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Literature summary for 1.11.1.5 extracted from

  • Frato, K.E.; Walsh, K.A.; Elliott, S.J.
    Functionally distinct bacterial cytochrome c peroxidases proceed through a common (electro)catalytic intermediate (2016), Biochemistry, 55, 125-132 .
    View publication on PubMed
Search for more literature for 1.11.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Nitrosomonas europaea
expression in Escherichia coli Shewanella oneidensis

Protein Variants

Protein Variants Comment Organism
E123D distal heme pocket mutant, no detectable electrocatalytic turnover of substrate in the highpotential regime Shewanella oneidensis
E123Q distal heme pocket mutant, no detectable electrocatalytic turnover of substrate in the highpotential regime Shewanella oneidensis
F102W distal heme pocket mutant, 10fold decrease in peroxidase activity and high-potential catalytic turnover of hydrogen peroxide Shewanella oneidensis
F81W modest changes in in vitro peroxidase assays Nitrosomonas europaea
H81G mutant is highly active Shewanella oneidensis
Q113N distal heme pocket mutant, no detectable electrocatalytic turnover of substrate in the highpotential regime Shewanella oneidensis

Inhibitors

Inhibitors Comment Organism Structure
L-ascorbate poising the enzyme in the active state using sodium L-ascorbate, allows for capturing a highly active state that turns over peroxide in a high potential regime Nitrosomonas europaea
L-ascorbate poising the enzyme in the active state using sodium L-ascorbate, allows for capturing a highly active state that turns over peroxide in a high potential regime Shewanella oneidensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00013
-
 H2O2 mutant F102W, pH 6.0, 23°C Nitrosomonas europaea
0.0004
-
 H2O2 mutant F81W, pH 6.0, 23°C Nitrosomonas europaea
0.004
-
 H2O2 mutant F102W, pH 6.0, 23°C Shewanella oneidensis

Organism

Organism UniProt Comment Textmining
Nitrosomonas europaea
-
-
-
Shewanella oneidensis Q8EF24
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferrocytochrome c + H2O2
-
 Nitrosomonas europaea 2 ferricytochrome c + 2 H2O
-
 ?
2 ferrocytochrome c + H2O2
-
 Shewanella oneidensis 2 ferricytochrome c + 2 H2O
-
 ?
additional information the rate-limiting step involves a proton-coupled single electron reduction of a high valent iron species centered on the low-potential heme. Reduction shifts the pKa's of at least two amino acids. Loop 1 shifts during the rate-limiting step, changing the environment of residue His81 Shewanella oneidensis ?
-
 ?

Synonyms

Synonyms Comment Organism
diheme cytochrome c5 peroxidase CcpA
-
 Nitrosomonas europaea
diheme cytochrome c5 peroxidase CcpA
-
 Shewanella oneidensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7
-
 H2O2 mutant F81W, pH 6.0, 23°C Nitrosomonas europaea
4.3
-
 H2O2 wild-type, pH 6.0, 23°C Nitrosomonas europaea
5.5
-
 H2O2 mutant F102W, pH 6.0, 23°C Shewanella oneidensis