Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C61S | no activity | Escherichia coli |
C82S | 72% of wild-type activity | Escherichia coli |
C82S/C95S | 10% of wild-type activity | Escherichia coli |
C95S | 20% of wild-type activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
cumene hydroperoxide | 25°C, pH 7.0 | Escherichia coli | |
0.023 | - |
thioredoxin 1 | 25°C, pH 7.0 | Escherichia coli | |
1.7 | - |
H2O2 | 25°C, pH 7.0 | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17700 | - |
2 * 17700, ESI-mass spectrometry, dimer in solution | Escherichia coli |
29500 | - |
sedimentation equilibrium, oxidized enzyme | Escherichia coli |
32000 | - |
sedimentation equilibrium, reduced enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A862 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Phenyl-Sepharodse, Q-Sepharose | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced thioredoxin 1 + cumene hydroperoxide | - |
Escherichia coli | oxidized thioredoxin 1 + cumene hydroxide | - |
? | |
reduced thioredoxin 1 + H2O2 | - |
Escherichia coli | oxidized thioredoxin 1 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 17700, ESI-mass spectrometry, dimer in solution | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
p20 | - |
Escherichia coli |
scavengase | - |
Escherichia coli |
thiol peroxidase | - |
Escherichia coli |
Tpx | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
70.1 | - |
cumene hydroperoxide | 25°C, pH 7.0 | Escherichia coli | |
76 | - |
H2O2 | 25°C, pH 7.0 | Escherichia coli |