Any feedback?
Literature summary for 1.11.1.24 extracted from
- Cloning and characterization of Arenicola marina peroxiredoxin 6, an annelid two-cysteine peroxiredoxin highly homologous to mammalian one-cysteine peroxiredoxins (2008), Free Radic. Biol. Med., 45, 482-493.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Arenicola marina |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C183S | strongly reduced activity | Arenicola marina |
| C45S | inactive | Arenicola marina |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
1 * 22000, SDS-PAGE, under atmospheric oxidations the protein appears as a small oxidized monomer and as a major oxidized dimer | Arenicola marina |
| 25000 | - |
1 * 25000, SDS-PAGE, after reduction with 10 mM dithiothreitol the protein appears as a reduced monomer | Arenicola marina |
| 50000 | - |
2 * 50000, SDS-PAGE, under atmospheric oxidations the protein appears as a small oxidized monomer and as a major oxidized dimer | Arenicola marina |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arenicola marina | Q1AN22 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+ column chromatography | Arenicola marina |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| body wall | - |
Arenicola marina | - |
| gill | - |
Arenicola marina | - |
| additional information | no activity is detected in muscles, coelomic fluid, and other internal structures | Arenicola marina | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cumene hydroperoxide + reduced thioredoxin | - |
Arenicola marina | 2-phenylpropan-2-ol + oxidized thioredoxin + H2O | - |
? |  |
| tert-butyl hydroperoxide + reduced dithiothreitol | - |
Arenicola marina | tert-butanol + oxidized dithiothreitol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 50000, SDS-PAGE, under atmospheric oxidations the protein appears as a small oxidized monomer and as a major oxidized dimer | Arenicola marina |
| monomer | 1 * 22000, SDS-PAGE, under atmospheric oxidations the protein appears as a small oxidized monomer and as a major oxidized dimer | Arenicola marina |
| monomer | 1 * 25000, SDS-PAGE, after reduction with 10 mM dithiothreitol the protein appears as a reduced monomer | Arenicola marina |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| peroxiredoxin 6 | - |
Arenicola marina |
| Prdx6 | classified as a 2-Cys PRDX | Arenicola marina |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thioredoxin | - |
Arenicola marina | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
