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Literature summary for 1.11.1.24 extracted from
- Peroxiredoxins as cellular guardians in Sulfolobus solfataricus: characterization of Bcp1, Bcp3 and Bcp4 (2008), FEBS J., 275, 2067-2077.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Saccharolobus solfataricus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16980 | - |
Bcp3, calculated from amino acid sequence | Saccharolobus solfataricus |
| 17460 | - |
Bcp1, calculated from amino acid sequence | Saccharolobus solfataricus |
| 17460 | - |
Bcp4, calculated from amino acid sequence | Saccharolobus solfataricus |
| 18000 | - |
Bcp1, SDS-PAGE | Saccharolobus solfataricus |
| 18040 | - |
Bcp3, MALDI-TOF mass spectrometry | Saccharolobus solfataricus |
| 18530 | - |
Bcp1, MALDI-TOF mass spectrometry | Saccharolobus solfataricus |
| 18530 | - |
Bcp4, MALDI-TOF mass spectrometry | Saccharolobus solfataricus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| urea | Bcp4 retains 50% of its activity after a 30 min incubation in 6 M urea | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | Q97VL0 | - |
- |
| Saccharolobus solfataricus | Q97WG5 | - |
- |
| Saccharolobus solfataricus | Q97WP9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap HP column chromatography, Resource S column chromatography, and Resource Q column chromatography | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2O2 + reduced thioredoxin | H2O2 is the preferred substrate compared to tert-butyl hydroperoxide | Saccharolobus solfataricus | H2O + oxidized thioredoxin | - |
? |  |
| tert-butyl hydroperoxide + reduced thioredoxin | H2O2 is the preferred substrate compared to tert-butyl hydroperoxide | Saccharolobus solfataricus | tert-butanol + oxidized thioredoxin + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | Bcp1, gel filtration | Saccharolobus solfataricus |
| dimer | Bcp3, gel filtration | Saccharolobus solfataricus |
| monomer | Bcp4, gel filtration | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bacterioferritin comigratory protein | - |
Saccharolobus solfataricus |
| Bcp1 | the isozyme is classified as a 2-Cys peroxiredoxin | Saccharolobus solfataricus |
| Bcp3 | the isozyme is classified as a 1-Cys peroxiredoxin | Saccharolobus solfataricus |
| Bcp4 | the isozyme is classified as a 2-Cys peroxiredoxin | Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Saccharolobus solfataricus |
| 85 | - |
Bcp1 and Bcp3 | Saccharolobus solfataricus |
| 95 | 100 | - |
Saccharolobus solfataricus |
| 95 | 100 | Bcp4 | Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | 100 | Bcp1 shows no loss of activity after 2 h incubation at 80°C, displays a half-life of 2 h at 95°C but after a 60 min incubation at 100°C its relative activity drops to 20% of the starting value | Saccharolobus solfataricus |
| 80 | 100 | Bcp3 shows no loss of activity after 2 h incubation at 80°C, after 2 h at 95°C relative activity drops to 60% | Saccharolobus solfataricus |
| 80 | 100 | Bcp4 shows no loss of activity after 2 h incubation at 80°C, after 2 h at 95°C relative activity drops to 92% | Saccharolobus solfataricus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thioredoxin | - |
Saccharolobus solfataricus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Saccharolobus solfataricus | Bcp4, calculated from amino acid sequence | - |
7.65 |
| Saccharolobus solfataricus | Bcp1, calculated from amino acid sequence | - |
7.73 |
| Saccharolobus solfataricus | Bcp3, calculated from amino acid sequence | - |
8.84 |
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