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Literature summary for 1.11.1.9 extracted from

  • Chafik, A.; Essamadi, A.; Celik, S.; Solak, K.; Mavi, A.
    Characterization of an interesting selenium-dependent glutathione peroxidase (Se-GPx) protecting cells against environmental stress The Camelus dromedarius erythrocytes Se-GPx (2019), Biocatal. Agricult. Biotechnol., 18, 101000 .
No PubMed abstract available
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Activating Compound

Activating Compound Comment Organism Structure
EDTA 2 mM, 128% of initial activity Camelus dromedarius
NaN3 2 mM, 131% of initial activity Camelus dromedarius
PMSF 2 mM, 167% of initial activity Camelus dromedarius
SDS 2 mM, 154% of initial activity Camelus dromedarius

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 2 mM, 23% residual activity Camelus dromedarius
DL-dithiothreitol 2 mM, no residual activity Camelus dromedarius
Fe2+ 2 mM, 21% of initial activity Camelus dromedarius

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 2 mM, 147% of initial activity Camelus dromedarius
Ca2+ 2 mM, 150% of initial activity Camelus dromedarius
Co2+ 2 mM, 184% of initial activity Camelus dromedarius
Mg2+ 2 mM, 154% of initial activity Camelus dromedarius
selenium 3.06 mol of selenium per mol of protein Camelus dromedarius
Zn2+ 2 mM, 166% of initial activity Camelus dromedarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
 gel filtration Camelus dromedarius

Organism

Organism UniProt Comment Textmining
Camelus dromedarius
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by ethanol-chloroform treatment, acetone precipitation and ion exchange chromatography Camelus dromedarius

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Camelus dromedarius
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.09
-
 pH 7, 37°C Camelus dromedarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 glutathione + cumene hydroperoxide 83% of the activity with H2O2 Camelus dromedarius glutathione disulfide + 2-phenylpropan-2-ol + H2O
-
 ?
2 glutathione + H2O2
-
 Camelus dromedarius glutathione disulfide + H2O
-
 ?
2 glutathione + tert-butyl hydroperoxide 87% of the activity with H2O2 Camelus dromedarius glutathione disulfide + tert-butyl alcohol + H2O
-
 ?

Subunits

Subunits Comment Organism
? * 53000 and x * 21000, SDS-PAGE Camelus dromedarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
47
-
-
 Camelus dromedarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Camelus dromedarius