Cloned (Comment) | Organism |
---|---|
gene prx4, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, expression of enzyme versions PRX264, Prx231, and Prx197 in Escherichia coli strain BL21(DE3) | Seriola lalandi |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of shorter versions of the enzyme, Prx231 and Prx197, both exhibit thioredoxin-dependent peroxidase activity, whereas fill-length Prx264 does not | Seriola lalandi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | both active forms of the enzyme displayed a single-displacement reaction mechanism and typical saturable Michaelis-Menten type kinetics | Seriola lalandi | |
0.00098 | - |
H2O2 | pH 7.0, temperature not specified in the publication, Prx231 | Seriola lalandi | |
0.0032 | - |
H2O2 | pH 7.0, temperature not specified in the publication, Prx197 | Seriola lalandi | |
0.152 | - |
cumene hydroperoxide | pH 7.0, temperature not specified in the publication, Prx231 | Seriola lalandi | |
0.18 | - |
t-butyl hydroperoxide | pH 7.0, temperature not specified in the publication, Prx231 | Seriola lalandi | |
0.191 | - |
cumene hydroperoxide | pH 7.0, temperature not specified in the publication, Prx197 | Seriola lalandi | |
0.193 | - |
t-butyl hydroperoxide | pH 7.0, temperature not specified in the publication, Prx197 | Seriola lalandi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 thioredoxin + ROOH | Seriola lalandi | - |
thioredoxin disulfide + H2O + ROH | - |
? | |
additional information | Seriola lalandi | shorter versions of the enzyme, Prx231 and Prx197, both exhibit thioredoxin-dependent peroxidase activity, whereas fill-length Prx264 does not | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Seriola lalandi | B9USM4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme versions PRX264, Prx231, and Prx197 from Escherichia coli strain BL21(DE3) | Seriola lalandi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 thioredoxin + cumene hydroperoxide | - |
Seriola lalandi | thioredoxin disulfide + H2O + 2-phenylpropan-2-ol | - |
? | |
2 thioredoxin + H2O2 | - |
Seriola lalandi | thioredoxin disulfide + 2 H2O | - |
? | |
2 thioredoxin + ROOH | - |
Seriola lalandi | thioredoxin disulfide + H2O + ROH | - |
? | |
2 thioredoxin + t-butyl hydroperoxide | - |
Seriola lalandi | thioredoxin disulfide + H2O + t-butanol | - |
? | |
additional information | shorter versions of the enzyme, Prx231 and Prx197, both exhibit thioredoxin-dependent peroxidase activity, whereas fill-length Prx264 does not | Seriola lalandi | ? | - |
? | |
additional information | both active forms of the enzyme displayed a single-displacement reaction mechanism and typical saturable Michaelis-Menten type kinetics | Seriola lalandi | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-Cys peroxiredoxin 4 | - |
Seriola lalandi |
peroxiredoxin 4 | - |
Seriola lalandi |
Prx 4 | - |
Seriola lalandi |
typical 2-Cys Prx | - |
Seriola lalandi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.2 | - |
t-butyl hydroperoxide | pH 7.0, temperature not specified in the publication, Prx197 | Seriola lalandi | |
7.3 | - |
cumene hydroperoxide | pH 7.0, temperature not specified in the publication, Prx197 | Seriola lalandi | |
10.4 | - |
t-butyl hydroperoxide | pH 7.0, temperature not specified in the publication, Prx231 | Seriola lalandi | |
15.2 | - |
cumene hydroperoxide | pH 7.0, temperature not specified in the publication, Prx231 | Seriola lalandi | |
22.3 | - |
H2O2 | pH 7.0, temperature not specified in the publication, Prx197 | Seriola lalandi | |
24.6 | - |
H2O2 | pH 7.0, temperature not specified in the publication, Prx231 | Seriola lalandi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
50 | - |
cumene hydroperoxide | pH 7.0, temperature not specified in the publication, Prx231 | Seriola lalandi | |
160 | - |
t-butyl hydroperoxide | pH 7.0, temperature not specified in the publication, Prx197 | Seriola lalandi | |
380 | - |
cumene hydroperoxide | pH 7.0, temperature not specified in the publication, Prx197 | Seriola lalandi | |
580 | - |
t-butyl hydroperoxide | pH 7.0, temperature not specified in the publication, Prx231 | Seriola lalandi | |
710 | - |
H2O2 | pH 7.0, temperature not specified in the publication, Prx197 | Seriola lalandi | |
2500 | - |
H2O2 | pH 7.0, temperature not specified in the publication, Prx231 | Seriola lalandi |