Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.11.1.7 extracted from

  • Abdel-Aty, A.; Salama, W.; El-Badry, M.; Salah, H.; Barakat, A.; Fahmy, A.; Mohamed, S.
    Purification and characterization of peroxidases from garden cress sprouts and their roles in lignification and removal of phenol and p-chlorophenol (2021), J. Food Biochem., 45, e13526 .
    View publication on PubMed
Search for more literature for 1.11.1.7

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 5 mM, 79% inhibition of cationic peroxidase GCP1. 5 mM, 20% inhibition of anionic peroxidase GCP2 Lepidium sativum
Cu2+ 5 mM, 85% inhibition of cationic peroxidase GCP1. 5 mM, 26% inhibition of anionic peroxidase GCP2 Lepidium sativum
Hg2+ 5 mM, 93% inhibition of cationic peroxidase GCP1. 5 mM, 33% inhibition of anionic peroxidase GCP2 Lepidium sativum
Mn2+ 5 mM, 41% inhibition of cationic peroxidase GCP1 Lepidium sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 H2O2 pH 6.0-7.5, 30°C, cationic peroxidase GCP1 Lepidium sativum
1.2
-
 guaiacol pH 6.0-7.5, 30°C, cationic peroxidase GCP1 Lepidium sativum
1.6
-
 H2O2 pH 7.0, 40°C, anionic peroxidase GCP2 Lepidium sativum
3.6
-
 guaiacol pH 7.0, 40°C, anionic peroxidase GCP2 Lepidium sativum

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 5 mM, 1.13fold activation of cationic peroxidase GCP1, 1.3fold activation of anionic peroxidase GCP2 Lepidium sativum
Ca2+ 5 mM, 1.19fold activation of cationic peroxidase GCP1, 1.4fold activation of anionic peroxidase GCP2 Lepidium sativum
Mg2+ 5 mM, 1.42fold activation of anionic peroxidase GCP2 Lepidium sativum
Mn2+ 5 mM, 1.27fold activation of anionic peroxidase GCP2 Lepidium sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 gel filtration, cationic peroxidase GCP1 Lepidium sativum
40000
-
 gel filtration, anionic peroxidase GCP2 Lepidium sativum

Organism

Organism UniProt Comment Textmining
Lepidium sativum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lepidium sativum

Source Tissue

Source Tissue Comment Organism Textmining
sprout
-
 Lepidium sativum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoic acid + H2O2 27% compared to the activity with guaiacol, anionic peroxidase GCP2 Lepidium sativum ? + H2O
-
 ?
benzoic acid + H2O2 activity is 2.13fold higher than activity with guaiacol, cationic peroxidase GCP1 Lepidium sativum ? + H2O
-
 ?
catechin + H2O2 1.7% compared to the activity with guaiacol, cationic peroxidase GCP1 Lepidium sativum ? + H2O
-
 ?
catechin + H2O2 3.5% compared to the activity with guaiacol, anionic peroxidase GCP2 Lepidium sativum ? + H2O
-
 ?
catechol + H2O2 4.5% compared to the activity with guaiacol, cationic peroxidase GCP1 Lepidium sativum ? + H2O
-
 ?
catechol + H2O2 5.1% compared to the activity with guaiacol, anionic peroxidase GCP2 Lepidium sativum ? + H2O
-
 ?
cinnamic acid + H2O2 15% compared to the activity with guaiacol, cationic peroxidase GCP1 Lepidium sativum ? + H2O
-
 ?
cinnamic acid + H2O2 activity is 1.18fold higher than activity with guaiacol, anionic peroxidase GCP2 Lepidium sativum ? + H2O
-
 ?
coniferyl alcohol + H2O2 18% compared to the activity with guaiacol, cationic peroxidase GCP1 Lepidium sativum ? + H2O
-
 ?
coniferyl alcohol + H2O2 activity is 1.27fold higher than activity with guaiacol, anionic peroxidase GCP2 Lepidium sativum ? + H2O
-
 ?
ferulic acid + H2O2 17% compared to the activity with guaiacol, cationic peroxidase GCP1 Lepidium sativum ? + H2O
-
 ?
ferulic acid + H2O2 83.3% compared to the activity with guaiacol, anionic peroxidase GCP2 Lepidium sativum ? + H2O
-
 ?
guaiacol + H2O2
-
 Lepidium sativum tetraguaiacol + H2O
-
 ?
m-phenylenediamine + H2O2 12% compared to the activity with guaiacol, cationic peroxidase GCP1 Lepidium sativum ? + H2O
-
 ?
m-phenylenediamine + H2O2 13.5% compared to the activity with guaiacol, anionic peroxidase GCP2 Lepidium sativum ? + H2O
-
 ?
o-dianisidine + H2O2 25% compared to the activity with guaiacol, anionic peroxidase GCP2 Lepidium sativum ? + H2O
-
 ?
o-dianisidine + H2O2 45% compared to the activity with guaiacol, cationic peroxidase GCP1 Lepidium sativum ? + H2O
-
 ?
o-phenylenediamine + H2O2 activity is 2.37fold higher than activity with guaiacol, anionic peroxidase GCP2 Lepidium sativum ? + H2O
-
 ?
o-phenylenediamine + H2O2 activity is 3.14fold higher than activity with guaiacol, cationic peroxidase GCP1 Lepidium sativum ? + H2O
-
 ?
p-aminoantipyrine + H2O2 26.5% compared to the activity with guaiacol, cationic peroxidase GCP1 Lepidium sativum ? + H2O
-
 ?
p-aminoantipyrine + H2O2 31% compared to the activity with guaiacol, anionic peroxidase GCP2 Lepidium sativum ? + H2O
-
 ?
p-hydroxybenzoic acid + H2O2 5.0% compared to the activity with guaiacol, anionic peroxidase GCP2 Lepidium sativum ? + H2O
-
 ?
p-hydroxybenzoic acid + H2O2 activity is 1.41fold higher than activity with guaiacol, cationic peroxidase GCP1 Lepidium sativum ? + H2O
-
 ?
pyrogallol + H2O2 7.4% compared to the activity with guaiacol, anionic peroxidase GCP2 Lepidium sativum ? + H2O
-
 ?
pyrogallol + H2O2 88% compared to the activity with guaiacol, cationic peroxidase GCP1 Lepidium sativum ? + H2O
-
 ?
sinapyl alcohol + H2O2 30% compared to the activity with guaiacol, cationic peroxidase GCP1 Lepidium sativum ? + H2O
-
 ?
sinapyl alcohol + H2O2 activity is 1.32fold higher than activity with guaiacol, anionic peroxidase GCP2 Lepidium sativum ? + H2O
-
 ?
tannic acid + H2O2 3.8% compared to the activity with guaiacol, cationic peroxidase GCP1 Lepidium sativum ? + H2O
-
 ?
tannic acid + H2O2 78% compared to the activity with guaiacol, anionic peroxidase GCP2 Lepidium sativum ? + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 25000, SDS-PAGE, cationic peroxidase GCP1 Lepidium sativum
monomer 1 * 40000, gel filtration, anionic peroxidase GCP2 Lepidium sativum

Synonyms

Synonyms Comment Organism
GCP1
-
 Lepidium sativum
GCP2
-
 Lepidium sativum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 cationic peroxidase GCP1 Lepidium sativum
40
-
 anionic peroxidase GCP2 Lepidium sativum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 20°C: about 95% of maximal activity, 60°C: about 50% of maximal activity, cationic peroxidase GCP1 Lepidium sativum
20 70 20°C: about 95% of maximal activity, 70°C: about 55% of maximal activity, anionic peroxidase GCP2 Lepidium sativum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, cationic peroxidase GCP1 and anionic peroxidase GCP2 retain 100% of their activities Lepidium sativum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7.5 cationic peroxidase GCP1 Lepidium sativum
7
-
 anionic peroxidase GCP2 Lepidium sativum

pH Range

pH Minimum pH Maximum Comment Organism
4 8 pH 4.0: about 35% of maximal activity, pH 8.0: about 60% of maximal activity, anionic peroxidase GCP2 Lepidium sativum
4.5 8.5 pH 4.5: about 40% of maximal activity, pH 8.5: about 45% of maximal activity, cationic peroxidase GCP1 Lepidium sativum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8 2 h, no loss of activity, cationic peroxidase CGP1 Lepidium sativum
6 7.5 2 h, no loss of activity, anionic peroxidase CGP2 Lepidium sativum

General Information

General Information Comment Organism
physiological function important antioxidant enzyme, which is elevated when plants are subjected to pollution. GCP2 may play an important role in lignification process and removal of phenol and p-chlorophenol from polluted soil/wastewater. It resists the harmful effect of heavy metals. Cationic peroxidase GCP1, as a natural scavenger, has high affinity toward H2O2 coupled to oxidation of some plant phenolic compounds suggesting its role in consuming of excess H2O2 Lepidium sativum