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Literature summary for 1.11.1.7 extracted from
- Combining protein and strain engineering for the production of glyco-engineered horseradish peroxidase C1A in Pichia pastoris (2015), Int. J. Mol. Sci., 16, 23127-23142 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | protein engineering to obtain an active and stable HRP variant with reduced surface glycosylation during production in Pichia pastoris. Mutant N13D/N57S/N255D/N268D produced in the Pichia pastoris benchmark strain shows considerable catalytic activity and thermal stability and is less glycosylated | Armoracia rusticana |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Armoracia rusticana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N13D/N57S/N255D/N268D | mutations introduced to reduce glycosylation during production in Pichia pastoris | Armoracia rusticana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.009 | - |
H2O2 | wild-type, pH 6.5, 30°C | Armoracia rusticana |  |
| 0.015 | - |
H2O2 | mutant N13D/N57S/N255D/N268D, pH 6.5, 30°C | Armoracia rusticana | |
| 0.99 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant N13D/N57S/N255D/N268D, pH 6.5, 30°C | Armoracia rusticana | |
| 1.5 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | wild-type, pH 6.5, 30°C | Armoracia rusticana | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Armoracia rusticana | P00433 | isoform C1A | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Armoracia rusticana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Armoracia rusticana | ? + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65000, SDS-PAGE, wild-type. x * 50000, SDS-PAGE, glycosylation mutant N13D/N57S/N255D/N268D | Armoracia rusticana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HRP C1A | - |
Armoracia rusticana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
half-life of wild-type 31.5 min, half-life of mutant N13D/N57S/N255D/N268D 173 min | Armoracia rusticana |
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Release 2023.1 (January 2023)
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