General Stability | Organism |
---|---|
the enzyme has the highest activity in 0.1 M phosphate buffer | Raphanus sativus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0084 | - |
H2O2 | pH 6.0, 30°C | Raphanus sativus | |
0.036 | - |
guaiacol | pH 6.0, 30°C | Raphanus sativus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
calculated from SDS-PAGE | Raphanus sativus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Raphanus sativus | - |
Turkish black radish | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, dialysis, and CM-Sephadex A-50 gel filtration | Raphanus sativus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
using as guaiacol substrate, the specific activity of the crude and 9.7fold enzyme is 24158.4 units/mg and 235051.6 units/mg, respectively. One unit of enzyme activity is defined as 0.01 change of absorbance at 470 nm | Raphanus sativus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guaiacol + H2O2 | - |
Raphanus sativus | tetraguaiacol + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
donor: hydrogen peroxide oxidoreductase | - |
Raphanus sativus |
POD | - |
Raphanus sativus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Raphanus sativus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 80 | the enzyme remains stable at 60°C for 60 min, POD has no activity above 80°C | Raphanus sativus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Raphanus sativus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | POD is more stable at pH ranged 5.0-9.0 in 0.1 M phosphate and 0.1 M Tris/HCl buffer at the end of 8 days | Raphanus sativus |