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Literature summary for 1.11.1.27 extracted from

  • Lim, J.G.; Bang, Y.J.; Choi, S.H.
    Characterization of the Vibrio vulnificus 1-Cys peroxiredoxin Prx3 and regulation of its expression by the Fe-S cluster regulator IscR in response to oxidative stress and iron starvation (2014), J. Biol. Chem., 289, 36263-36274 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.27

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged proteins is expressed in Escherichia coli BL21(DE3) Vibrio vulnificus

Protein Variants

Protein Variants Comment Organism
C48S mutant enzyme does not exhibit activity Vibrio vulnificus

Organism

Organism UniProt Comment Textmining
Vibrio vulnificus ADV89163
-
-
Vibrio vulnificus MO6-24/O ADV89163
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vibrio vulnificus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 glutathione + H2O2
-
 Vibrio vulnificus glutathione disulfide + H2O
-
 ?
2 glutathione + H2O2
-
 Vibrio vulnificus MO6-24/O glutathione disulfide + H2O
-
 ?
glutathione + H2O2 the enzyme is only active when glutaredoxin 3, glutathione, and glutathione reductase are present together as a reducing system Vibrio vulnificus glutathione disulfide + 2 H2O
-
 ?
glutathione + H2O2 the enzyme is only active when glutaredoxin 3, glutathione, and glutathione reductase are present together as a reducing system Vibrio vulnificus MO6-24/O glutathione disulfide + 2 H2O
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Vibrio vulnificus

Synonyms

Synonyms Comment Organism
Prx3
-
 Vibrio vulnificus
Prx3 ambiguous Vibrio vulnificus

Expression

Organism Comment Expression
Vibrio vulnificus the Fe-S cluster regulator IscR activates prx3 expression by sensing reactive oxygen species and iron starvation. IscR controls the prx3 expression by directly binding to the type 2 IscR-binding site centered at positionx0244 from the transcription start site. Biochemical and molecular genetic evidence demonstrates that IscR senses iron starvation as well as ROS and shifts to the clusterless apo-form, which leads to the increase in its own levels in cells and accordingly, the activation of prx3 up
Vibrio vulnificus the Fe-S cluster regulator IscR senses iron starvation as well as reactive oxygen species and shifts to the Fe-S clusterless apo-form, which leads to the increase of cellular IscR and in turn prx3 expression, contributing to the survival and virulence of Vibrio vulnificus during pathogenesis up

General Information

General Information Comment Organism
malfunction the prx3 mutation impairs growth in the medium containing peroxides and reduced virulence in mice Vibrio vulnificus
malfunction the prx3 mutation impairs growth in the medium containing peroxides and reduces virulence in mice Vibrio vulnificus
physiological function Prx3 is essential for survival under oxidative stress and pathogenesis of Vibrio vulnificus Vibrio vulnificus
physiological function Prx3 is essential for survival under oxidative stress and virulence in mice Vibrio vulnificus