Cloned (Comment) | Organism |
---|---|
His-tagged proteins is expressed in Escherichia coli BL21(DE3) | Vibrio vulnificus |
Protein Variants | Comment | Organism |
---|---|---|
C48S | mutant enzyme does not exhibit activity | Vibrio vulnificus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio vulnificus | ADV89163 | - |
- |
Vibrio vulnificus MO6-24/O | ADV89163 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Vibrio vulnificus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 glutathione + H2O2 | - |
Vibrio vulnificus | glutathione disulfide + H2O | - |
? | |
2 glutathione + H2O2 | - |
Vibrio vulnificus MO6-24/O | glutathione disulfide + H2O | - |
? | |
glutathione + H2O2 | the enzyme is only active when glutaredoxin 3, glutathione, and glutathione reductase are present together as a reducing system | Vibrio vulnificus | glutathione disulfide + 2 H2O | - |
? | |
glutathione + H2O2 | the enzyme is only active when glutaredoxin 3, glutathione, and glutathione reductase are present together as a reducing system | Vibrio vulnificus MO6-24/O | glutathione disulfide + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Vibrio vulnificus |
Synonyms | Comment | Organism |
---|---|---|
Prx3 | - |
Vibrio vulnificus |
Prx3 | ambiguous | Vibrio vulnificus |
Organism | Comment | Expression |
---|---|---|
Vibrio vulnificus | the Fe-S cluster regulator IscR activates prx3 expression by sensing reactive oxygen species and iron starvation. IscR controls the prx3 expression by directly binding to the type 2 IscR-binding site centered at positionx0244 from the transcription start site. Biochemical and molecular genetic evidence demonstrates that IscR senses iron starvation as well as ROS and shifts to the clusterless apo-form, which leads to the increase in its own levels in cells and accordingly, the activation of prx3 | up |
Vibrio vulnificus | the Fe-S cluster regulator IscR senses iron starvation as well as reactive oxygen species and shifts to the Fe-S clusterless apo-form, which leads to the increase of cellular IscR and in turn prx3 expression, contributing to the survival and virulence of Vibrio vulnificus during pathogenesis | up |
General Information | Comment | Organism |
---|---|---|
malfunction | the prx3 mutation impairs growth in the medium containing peroxides and reduced virulence in mice | Vibrio vulnificus |
malfunction | the prx3 mutation impairs growth in the medium containing peroxides and reduces virulence in mice | Vibrio vulnificus |
physiological function | Prx3 is essential for survival under oxidative stress and pathogenesis of Vibrio vulnificus | Vibrio vulnificus |
physiological function | Prx3 is essential for survival under oxidative stress and virulence in mice | Vibrio vulnificus |