 7.0, at 25°C | Cyclocybe aegerita | |
279 | - |
H2O2 | in sodium citrate buffer, pH 7.0, at 25°C | Cyclocybe aegerita | |
361 | - |
2,6-dimethoxyphenol | in sodium citrate buffer, pH 7.0, at 25°C | Cyclocybe aegerita | |
517 | - |
Naphthalene | in potassium phosphate buffer, pH 7.0, at 25°C | Cyclocybe aegerita | |
7670 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | in sodium citrate buffer, pH 4.5, at 25°C | Cyclocybe aegerita |