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Literature summary for 1.11.1.24 extracted from

  • Wakita, M.; Masuda, S.; Motohashi, K.; Hisabori, T.; Ohta, H.; Takamiya, K.
    The significance of type II and PrxQ peroxiredoxins for antioxidative stress response in the purple bacterium Rhodobacter sphaeroides (2007), J. Biol. Chem., 282, 27792-27801.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
chitin-tagged protein is expressed in Escherichia coli BL21(DE3) cells Cereibacter sphaeroides

Protein Variants

Protein Variants Comment Organism
C44S inactive Cereibacter sphaeroides
C80S reduced activity Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00475
-
H2O2 PrxT, in 50 mM HEPES/NaOH, pH 7.3, at 25°C Cereibacter sphaeroides
0.00665
-
cumene hydroperoxide PrxT, in 50 mM HEPES/NaOH, pH 7.3, at 25°C Cereibacter sphaeroides
0.00848
-
cumene hydroperoxide PrxQ, in 50 mM HEPES/NaOH, pH 7.3, at 25°C Cereibacter sphaeroides
0.00856
-
H2O2 PrxQ, in 50 mM HEPES/NaOH, pH 7.3, at 25°C Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
with chitin beads Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cumene hydroperoxide + reduced thioredoxin
-
Cereibacter sphaeroides 2-phenylpropan-2-ol + oxidized thioredoxin + H2O
-
?
H2O2 + reduced thioredoxin
-
Cereibacter sphaeroides H2O + oxidized thioredoxin
-
?

Synonyms

Synonyms Comment Organism
PrxQ
-
Cereibacter sphaeroides
PrxT
-
Cereibacter sphaeroides
type II peroxiredoxin
-
Cereibacter sphaeroides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.274
-
cumene hydroperoxide PrxT, in 50 mM HEPES/NaOH, pH 7.3, at 25°C Cereibacter sphaeroides
0.281
-
cumene hydroperoxide PrxQ, in 50 mM HEPES/NaOH, pH 7.3, at 25°C Cereibacter sphaeroides
0.285
-
H2O2 PrxQ, in 50 mM HEPES/NaOH, pH 7.3, at 25°C Cereibacter sphaeroides
0.285
-
H2O2 PrxT, in 50 mM HEPES/NaOH, pH 7.3, at 25°C Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
thioredoxin dependent Cereibacter sphaeroides