Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C317A | the heme content of the mutant is very low and the mutant loses the ability to hydroxylate 3-methyltyrosine | Escherichia coli |
H191A | the mutant loses the ability to hydroxylate 3-methyltyrosine | Escherichia coli |
H274A | the mutant loses the ability to hydroxylate 3-methyltyrosine | Escherichia coli |
H313A | the heme content of the mutant is very low and the mutant loses the ability to hydroxylate 3-methyltyrosine | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.64 | - |
3-methyltyrosine | in 100 mM Tris-HCl, pH 9.0, at 25°C | Escherichia coli | |
1 | - |
L-tyrosine | in 100 mM Tris-HCl, pH 9.0, at 25°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39800 | - |
x * 39800, SDS-PAGE | Escherichia coli |
39855 | - |
x * 39855, LC-MS analysis and calculated from amino acid sequence | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methyltyrosine + H2O2 | Escherichia coli | - |
3-hydroxy-5-methyltyrosine + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methyltyrosine + H2O2 | - |
Escherichia coli | 3-hydroxy-5-methyltyrosine + H2O | - |
? | |
L-tyrosine + H2O2 | - |
Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39800, SDS-PAGE | Escherichia coli |
? | x * 39855, LC-MS analysis and calculated from amino acid sequence | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
SacD | - |
Escherichia coli |
SfmD | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.3 | - |
L-tyrosine | in 100 mM Tris-HCl, pH 9.0, at 25°C | Escherichia coli | |
17.8 | - |
3-methyltyrosine | in 100 mM Tris-HCl, pH 9.0, at 25°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the biosynthesis of saframycin A | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.2 | - |
L-tyrosine | in 100 mM Tris-HCl, pH 9.0, at 25°C | Escherichia coli | |
27.8 | - |
3-methyltyrosine | in 100 mM Tris-HCl, pH 9.0, at 25°C | Escherichia coli |