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Literature summary for 1.11.1.21 extracted from

  • Purka, P.; Ihsanawat, I.; Natalia, D.; Syah, Y.; Retnoningrum, D.; Kusuma, H.
    Mutation of katG in a clinical isolate of Mycobacterium tuberculosis Effects on catalase-peroxidase for isoniazid activation (2016), Ukr. Biochem. J., 88, 71-81 .
No PubMed abstract available
Search for more literature for 1.11.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
N238S the catalytic efficiency (Kcat/KM) of the mutant decreases to 41 and 52% for catalase and peroxidase, respectively, compared to the wild type enzyme Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 o-Dianisidine wild type enzyme, at pH 4.5 and 30°C Mycobacterium tuberculosis
0.35
-
 o-Dianisidine mutant enzyme N238S, at pH 4.5 and 30°C Mycobacterium tuberculosis
3.16
-
 H2O2 wild type enzyme, at pH 7.0 and 30°C Mycobacterium tuberculosis
4.44
-
 H2O2 mutant enzyme N238S, at pH 7.0 and 30°C Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WIE5
-
-
Mycobacterium tuberculosis H37Rv P9WIE5
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 H2O2
-
 Mycobacterium tuberculosis O2 + H2O
-
 ?
2 H2O2
-
 Mycobacterium tuberculosis H37Rv O2 + H2O
-
 ?
isoniazid + phenol + H2O2
-
 Mycobacterium tuberculosis benzoquinone + ?
-
 ?
isoniazid + phenol + H2O2
-
 Mycobacterium tuberculosis H37Rv benzoquinone + ?
-
 ?
o-dianisidine + tert-butyl hydroperoxide + H2O2
-
 Mycobacterium tuberculosis o-dianisidin quinone diimine + ?
-
 ?
o-dianisidine + tert-butyl hydroperoxide + H2O2
-
 Mycobacterium tuberculosis H37Rv o-dianisidin quinone diimine + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
KatG
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
33.3
-
 o-Dianisidine mutant enzyme N238S, at pH 4.5 and 30°C Mycobacterium tuberculosis
49
-
 o-Dianisidine wild type enzyme, at pH 4.5 and 30°C Mycobacterium tuberculosis
227.5
-
 H2O2 mutant enzyme N238S, at pH 7.0 and 30°C Mycobacterium tuberculosis
272.3
-
 H2O2 wild type enzyme, at pH 7.0 and 30°C Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
51.2
-
 H2O2 mutant enzyme N238S, at pH 7.0 and 30°C Mycobacterium tuberculosis
86.2
-
 H2O2 wild type enzyme, at pH 7.0 and 30°C Mycobacterium tuberculosis
96
-
 o-Dianisidine mutant enzyme N238S, at pH 4.5 and 30°C Mycobacterium tuberculosis
199
-
 o-Dianisidine wild type enzyme, at pH 4.5 and 30°C Mycobacterium tuberculosis