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Literature summary for 1.11.2.1 extracted from

  • Ullrich, R.; Nuske, J.; Scheibner, K.; Spantzel, J.; Hofrichter, M.
    Novel haloperoxidase from the agaric basidiomycete Agrocybe aegerita oxidizes aryl alcohols and aldehydes (2004), Appl. Environ. Microbiol., 70, 4575-4581.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
H2O2 in the presence of 5 mM veratryl alcohol, optimal activity is observed with 2 mM H2O2, but the enzyme still exhibits 35% of the maximum activity with 10 mM H2O2 Cyclocybe aegerita

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.037
-
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) at pH 4.5 in sodium phosphate/citrate buffer, in the presence of 5 mM benzyl alcohol, at 25°C Cyclocybe aegerita
0.298
-
2,6-dimethoxyphenol at pH 7.0 in sodium phosphate/citrate buffer, at 25°C Cyclocybe aegerita
1.001
-
benzyl alcohol at pH 7.0 in sodium phosphate/citrate buffer, at 25°C Cyclocybe aegerita
1.313
-
H2O2 at pH 7.0 in sodium phosphate/citrate buffer, at 25°C Cyclocybe aegerita
2.367
-
veratryl alcohol at pH 7.0 in sodium phosphate/citrate buffer, at 25°C Cyclocybe aegerita

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
isozymes P I and P II, SDS-PAGE Cyclocybe aegerita

Organism

Organism UniProt Comment Textmining
Cyclocybe aegerita
-
strain TMA1, synonym of Agrocybe cylindracea, black poplar mushroom
-

Purification (Commentary)

Purification (Comment) Organism
Q Sepharose column chromatography, SP Sepharose column chromatography, and Mono S column chromatography Cyclocybe aegerita

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3
-
peroxidase from culture liquid, at pH 7.0 in sodium citrate/phosphate buffer, using veratryl alcohol as substrate, at 25°C Cyclocybe aegerita
71.8
-
purified peroxidase, at pH 2.75 in phosphate buffer, using monochlorodimedone (Cl-) as substrate, at 25°C Cyclocybe aegerita
99.6
-
purified peroxidase, at pH 7.0 in sodium citrate/phosphate buffer, using 2,6-dimethoxyphenol as substrate, at 25°C Cyclocybe aegerita
165
-
peroxidase isozyme P II, after 55fold purification, at pH 7.0 in sodium citrate/phosphate buffer, using veratryl alcohol as substrate, at 25°C Cyclocybe aegerita
167
-
peroxidase isozyme P I, after 56fold purification, at pH 7.0 in sodium citrate/phosphate buffer, using veratryl alcohol as substrate, at 25°C Cyclocybe aegerita
234.2
-
purified peroxidase, at pH 7.0 in sodium citrate/phosphate buffer, using benzyl alcohol as substrate, at 25°C Cyclocybe aegerita
236.7
-
purified peroxidase, at pH 7.0 in sodium citrate/phosphate buffer, using benzaldehyde as substrate, at 25°C Cyclocybe aegerita
295.7
-
purified peroxidase, at pH 5.0 in sodium citrate/phosphate buffer, using 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) as substrate, at 25°C Cyclocybe aegerita
354.3
-
purified peroxidase, at pH 2.75 in phosphate buffer, using monochlorodimedone (Br-) as substrate, at 25°C Cyclocybe aegerita

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) + H2O2
-
Cyclocybe aegerita oxidized 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) + H2O
-
?
2,6-dimethoxyphenol + H2O2
-
Cyclocybe aegerita oxidized 2,6-dimethoxyphenol + H2O
-
?
anisaldehyde + H2O2
-
Cyclocybe aegerita ? + H2O
-
?
anisyl alcohol + H2O2
-
Cyclocybe aegerita anisaldehyde + H2O
-
?
benzaldehyde + H2O2
-
Cyclocybe aegerita benzoic acid + H2O
-
?
benzyl alcohol + H2O2
-
Cyclocybe aegerita benzaldehyde + H2O
-
?
additional information the enzyme exhibits also haloperoxidase activity as shown by the chlorination or bromination of monochlorodimedone. Ethanol is not oxidized by the peroxidase Cyclocybe aegerita ?
-
?
veratraldehyde + H2O2
-
Cyclocybe aegerita veratric acid + H2O
-
?
veratryl alcohol + H2O2 + H+
-
Cyclocybe aegerita veratraldehyde + H2O
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
85
-
veratryl alcohol at pH 7.0 in sodium phosphate/citrate buffer, at 25°C Cyclocybe aegerita
108
-
2,6-dimethoxyphenol at pH 7.0 in sodium phosphate/citrate buffer, at 25°C Cyclocybe aegerita
269
-
benzyl alcohol at pH 7.0 in sodium phosphate/citrate buffer, at 25°C Cyclocybe aegerita
283
-
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) at pH 4.5 in sodium phosphate/citrate buffer, in the presence of 5 mM benzyl alcohol, at 25°C Cyclocybe aegerita
367
-
H2O2 at pH 7.0 in sodium phosphate/citrate buffer, at 25°C Cyclocybe aegerita

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7
-
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) oxidation occurs in a narrow pH range (pH 2.0-6.5), with an acidic maximum around pH 4.7 Cyclocybe aegerita
5.5
-
the enzyme has two pH optima for the oxidation of veratryl alcohol (pH 5.5 and 7.0) Cyclocybe aegerita
6.2
-
the enzyme has two pH optima for the oxidation of benzyl alcohol (pH 6.2 and 7.2) Cyclocybe aegerita
7
-
the enzyme has two pH optima for the oxidation of veratryl alcohol (pH 6.2 and 7.2) Cyclocybe aegerita
7
-
the optimum pH for the oxidation of aryl alcohols is around 7.0 Cyclocybe aegerita
7.2
-
the enzyme has two pH optima for the oxidation of benzyl alcohol (pH 6.2 and 7.2) Cyclocybe aegerita

pH Range

pH Minimum pH Maximum Comment Organism
2.5 9
-
Cyclocybe aegerita

pI Value

Organism Comment pI Value Maximum pI Value
Cyclocybe aegerita isozyme P I, isoelectric focusing 5.4 4.6
Cyclocybe aegerita isozyme P II, isoelectric focusing 5.6 4.9

Expression

Organism Comment Expression
Cyclocybe aegerita highest enzyme levels are detected in the presence of soybean meal (60 g/l) up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
35.8
-
veratryl alcohol at pH 7.0 in sodium phosphate/citrate buffer, at 25°C Cyclocybe aegerita
269
-
benzyl alcohol at pH 7.0 in sodium phosphate/citrate buffer, at 25°C Cyclocybe aegerita
279
-
H2O2 at pH 7.0 in sodium phosphate/citrate buffer, at 25°C Cyclocybe aegerita
361
-
2,6-dimethoxyphenol at pH 7.0 in sodium phosphate/citrate buffer, at 25°C Cyclocybe aegerita
7670
-
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) at pH 4.5 in sodium phosphate/citrate buffer, in the presence of 5 mM benzyl alcohol, at 25°C Cyclocybe aegerita