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Literature summary for 1.11.1.24 extracted from

  • Horta, B.B.; de Oliveira, M.A.; Discola, K.F.; Cussiol, J.R.; Netto, L.E.
    Structural and biochemical characterization of peroxiredoxin Qbeta from Xylella fastidiosa: catalytic mechanism and high reactivity (2010), J. Biol. Chem., 285, 16051-16065.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.24

Cloned(Commentary)

Cloned (Comment) Organism
gene prxq, expression of wild-type and mutant PrxQs in Escherichia coli strain BL21(DE3) Xylella fastidiosa

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant PrxQ C47S, sitting drop vapor diffusion method, 5-15 mg/ml protein in 5 mM sodium citrate, pH 5.0, and 10 mM DTT, are mixed with reservoir solution containing 0.1 M HEPES-HCl, pH 7.5, and 20% PEG 8000 at 20°C, X-ray diffraction structure determination and analysis Xylella fastidiosa

Protein Variants

Protein Variants Comment Organism
C101S site-directed mutagenesis, structure comparison to the wild-type enzyme Xylella fastidiosa
C23S site-directed mutagenesis, structure comparison to the wild-type enzyme Xylella fastidiosa
C47S site-directed mutagenesis, structure comparison to the wild-type enzyme Xylella fastidiosa
C83S site-directed mutagenesis, structure comparison to the wild-type enzyme, dimedone likely inactivated the XfPrxQ C83S protein because sulfenic acids persist long enough to react with dimedone only in the absence of Cys83 Xylella fastidiosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
 H2O2 pH 7.4, 37°C, recombinant enzyme Xylella fastidiosa
0.037
-
 cumene hydroperoxide pH 7.4, 37°C, recombinant enzyme Xylella fastidiosa
0.072
-
 t-butyl hydroperoxide pH 7.4, 37°C, recombinant enzyme Xylella fastidiosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17813
-
 x * 17813, sequence calculation Xylella fastidiosa

Organism

Organism UniProt Comment Textmining
Xylella fastidiosa Q9PER7 gene prxq or bcp
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.67
-
 pH 7.4, 37°C, recombinant enzyme, substarte t-butyl hydroperoxide Xylella fastidiosa
1.49
-
 pH 7.4, 37°C, recombinant enzyme, substrate cumene hydroperoxide Xylella fastidiosa
1.76
-
 pH 7.4, 37°C, recombinant enzyme, substrate H2O2 Xylella fastidiosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 thioredoxin + cumene hydroperoxide
-
 Xylella fastidiosa thioredoxin disulfide + H2O + 2-phenylpropan-2-ol
-
 ?
2 thioredoxin + H2O2
-
 Xylella fastidiosa thioredoxin disulfide + 2 H2O
-
 ?
2 thioredoxin + t-butyl hydroperoxide
-
 Xylella fastidiosa thioredoxin disulfide + H2O + t-butanol
-
 ?
additional information Cys83 is the resolving cysteine of XfPrxQ Xylella fastidiosa ?
-
 ?

Subunits

Subunits Comment Organism
? x * 17813, sequence calculation Xylella fastidiosa
More primary structure and peptide mapping for detection of the disulfide-containing peptide, using chemical modification and tryptic digestion with HPLC-ESI-MS analyses, overview. Conformational analysis of XfPrxQ, modelling, overview Xylella fastidiosa

Synonyms

Synonyms Comment Organism
bacterioferritin comigratory protein
-
 Xylella fastidiosa
BCP
-
 Xylella fastidiosa
More peroxiredoxin PrxQ belongs to the subdivision of the bacterioferritin comigratory protein family of bacterial peroxiredoxins Xylella fastidiosa
PrxQ
-
 Xylella fastidiosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Xylella fastidiosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.41
-
 cumene hydroperoxide pH 7.4, 37°C, recombinant enzyme Xylella fastidiosa
1.1
-
 H2O2 pH 7.4, 37°C, recombinant enzyme Xylella fastidiosa
2
-
 t-butyl hydroperoxide pH 7.4, 37°C, recombinant enzyme Xylella fastidiosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Xylella fastidiosa

General Information

General Information Comment Organism
physiological function to survive under oxidative stress imposed by the host, microorganisms express antioxidant proteins, including cysteine-based peroxidases named peroxiredoxins Xylella fastidiosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.49
-
 cumene hydroperoxide pH 7.4, 37°C, recombinant enzyme Xylella fastidiosa
33.5
-
 H2O2 pH 7.4, 37°C, recombinant enzyme Xylella fastidiosa
53.4
-
 t-butyl hydroperoxide pH 7.4, 37°C, recombinant enzyme Xylella fastidiosa