Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.11.1.24 extracted from

  • Parsonage, D.; Desrosiers, D.C.; Hazlett, K.R.; Sun, Y.; Nelson, K.J.; Cox, D.L.; Radolf, J.D.; Poole, L.B.
    Broad specificity AhpC-like peroxiredoxin and its thioredoxin reductant in the sparse antioxidant defense system of Treponema pallidum (2010), Proc. Natl. Acad. Sci. USA, 107, 6240-6245.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.24

Inhibitors

Inhibitors Comment Organism Structure
additional information TpAhpC is relatively resistant to inactivation during turnover with hydroperoxide substrates Treponema pallidum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0096
-
 cumene hydroperoxide pH 7.0, 25°C, TpAhpC Treponema pallidum
0.012
-
 H2O2 pH 7.0, 25°C, TpAhpC Treponema pallidum
0.0388
-
 thioredoxin pH 7.0, 25°C, TpAhpC with cumene hydroperoxide Treponema pallidum
0.062
-
 thioredoxin pH 7.0, 25°C, TpAhpC with H2O2 Treponema pallidum
0.0794
-
 thioredoxin pH 7.0, 25°C, TpAhpC with t-butyl hydroperoxide Treponema pallidum
0.104
-
 t-butyl hydroperoxide pH 7.0, 25°C, TpAhpC Treponema pallidum

Organism

Organism UniProt Comment Textmining
Treponema pallidum O83522 gene TP0509
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 thioredoxin + cumene hydroperoxide
-
 Treponema pallidum thioredoxin disulfide + H2O + 2-phenylpropan-2-ol
-
 ?
2 thioredoxin + H2O2
-
 Treponema pallidum thioredoxin disulfide + 2 H2O
-
 ?
2 thioredoxin + t-butyl hydroperoxide
-
 Treponema pallidum thioredoxin disulfide + H2O + t-butanol
-
 ?
additional information TpAhpC is a broad specificity peroxiredoxin, substrate specificity, overview Treponema pallidum ?
-
 ?

Subunits

Subunits Comment Organism
More reduced TpAhpC forms stable octamers or decamers in solution whereas oxidation generates a heterogeneous mixture of oligomeric species. TpAhpC undergoes redox-sensitive oligomer formation Treponema pallidum

Synonyms

Synonyms Comment Organism
AhpC-like peroxiredoxin
-
 Treponema pallidum
AhpC-like Prx
-
 Treponema pallidum
alkyl hydroperoxide reductase
-
 Treponema pallidum
More the enzyme belongs to the peroxiredoxin enzyme family Treponema pallidum
TP0509
-
 Treponema pallidum
typical 2-Cys Prx
-
 Treponema pallidum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Treponema pallidum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.8
-
 cumene hydroperoxide pH 7.0, 25°C, TpAhpC Treponema pallidum
9.8
-
 thioredoxin pH 7.0, 25°C, TpAhpC with cumene hydroperoxide Treponema pallidum
17
-
 H2O2 pH 7.0, 25°C, TpAhpC Treponema pallidum
17
-
 thioredoxin pH 7.0, 25°C, TpAhpC with H2O2 Treponema pallidum
20.9
-
 t-butyl hydroperoxide pH 7.0, 25°C, TpAhpC Treponema pallidum
20.9
-
 thioredoxin pH 7.0, 25°C, TpAhpC with t-butyl hydroperoxide Treponema pallidum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Treponema pallidum

General Information

General Information Comment Organism
additional information although this Tp peroxiredoxin closely resembles AhpC-like peroxidoxins, Treponema pallidum lacks AhpF, the typical reductant for such enzymes. Functionally, TpAhpC resembles largely eukaryotic, nonAhpC typical 2-Cys Prx proteins in using thioredoxin as an efficient electron donor and exhibiting broad specificity toward hydroperoxide substrates Treponema pallidum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
200
-
 t-butyl hydroperoxide pH 7.0, 25°C, TpAhpC Treponema pallidum
250
-
 thioredoxin pH 7.0, 25°C, TpAhpC with cumene hydroperoxide Treponema pallidum
260
-
 thioredoxin pH 7.0, 25°C, TpAhpC with t-butyl hydroperoxide Treponema pallidum
270
-
 thioredoxin pH 7.0, 25°C, TpAhpC with H2O2 Treponema pallidum
1000
-
 cumene hydroperoxide pH 7.0, 25°C, TpAhpC Treponema pallidum
1400
-
 H2O2 pH 7.0, 25°C, TpAhpC Treponema pallidum