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Literature summary for 1.11.1.7 extracted from
- Peroxidase from jackfruit purification, characterization and thermal inactivation (2018), Int. J. Biol. Macromol., 114, 898-905 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| benzoic acid | 1 mM, 1.1fold activation | Artocarpus heterophyllus |  |
| Citric acid | 1 mM, 1.05fold activation | Artocarpus heterophyllus | |
| EDTA | 1 mM, 1.05fold activation | Artocarpus heterophyllus | |
| malic acid | 1 mM, 1.07fold activation | Artocarpus heterophyllus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ascorbic acid | 1 mM, 82% inhibition | Artocarpus heterophyllus | |
| beta-cyclodextrin | 1 mM, 16% inhibition | Artocarpus heterophyllus | |
| beta-mercaptoethanol | 4 mM, 80% without substrate, 78% inhibition in presence of substrate | Artocarpus heterophyllus | |
| Ca2+ | 5 mM, 17% inhibition | Artocarpus heterophyllus | |
| Cu2+ | 61% inhibition | Artocarpus heterophyllus | |
| cysteine | 1 mM, 28% inhibition | Artocarpus heterophyllus | |
| diethyl dicarbonate | 4 mM, 50% without substrate, 21% inhibition in presence of substrate | Artocarpus heterophyllus | |
| glutathione | 1 mM, 79% inhibition | Artocarpus heterophyllus | |
| K+ | 5 mM, 8% inhibition | Artocarpus heterophyllus | |
| Mg2+ | 5 mM, 7% inhibition | Artocarpus heterophyllus | |
| Mn2+ | 7% inhibition | Artocarpus heterophyllus | |
| PCMB | 4 mM, 78% without substrate, 75% inhibition in presence of substrate | Artocarpus heterophyllus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
H2O2 | pH 6.0, temperature not specified in the publication, cosubstrate: gallic acid | Artocarpus heterophyllus | |
| 0.2 | - |
H2O2 | pH 6.0, temperature not specified in the publication, cosubstrate: guaiacol | Artocarpus heterophyllus | |
| 0.5 | - |
H2O2 | pH 6.0, temperature not specified in the publication, cosubstrate: o-phenylenediamine | Artocarpus heterophyllus | |
| 1.3 | - |
gallic acid | pH 6.0, temperature not specified in the publication | Artocarpus heterophyllus | |
| 3.7 | - |
guaiacol | pH 6.0, temperature not specified in the publication | Artocarpus heterophyllus | |
| 5.9 | - |
o-phenylenediamine | pH 6.0, temperature not specified in the publication | Artocarpus heterophyllus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 5 mM, 1.23fold activation | Artocarpus heterophyllus | |
| Zn2+ | 5 mM, 1.45fold activation | Artocarpus heterophyllus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 104000 | - |
gel filtration | Artocarpus heterophyllus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Artocarpus heterophyllus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, hydrophobic chromatography Octyl-Sepharose 4 Fast Flow column, and molecular exclusion chromatography Sephacryl S-200 HR column | Artocarpus heterophyllus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bulb | - |
Artocarpus heterophyllus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| gallic acid + H2O2 | - |
Artocarpus heterophyllus | ? + H2O | - |
? | |
| guaiacol + H2O2 | - |
Artocarpus heterophyllus | tetraguaiacol + H2O | - |
? | |
| o-phenylenediamine + H2O2 | most suitable substrate | Artocarpus heterophyllus | ? + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 48000, SDS-PAGE | Artocarpus heterophyllus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| POD | - |
Artocarpus heterophyllus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
substrate: o-phenylenediamine | Artocarpus heterophyllus |
| 60 | - |
substrate: guaiacol or gallic acid | Artocarpus heterophyllus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
t1/2: 71.7 min | Artocarpus heterophyllus |
| 75 | - |
t1/2: 25.1 min | Artocarpus heterophyllus |
| 80 | - |
t1/2: 21.8 min | Artocarpus heterophyllus |
| 85 | - |
t1/2: 5.6 min | Artocarpus heterophyllus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Artocarpus heterophyllus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Artocarpus heterophyllus | isoelectric focusing | - |
7.5 |
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