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Literature summary for 1.11.1.7 extracted from

  • Gabrielsen, M.; Zetterstroem, C.E.; Wang, D.; Beckham, K.S.; Elofsson, M.; Isaacs, N.W.; Roe, A.J.
    Expression, purification, crystallization and initial X-ray diffraction analysis of thiol peroxidase from Yersinia pseudotuberculosis (2010), Acta Crystallogr. Sect. F, 66, 1606-1609.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (deltaDE3) cells Yersinia pseudotuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 20% (w/v) PEG 3350, 0.2 M sodium citrate tribasic or 0.1 M sodium malonate pH 5, 12% (w/v) PEG 3350, or 12% (w/v) PEG 3350, 0.05 M Na-HEPES pH 7.0, 1% (w/v) tryptone Yersinia pseudotuberculosis

Protein Variants

Protein Variants Comment Organism
C61S catalytically inactive Yersinia pseudotuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17590
-
 calculated from amino acid sequence Yersinia pseudotuberculosis

Organism

Organism UniProt Comment Textmining
Yersinia pseudotuberculosis Q66A71
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ HisTrap column chromatography Yersinia pseudotuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guaiacol + H2O2
-
 Yersinia pseudotuberculosis tetraguaiacol + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer x-ray crystallography Yersinia pseudotuberculosis

Synonyms

Synonyms Comment Organism
thiol peroxidase
-
 Yersinia pseudotuberculosis
Tpx Tpx is an atypical 2-Cys peroxiredoxin Yersinia pseudotuberculosis

Cofactor

Cofactor Comment Organism Structure
heme
-
 Yersinia pseudotuberculosis