Literature summary for 1.11.1.5 extracted from

Seidel, J.; Hoffmann, M.; Ellis, K.E.; Seidel, A.; Spatzal, T.; Gerhardt, S.; Elliott, S.J.; Einsle, O.
MacA is a second cytochrome c peroxidase of Geobacter sulfurreducens (2012), Biochemistry, 51, 2747-2756.

Cloned(Commentary)

Cloned (Comment)	Organism
gene macA, expression as N-terminally Strep-tagged protein in Escherichia coli strain BL21(DE3)	Geobacter sulfurreducens

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant Strep-tagged mutant enzyme H93G, and wild-type enzyme in reduced, oxidized, and semireduced states, sitting drop vapor diffusion method, 0.001 ml of 7.5 mg/ml protein is mixed with 0.001 ml of reservoir solution containing for the oxidized enzyme 0.1 M ammonium acetate buffer, pH 5.5, 1.3 M Na/K phosphate and 6% v/v of ethanol, for the ascorbate reduced enzyme 0.1 M HEPES/NaOH buffer, pH 7.5, 0.2 M ammonium acetate, and 25% w/v PEG 3350, and for the dithionite reduced enzyme 0.1 M sodium citrate buffer, pH 5.6, and 1 M ammonium phosphate, mutant MacA_H93G crystals are obtained in 1 M ammonium sulfate, pH 5.4, X-ray diffraction structure determination and analysis at 1.2-2.3 A resolution	Geobacter sulfurreducens

Crystallization (Comment)

Organism

purified recombinant Strep-tagged mutant enzyme H93G, and wild-type enzyme in reduced, oxidized, and semireduced states, sitting drop vapor diffusion method, 0.001 ml of 7.5 mg/ml protein is mixed with 0.001 ml of reservoir solution containing for the oxidized enzyme 0.1 M ammonium acetate buffer, pH 5.5, 1.3 M Na/K phosphate and 6% v/v of ethanol, for the ascorbate reduced enzyme 0.1 M HEPES/NaOH buffer, pH 7.5, 0.2 M ammonium acetate, and 25% w/v PEG 3350, and for the dithionite reduced enzyme 0.1 M sodium citrate buffer, pH 5.6, and 1 M ammonium phosphate, mutant MacA_H93G crystals are obtained in 1 M ammonium sulfate, pH 5.4, X-ray diffraction structure determination and analysis at 1.2-2.3 A resolution

Geobacter sulfurreducens

Protein Variants

Protein Variants	Comment	Organism
H93G	site-directed mutagenesis	Geobacter sulfurreducens
M297H	site-directed mutagenesis	Geobacter sulfurreducens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	heme iron Fe(III)	Geobacter sulfurreducens

Organism

Organism	UniProt	Comment	Textmining
Geobacter sulfurreducens	Q74FY6	gene macA, i.e. gsu0466	-
Geobacter sulfurreducens DSM 12127	Q74FY6	gene macA, i.e. gsu0466	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally Strep-tagged protein from Escherichia coli strain BL21(DE3) by streptactin affinity chromatography and gel filtration	Geobacter sulfurreducens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,2'-azino-bis(3-ethylenbenzthiazoline-6-sulfonic acid) + H2O2	-	Geobacter sulfurreducens	?	-	?
2,2'-azino-bis(3-ethylenbenzthiazoline-6-sulfonic acid) + H2O2	-	Geobacter sulfurreducens DSM 12127	?	-	?

Subunits

Subunits	Comment	Organism
More	three-dimensional structure of MacA by molecular replacement, model building, overview	Geobacter sulfurreducens

Synonyms

Synonyms	Comment	Organism
MacA	-	Geobacter sulfurreducens

Cofactor

Cofactor	Comment	Organism	Structure
heme	-	Geobacter sulfurreducens

General Information

General Information	Comment	Organism
evolution	MacA belongs to the family of diheme cytochrome c peroxidases	Geobacter sulfurreducens