BRENDA - Enzyme Database show
show all sequences of 1.11.1.B6

Vanadium haloperoxidases from brown algae of the Laminariaceae family

Almeida, M.; Filipe, S.; Humanes, M.; Maia, M.F.; Melo, R.; Severino, N.; da Silva, J.A.; Frausto da Silva, J.J.; Wever, R.; Phytochemistry 57, 633-642 (2001)

Data extracted from this reference:

General Stability
General Stability
Organism
by extensive dialysis with citrate-phosphate buffer in the presence of EDTA the enzyme is inactivated due to removal of the prosthetic group
Laminaria hyperborea
by extensive dialysis with citrate–phosphate buffer in the presence of EDTA the enzyme is inactivated due to removal of the prosthetic group
Laminaria ochroleuca
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
H2O2
pH 6.7, isoenzyme Ls1
Laminaria ochroleuca
0.137
-
H2O2
pH 6.7, isoenzyme Ls2
Laminaria ochroleuca
0.166
-
H2O2
pH 6.7, isoenzyme Lh1
Laminaria hyperborea
0.173
-
H2O2
pH 6.7, isoenzyme Lh2
Laminaria hyperborea
0.217
-
H2O2
pH 6.1, isoenzyme Lh2
Laminaria hyperborea
0.243
-
H2O2
pH 6.1, isoenzyme Ls1
Laminaria ochroleuca
0.273
-
H2O2
pH 6.1, isoenzyme Ls2
Laminaria ochroleuca
0.275
-
H2O2
pH 6.1, isoenzyme Lh1
Laminaria hyperborea
0.285
-
H2O2
pH 5.5, isoenzyme Lh2
Laminaria hyperborea
0.333
-
H2O2
pH 5.5, isoenzyme Ls2
Laminaria ochroleuca
0.334
-
H2O2
pH 5.5, isoenzyme Lh1
Laminaria hyperborea
0.376
-
H2O2
pH 5.5, isoenzyme Ls1
Laminaria ochroleuca
1.3
-
I-
pH 5.5, isoenzyme Ls1
Laminaria ochroleuca
1.9
-
I-
pH 5.5, isoenzyme Lh1
Laminaria hyperborea
1.9
-
I-
pH 5.5, isoenzyme Ls2
Laminaria ochroleuca
2.3
-
I-
pH 5.5, isoenzyme Lh2
Laminaria hyperborea
2.7
-
I-
pH 6.1, isoenzyme Ls2
Laminaria ochroleuca
3.4
-
I-
pH 6.1, isoenzyme Lh1
Laminaria hyperborea
3.4
-
I-
pH 6.1, isoenzyme Ls1
Laminaria ochroleuca
3.5
-
I-
pH 6.7, isoenzyme Lh1
Laminaria hyperborea
3.7
-
I-
pH 6.7, isoenzyme Ls1
Laminaria ochroleuca
3.8
-
I-
pH 6.1, isoenzyme Lh2; pH 6.7, isoenzyme Lh2
Laminaria hyperborea
4.3
-
I-
pH 6.7, isoenzyme Ls2
Laminaria ochroleuca
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Vanadium
the enzyme contains vanadium
Laminaria hyperborea
Vanadium
enzyme contains vanadium
Laminaria ochroleuca
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
x * 65000, SDS-PAGE
Laminaria ochroleuca
67000
-
x * 67000, SDS-PAGE
Laminaria hyperborea
67000
-
x * 67000, SDS-PAGE
Laminaria ochroleuca
Organic Solvent Stability
Organic Solvent
Commentary
Organism
1-propanol
50% v/v, 30 day incubation, about 70% loss of activity of isoenzyme Lh2
Laminaria hyperborea
1-propanol
50% v/v, 30 day incubation, more than 95% loss of activity of isoenzyme Ls1, about 75% loss of activity of isoenzyme Ls2
Laminaria ochroleuca
Acetone
50% v/v, 30 day incubation, about 80% loss of activity of isoenzyme Lh2
Laminaria hyperborea
Acetone
50% v/v, 30 day incubation, more than 90% loss of activity of isoenzyme Ls1 and Ls2
Laminaria ochroleuca
Ethanol
50% v/v, 30 day incubation, about 90% loss of activity of isoenzyme Lh2
Laminaria hyperborea
Ethanol
50% v/v, 30 day incubation, more than 90% loss of activity of isoenzyme Ls1 and Ls2
Laminaria ochroleuca
Methanol
50% v/v, 30 day incubation, about 70% loss of activity of isoenzyme Lh2
Laminaria hyperborea
Methanol
50% v/v, 30 day incubation, more than 90% loss of activity of isoenzyme Ls1 and Ls2
Laminaria ochroleuca
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Laminaria hyperborea
-
-
-
Laminaria ochroleuca
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione
i.e. monochlorodimedone. Isoenzyme Lh2 shows 5% of the activity with iodide + H2O2, isoenzyme Lh1 is inactive
676357
Laminaria hyperborea
?
-
-
-
?
I- + H2O2
-
676357
Laminaria hyperborea
triiodide + ?
-
-
-
?
I- + H2O2
-
676357
Laminaria ochroleuca
triiodide + ?
-
-
-
?
additional information
no monochlorodimedonebromination activity
676357
Laminaria ochroleuca
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 67000, SDS-PAGE
Laminaria hyperborea
?
x * 65000, SDS-PAGE; x * 67000, SDS-PAGE
Laminaria ochroleuca
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
isoenzyme Ls1
Laminaria ochroleuca
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
50
stable
Laminaria hyperborea
30
50
stable
Laminaria ochroleuca
General Stability (protein specific)
General Stability
Organism
by extensive dialysis with citrate-phosphate buffer in the presence of EDTA the enzyme is inactivated due to removal of the prosthetic group
Laminaria hyperborea
by extensive dialysis with citrate–phosphate buffer in the presence of EDTA the enzyme is inactivated due to removal of the prosthetic group
Laminaria ochroleuca
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
H2O2
pH 6.7, isoenzyme Ls1
Laminaria ochroleuca
0.137
-
H2O2
pH 6.7, isoenzyme Ls2
Laminaria ochroleuca
0.166
-
H2O2
pH 6.7, isoenzyme Lh1
Laminaria hyperborea
0.173
-
H2O2
pH 6.7, isoenzyme Lh2
Laminaria hyperborea
0.217
-
H2O2
pH 6.1, isoenzyme Lh2
Laminaria hyperborea
0.243
-
H2O2
pH 6.1, isoenzyme Ls1
Laminaria ochroleuca
0.273
-
H2O2
pH 6.1, isoenzyme Ls2
Laminaria ochroleuca
0.275
-
H2O2
pH 6.1, isoenzyme Lh1
Laminaria hyperborea
0.285
-
H2O2
pH 5.5, isoenzyme Lh2
Laminaria hyperborea
0.333
-
H2O2
pH 5.5, isoenzyme Ls2
Laminaria ochroleuca
0.334
-
H2O2
pH 5.5, isoenzyme Lh1
Laminaria hyperborea
0.376
-
H2O2
pH 5.5, isoenzyme Ls1
Laminaria ochroleuca
1.3
-
I-
pH 5.5, isoenzyme Ls1
Laminaria ochroleuca
1.9
-
I-
pH 5.5, isoenzyme Lh1
Laminaria hyperborea
1.9
-
I-
pH 5.5, isoenzyme Ls2
Laminaria ochroleuca
2.3
-
I-
pH 5.5, isoenzyme Lh2
Laminaria hyperborea
2.7
-
I-
pH 6.1, isoenzyme Ls2
Laminaria ochroleuca
3.4
-
I-
pH 6.1, isoenzyme Lh1
Laminaria hyperborea
3.4
-
I-
pH 6.1, isoenzyme Ls1
Laminaria ochroleuca
3.5
-
I-
pH 6.7, isoenzyme Lh1
Laminaria hyperborea
3.7
-
I-
pH 6.7, isoenzyme Ls1
Laminaria ochroleuca
3.8
-
I-
pH 6.1, isoenzyme Lh2; pH 6.7, isoenzyme Lh2
Laminaria hyperborea
4.3
-
I-
pH 6.7, isoenzyme Ls2
Laminaria ochroleuca
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Vanadium
the enzyme contains vanadium
Laminaria hyperborea
Vanadium
enzyme contains vanadium
Laminaria ochroleuca
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
x * 65000, SDS-PAGE
Laminaria ochroleuca
67000
-
x * 67000, SDS-PAGE
Laminaria hyperborea
67000
-
x * 67000, SDS-PAGE
Laminaria ochroleuca
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
1-propanol
50% v/v, 30 day incubation, about 70% loss of activity of isoenzyme Lh2
Laminaria hyperborea
1-propanol
50% v/v, 30 day incubation, more than 95% loss of activity of isoenzyme Ls1, about 75% loss of activity of isoenzyme Ls2
Laminaria ochroleuca
Acetone
50% v/v, 30 day incubation, about 80% loss of activity of isoenzyme Lh2
Laminaria hyperborea
Acetone
50% v/v, 30 day incubation, more than 90% loss of activity of isoenzyme Ls1 and Ls2
Laminaria ochroleuca
Ethanol
50% v/v, 30 day incubation, about 90% loss of activity of isoenzyme Lh2
Laminaria hyperborea
Ethanol
50% v/v, 30 day incubation, more than 90% loss of activity of isoenzyme Ls1 and Ls2
Laminaria ochroleuca
Methanol
50% v/v, 30 day incubation, about 70% loss of activity of isoenzyme Lh2
Laminaria hyperborea
Methanol
50% v/v, 30 day incubation, more than 90% loss of activity of isoenzyme Ls1 and Ls2
Laminaria ochroleuca
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione
i.e. monochlorodimedone. Isoenzyme Lh2 shows 5% of the activity with iodide + H2O2, isoenzyme Lh1 is inactive
676357
Laminaria hyperborea
?
-
-
-
?
I- + H2O2
-
676357
Laminaria hyperborea
triiodide + ?
-
-
-
?
I- + H2O2
-
676357
Laminaria ochroleuca
triiodide + ?
-
-
-
?
additional information
no monochlorodimedonebromination activity
676357
Laminaria ochroleuca
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 67000, SDS-PAGE
Laminaria hyperborea
?
x * 65000, SDS-PAGE; x * 67000, SDS-PAGE
Laminaria ochroleuca
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
isoenzyme Ls1
Laminaria ochroleuca
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
50
stable
Laminaria hyperborea
30
50
stable
Laminaria ochroleuca
Other publictions for EC 1.11.1.B6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741689
Fournier
The vanadium iodoperoxidase f ...
Zobellia galactanivorans, Zobellia galactanivorans DSM 12802
Appl. Environ. Microbiol.
80
7561-7573
2014
-
-
1
1
12
-
-
13
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
13
-
-
-
-
-
-
-
-
-
1
-
1
12
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
13
-
-
-
-
-
-
-
-
13
13
674915
Colin
Vanadium-dependent iodoperoxid ...
Laminaria digitata
J. Biol. Inorg. Chem.
10
156-166
2005
-
-
-
-
-
-
1
1
-
-
-
-
-
2
-
-
1
-
-
1
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
1
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
676355
Almeida
Purification and characterisat ...
Pelvetia canaliculata
Phytochemistry
54
5-11
2005
-
-
-
-
-
2
1
4
-
1
3
-
-
1
-
1
1
-
-
-
-
-
4
2
-
-
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
2
4
-
1
3
-
-
-
1
1
-
-
-
-
4
2
-
-
4
-
2
-
-
-
-
-
-
-
-
-
676357
Almeida
Vanadium haloperoxidases from ...
Laminaria hyperborea, Laminaria ochroleuca
Phytochemistry
57
633-642
2001
-
-
-
-
-
2
-
23
-
2
3
-
8
2
-
-
-
-
-
-
-
-
4
2
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
23
-
2
3
-
8
-
-
-
-
-
-
-
4
2
1
-
2
-
-
-
-
-
-
-
-
-
-
-