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Literature summary for 1.11.1.6 extracted from

  • Tan, Q.; Song, Q.; Zhang, Y.; Wei, D.
    Characterization and application of D-amino acid oxidase and catalase within permeabilized Pichia pastoris cells in bioconversions (2007), Appl. Biochem. Biotechnol., 136, 279-289.
    View publication on PubMed

Application

Application Comment Organism
synthesis high-density fermentation of recombinant Pichia pastoris in a 1-l-fermentor yields 684800 U/l of catalase, measured with permeabilized cells. Catalase shows a half-life of 4 days at 30°C and cells can be reused for synthesis up to 13 times. Permeabilized cells co-expressing catalase and D-amino acid oxidase convert D-phenylalanine into 99% phenylpyruvate within 100 min. In a batchwise conversion of cephalosporin C, about 90% 7-beta-(4-carboxybutanamido)-cephalosporanic acid is obtained at each cycle Komagataella pastoris

Organism

Organism UniProt Comment Textmining
Komagataella pastoris
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recombinant enzyme
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