Activating Compound | Comment | Organism | Structure |
---|---|---|---|
H2O2 | in the presence of 5 mM veratryl alcohol, optimal activity is observed with 2 mM H2O2, but the enzyme still exhibits 35% of the maximum activity with 10 mM H2O2 | Cyclocybe aegerita |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) | at pH 4.5 in sodium phosphate/citrate buffer, in the presence of 5 mM benzyl alcohol, at 25°C | Cyclocybe aegerita | |
0.298 | - |
2,6-dimethoxyphenol | at pH 7.0 in sodium phosphate/citrate buffer, at 25°C | Cyclocybe aegerita | |
1.001 | - |
benzyl alcohol | at pH 7.0 in sodium phosphate/citrate buffer, at 25°C | Cyclocybe aegerita | |
1.313 | - |
H2O2 | at pH 7.0 in sodium phosphate/citrate buffer, at 25°C | Cyclocybe aegerita | |
2.367 | - |
veratryl alcohol | at pH 7.0 in sodium phosphate/citrate buffer, at 25°C | Cyclocybe aegerita |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
isozymes P I and P II, SDS-PAGE | Cyclocybe aegerita |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyclocybe aegerita | - |
strain TMA1, synonym of Agrocybe cylindracea, black poplar mushroom | - |
Purification (Comment) | Organism |
---|---|
Q Sepharose column chromatography, SP Sepharose column chromatography, and Mono S column chromatography | Cyclocybe aegerita |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3 | - |
peroxidase from culture liquid, at pH 7.0 in sodium citrate/phosphate buffer, using veratryl alcohol as substrate, at 25°C | Cyclocybe aegerita |
71.8 | - |
purified peroxidase, at pH 2.75 in phosphate buffer, using monochlorodimedone (Cl-) as substrate, at 25°C | Cyclocybe aegerita |
99.6 | - |
purified peroxidase, at pH 7.0 in sodium citrate/phosphate buffer, using 2,6-dimethoxyphenol as substrate, at 25°C | Cyclocybe aegerita |
165 | - |
peroxidase isozyme P II, after 55fold purification, at pH 7.0 in sodium citrate/phosphate buffer, using veratryl alcohol as substrate, at 25°C | Cyclocybe aegerita |
167 | - |
peroxidase isozyme P I, after 56fold purification, at pH 7.0 in sodium citrate/phosphate buffer, using veratryl alcohol as substrate, at 25°C | Cyclocybe aegerita |
234.2 | - |
purified peroxidase, at pH 7.0 in sodium citrate/phosphate buffer, using benzyl alcohol as substrate, at 25°C | Cyclocybe aegerita |
236.7 | - |
purified peroxidase, at pH 7.0 in sodium citrate/phosphate buffer, using benzaldehyde as substrate, at 25°C | Cyclocybe aegerita |
295.7 | - |
purified peroxidase, at pH 5.0 in sodium citrate/phosphate buffer, using 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) as substrate, at 25°C | Cyclocybe aegerita |
354.3 | - |
purified peroxidase, at pH 2.75 in phosphate buffer, using monochlorodimedone (Br-) as substrate, at 25°C | Cyclocybe aegerita |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) + H2O2 | - |
Cyclocybe aegerita | oxidized 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) + H2O | - |
? | |
2,6-dimethoxyphenol + H2O2 | - |
Cyclocybe aegerita | oxidized 2,6-dimethoxyphenol + H2O | - |
? | |
anisaldehyde + H2O2 | - |
Cyclocybe aegerita | ? + H2O | - |
? | |
anisyl alcohol + H2O2 | - |
Cyclocybe aegerita | anisaldehyde + H2O | - |
? | |
benzaldehyde + H2O2 | - |
Cyclocybe aegerita | benzoic acid + H2O | - |
? | |
benzyl alcohol + H2O2 | - |
Cyclocybe aegerita | benzaldehyde + H2O | - |
? | |
additional information | the enzyme exhibits also haloperoxidase activity as shown by the chlorination or bromination of monochlorodimedone. Ethanol is not oxidized by the peroxidase | Cyclocybe aegerita | ? | - |
? | |
veratraldehyde + H2O2 | - |
Cyclocybe aegerita | veratric acid + H2O | - |
? | |
veratryl alcohol + H2O2 + H+ | - |
Cyclocybe aegerita | veratraldehyde + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
85 | - |
veratryl alcohol | at pH 7.0 in sodium phosphate/citrate buffer, at 25°C | Cyclocybe aegerita | |
108 | - |
2,6-dimethoxyphenol | at pH 7.0 in sodium phosphate/citrate buffer, at 25°C | Cyclocybe aegerita | |
269 | - |
benzyl alcohol | at pH 7.0 in sodium phosphate/citrate buffer, at 25°C | Cyclocybe aegerita | |
283 | - |
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) | at pH 4.5 in sodium phosphate/citrate buffer, in the presence of 5 mM benzyl alcohol, at 25°C | Cyclocybe aegerita | |
367 | - |
H2O2 | at pH 7.0 in sodium phosphate/citrate buffer, at 25°C | Cyclocybe aegerita |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.7 | - |
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) oxidation occurs in a narrow pH range (pH 2.0-6.5), with an acidic maximum around pH 4.7 | Cyclocybe aegerita |
5.5 | - |
the enzyme has two pH optima for the oxidation of veratryl alcohol (pH 5.5 and 7.0) | Cyclocybe aegerita |
6.2 | - |
the enzyme has two pH optima for the oxidation of benzyl alcohol (pH 6.2 and 7.2) | Cyclocybe aegerita |
7 | - |
the enzyme has two pH optima for the oxidation of veratryl alcohol (pH 6.2 and 7.2) | Cyclocybe aegerita |
7 | - |
the optimum pH for the oxidation of aryl alcohols is around 7.0 | Cyclocybe aegerita |
7.2 | - |
the enzyme has two pH optima for the oxidation of benzyl alcohol (pH 6.2 and 7.2) | Cyclocybe aegerita |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 9 | - |
Cyclocybe aegerita |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cyclocybe aegerita | isozyme P I, isoelectric focusing | 5.4 | 4.6 |
Cyclocybe aegerita | isozyme P II, isoelectric focusing | 5.6 | 4.9 |
Organism | Comment | Expression |
---|---|---|
Cyclocybe aegerita | highest enzyme levels are detected in the presence of soybean meal (60 g/l) | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
35.8 | - |
veratryl alcohol | at pH 7.0 in sodium phosphate/citrate buffer, at 25°C | Cyclocybe aegerita | |
269 | - |
benzyl alcohol | at pH 7.0 in sodium phosphate/citrate buffer, at 25°C | Cyclocybe aegerita | |
279 | - |
H2O2 | at pH 7.0 in sodium phosphate/citrate buffer, at 25°C | Cyclocybe aegerita | |
361 | - |
2,6-dimethoxyphenol | at pH 7.0 in sodium phosphate/citrate buffer, at 25°C | Cyclocybe aegerita | |
7670 | - |
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) | at pH 4.5 in sodium phosphate/citrate buffer, in the presence of 5 mM benzyl alcohol, at 25°C | Cyclocybe aegerita |