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Literature summary for 1.11.1.7 extracted from

  • Tang, M.C.; Fu, C.Y.; Tang, G.L.
    Characterization of SfmD as a heme peroxidase that catalyzes the regioselective hydroxylation of 3-methyltyrosine to 3-hydroxy-5-methyltyrosine in saframycin A biosynthesis (2012), J. Biol. Chem., 287, 5112-5121.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
C317A the heme content of the mutant is very low and the mutant loses the ability to hydroxylate 3-methyltyrosine Escherichia coli
H191A the mutant loses the ability to hydroxylate 3-methyltyrosine Escherichia coli
H274A the mutant loses the ability to hydroxylate 3-methyltyrosine Escherichia coli
H313A the heme content of the mutant is very low and the mutant loses the ability to hydroxylate 3-methyltyrosine Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.64
-
3-methyltyrosine in 100 mM Tris-HCl, pH 9.0, at 25°C Escherichia coli
1
-
L-tyrosine in 100 mM Tris-HCl, pH 9.0, at 25°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39800
-
x * 39800, SDS-PAGE Escherichia coli
39855
-
x * 39855, LC-MS analysis and calculated from amino acid sequence Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-methyltyrosine + H2O2 Escherichia coli
-
3-hydroxy-5-methyltyrosine + H2O
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methyltyrosine + H2O2
-
Escherichia coli 3-hydroxy-5-methyltyrosine + H2O
-
?
L-tyrosine + H2O2
-
Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
? x * 39800, SDS-PAGE Escherichia coli
? x * 39855, LC-MS analysis and calculated from amino acid sequence Escherichia coli

Synonyms

Synonyms Comment Organism
SacD
-
Escherichia coli
SfmD
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.3
-
L-tyrosine in 100 mM Tris-HCl, pH 9.0, at 25°C Escherichia coli
17.8
-
3-methyltyrosine in 100 mM Tris-HCl, pH 9.0, at 25°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
heme
-
Escherichia coli

General Information

General Information Comment Organism
metabolism the enzyme is involved in the biosynthesis of saframycin A Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
12.2
-
L-tyrosine in 100 mM Tris-HCl, pH 9.0, at 25°C Escherichia coli
27.8
-
3-methyltyrosine in 100 mM Tris-HCl, pH 9.0, at 25°C Escherichia coli