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Literature summary for 1.11.1.7 extracted from

  • Lavery, C.B.; Macinnis, M.C.; Macdonald, M.J.; Williams, J.B.; Spencer, C.A.; Burke, A.A.; Irwin, D.J.; D'Cunha, G.B.
    Purification of peroxidase from horseradish (Armoracia rusticana) roots (2010), J. Agric. Food Chem., 58, 8471-8476.
    View publication on PubMed
Search for more literature for 1.11.1.7

General Stability

General Stability Organism
the optimal buffer for the assay of purified HRP is 50 mM phosphate buffer (pH 7.5) Armoracia rusticana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 SDS-PAGE Armoracia rusticana

Organism

Organism UniProt Comment Textmining
Armoracia rusticana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ultrasonication, ammonium sulfate salt precipitation, and phenyl Sepharose column chromatography Armoracia rusticana

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Armoracia rusticana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.65
-
 crude enzyme, using guaiacol as substrate, in 50 mM phosphate buffer (pH 7.5), at 30°C Armoracia rusticana
772.3
-
 after 291fold purification, using guaiacol as substrate, in 50 mM phosphate buffer (pH 7.5), at 30°C Armoracia rusticana

Storage Stability

Storage Stability Organism
-20°C, 50 mM phosphate buffer (pH 7.5) containing 20% (v/v) glycerol, at least 6 months, less than 5% loss of activity Armoracia rusticana
0-2°C, 50 mM phosphate buffer (pH 7.5), 48-72 h, 40-45% loss of activity Armoracia rusticana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chlorophenol + H2O2 optimal concentrations of 2-chlorophenol and H2O2 are 0.2 mM and 0.3 mM, respectively Armoracia rusticana ?
-
 r
guaiacol + H2O2 optimal concentrations of guaiacol and H2O2 are 0.5 mM and 0.3 mM, respectively Armoracia rusticana tetraguaiacol + H2O
-
 r
phenol + H2O2 optimal concentrations of phenol and H2O2 are 0.2 mM and 0.3 mM, respectively Armoracia rusticana ?
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Armoracia rusticana

Synonyms

Synonyms Comment Organism
HRP
-
 Armoracia rusticana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 optimum temperature using phenol or 2-chlorophenol as substrate Armoracia rusticana
30
-
 optimum temperature using guaiacol as substrate Armoracia rusticana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
42
-
 thermal denaturation of isolated HRP occurs at temperatures above 42°C, and the enzyme activity decreases rapidly Armoracia rusticana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Armoracia rusticana

pH Range

pH Minimum pH Maximum Comment Organism
6 8 although peroxidase activity is comparable in the pH range of 6.0-7.5, significant decline in enzyme activity is observed when the pH is increased to above 8.0 Armoracia rusticana

Cofactor

Cofactor Comment Organism Structure
heme
-
 Armoracia rusticana

pI Value

Organism Comment pI Value Maximum pI Value
Armoracia rusticana isoelectric focusing
-
 8.8