Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Taiwanofungus camphoratus |
General Stability | Organism |
---|---|
1.6 M imidazole seems to promote protein degradation | Taiwanofungus camphoratus |
sensitive to trypsin and chymotrypsin treatment. Very little or no activity detected after 40 min incubation with either protease | Taiwanofungus camphoratus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
imidazole | approximately 70% of the Prx activity is lost in the presence of 0.4 M imidazole or higher. The presence of 1.6 M imidazole seems to promote protein degradation | Taiwanofungus camphoratus | |
SDS | activity is lost completely in the presence of 1% SDS or higher | Taiwanofungus camphoratus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
x * 25000, SDS-PAGE | Taiwanofungus camphoratus |
25081 | - |
x * 25081, calculated from sequence | Taiwanofungus camphoratus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Taiwanofungus camphoratus | A1KZ88 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Taiwanofungus camphoratus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit body | - |
Taiwanofungus camphoratus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tert-butyl hydroperoxide + reduced dithiothreitol | - |
Taiwanofungus camphoratus | tert-butanol + oxidized dithiothreitol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, SDS-PAGE | Taiwanofungus camphoratus |
? | x * 25081, calculated from sequence | Taiwanofungus camphoratus |
Synonyms | Comment | Organism |
---|---|---|
1-Cys peroxiredoxin | - |
Taiwanofungus camphoratus |
Ac-1-Cys Prx | - |
Taiwanofungus camphoratus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half-life: 15.5 min | Taiwanofungus camphoratus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Taiwanofungus camphoratus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.4 | 9 | no activity at or below pH 5.4, optimal activity at pH 9.0 | Taiwanofungus camphoratus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.8 | 10.2 | 37°C, 30 min, stable | Taiwanofungus camphoratus |