Cloned (Comment) | Organism |
---|---|
3000fold overexpression in Streptomyces aureofaciens Tü24-88 | Streptomyces lividans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | 2.3 | H2O2 | bromination of monochlorodimedone | Streptomyces lividans | |
11 | - |
Br- | bromination of monochlorodimedone | Streptomyces lividans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme contains no metal | Streptomyces lividans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, SDS-PAGE | Streptomyces lividans |
64000 | - |
gel filtration | Streptomyces lividans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces lividans | - |
TK64 | - |
Streptomyces lividans TK64 | - |
TK64 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Streptomyces lividans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Streptomyces lividans |
Storage Stability | Organism |
---|---|
-20°C, sodium acetate buffer, pH 5.5, stable for months | Streptomyces lividans |
6°C, 20 mM sodium acetate buffer, pH 3.5-6, or ammonium acetate buffer, pH 6-8, stable for weeks | Streptomyces lividans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
indole + Br- + H2O2 | - |
Streptomyces lividans | ? | - |
? | |
indole + Br- + H2O2 | - |
Streptomyces lividans TK64 | ? | - |
? | |
indole + Cl- + H2O2 | - |
Streptomyces lividans | oxindole + monochloroindole + H2O | - |
? | |
indole + Cl- + H2O2 | - |
Streptomyces lividans TK64 | oxindole + monochloroindole + H2O | - |
? | |
monochlorodimedon + Cl- + H2O2 | no activity | Streptomyces lividans | dichlorodimedon + H2O | - |
? | |
monochlorodimedon + Cl- + H2O2 | no activity | Streptomyces lividans TK64 | dichlorodimedon + H2O | - |
? | |
monochlorodimedone + Br- + H2O2 | all bacterial nonheme haloperoxidases catalyze the bromination, but not the chlorination of monochlorodimedone. Therefore, they are isolated as bromoperoxidases. While the bromination of organic compounds is very unspecific, a substrate specificity exists for the chlorination. Appropriate substrates such as indole or phenyl pyrrole derivatives are chlorinated | Streptomyces lividans | ? | - |
? | |
monochlorodimedone + Br- + H2O2 | all bacterial nonheme haloperoxidases catalyze the bromination, but not the chlorination of monochlorodimedone. Therefore, they are isolated as bromoperoxidases. While the bromination of organic compounds is very unspecific, a substrate specificity exists for the chlorination. Appropriate substrates such as indole or phenyl pyrrole derivatives are chlorinated | Streptomyces lividans TK64 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, SDS-PAGE | Streptomyces lividans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 5.5 | bromination of monochlorodimedone | Streptomyces lividans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | non-heme chloroperoxidase | Streptomyces lividans |