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Literature summary for 1.11.1.7 extracted from

  • Sisecioglu, M.; Guelcin, I.; Cankaya, M.; Atasever, A.; Sehitoglu, M.; Kaya, H.; Oezdemir, H.
    Purification and characterization of peroxidase from Turkish black radish (Raphanus sativus L.) (2010), J. Med. Plant Res., 4, 1187-1196.
No PubMed abstract available

General Stability

General Stability Organism
the enzyme has the highest activity in 0.1 M phosphate buffer Raphanus sativus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0084
-
H2O2 pH 6.0, 30°C Raphanus sativus
0.036
-
guaiacol pH 6.0, 30°C Raphanus sativus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
calculated from SDS-PAGE Raphanus sativus

Organism

Organism UniProt Comment Textmining
Raphanus sativus
-
Turkish black radish
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, dialysis, and CM-Sephadex A-50 gel filtration Raphanus sativus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
using as guaiacol substrate, the specific activity of the crude and 9.7fold enzyme is 24158.4 units/mg and 235051.6 units/mg, respectively. One unit of enzyme activity is defined as 0.01 change of absorbance at 470 nm Raphanus sativus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guaiacol + H2O2
-
Raphanus sativus tetraguaiacol + H2O
-
?

Synonyms

Synonyms Comment Organism
donor: hydrogen peroxide oxidoreductase
-
Raphanus sativus
POD
-
Raphanus sativus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Raphanus sativus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 80 the enzyme remains stable at 60°C for 60 min, POD has no activity above 80°C Raphanus sativus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Raphanus sativus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 POD is more stable at pH ranged 5.0-9.0 in 0.1 M phosphate and 0.1 M Tris/HCl buffer at the end of 8 days Raphanus sativus