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Literature summary for 1.11.1.26 extracted from

  • Zhang, B.; Gu, H.; Yang, Y.; Bai, H.; Zhao, C.; Si, M.; Su, T.; Shen, X.
    Molecular mechanisms of AhpC in resistance to oxidative stress in Burkholderia thailandensis (2019), Front. Microbiol., 10, 1483 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.26

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Burkholderia thailandensis

Protein Variants

Protein Variants Comment Organism
C171S/C173S inactive Burkholderia thailandensis
C57S inactive Burkholderia thailandensis
C57S/C171S inactive Burkholderia thailandensis
C57S/C173S inactive Burkholderia thailandensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0692
-
 cumene hydroperoxide in the presence of AhpD as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
0.0978
-
 tert-butyl hydroperoxide in the presence of AhpD as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
0.1473
-
 cumene hydroperoxide in the presence of thioredoxin C as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
0.1934
-
 H2O2 in the presence of AhpD as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
0.2025
-
 tert-butyl hydroperoxide in the presence of thioredoxin C as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
0.3479
-
 H2O2 in the presence of thioredoxin C as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis

Organism

Organism UniProt Comment Textmining
Burkholderia thailandensis Q2SWT3
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Burkholderia thailandensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information AhpD directly interacts with the enzyme as an electron donor, and the conserved Cys residues in active site of AhpD are important for enzyme reduction Burkholderia thailandensis ?
-
-
NADH + cumene hydroperoxide + H+
-
 Burkholderia thailandensis ?
-
 ?
NADH + H2O2 + H+
-
 Burkholderia thailandensis NAD+ + 2 H2O
-
 ?
NADH + peroxynitrite + H+
-
 Burkholderia thailandensis ?
-
 ?
NADH + tert-butyl hydroperoxide + H+
-
 Burkholderia thailandensis ?
-
 ?

Synonyms

Synonyms Comment Organism
AhpC
-
 Burkholderia thailandensis
alkyl hydroperoxide reductase subunit C
-
 Burkholderia thailandensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6
-
 H2O2 in the presence of thioredoxin C as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
7.1
-
 H2O2 in the presence of AhpD as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
9.5
-
 tert-butyl hydroperoxide in the presence of thioredoxin C as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
11.6
-
 cumene hydroperoxide in the presence of thioredoxin C as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
26.1
-
 tert-butyl hydroperoxide in the presence of AhpD as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
29.7
-
 cumene hydroperoxide in the presence of AhpD as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis

General Information

General Information Comment Organism
malfunction enzyme deletion affects the survival rate of Burkholderia thailandensis in response to peroxynitrite Burkholderia thailandensis
metabolism the enzyme plays an important role in peroxides and peroxynitrite damage response Burkholderia thailandensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10
-
 H2O2 in the presence of thioredoxin C as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
36
-
 H2O2 in the presence of AhpD as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
47
-
 tert-butyl hydroperoxide in the presence of thioredoxin C as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
79
-
 cumene hydroperoxide in the presence of thioredoxin C as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
267
-
 tert-butyl hydroperoxide in the presence of AhpD as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
429
-
 cumene hydroperoxide in the presence of AhpD as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis