Literature summary for 1.11.1.21 extracted from

Kamachi, S.; Hirabayashi, K.; Tamoi, M.; Shigeoka, S.; Tada, T.; Wada, K.
The crystal structure of isoniazid-bound KatG catalase-peroxidase from Synechococcuselongatus PCC7942 (2015), FEBS J., 282, 54-64 .

Search for more literature for 1.11.1.21

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)pLysS cells	Synechococcus elongatus

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with isoniazid, sitting drop vapor diffusion method, using 4.3 M sodium formate and 100 mM sodium citrate, pH 6.4	Synechococcus elongatus

Protein Variants

Protein Variants	Comment	Organism
S308T	the mutant shows strongly reduced activity compared to the wild type enzyme	Synechococcus elongatus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
5.6	-	isoniazid	wild type enzyme, at pH 7.8 and 37°C	Synechococcus elongatus
10.18	-	isoniazid	mutant enzyme S308T, at pH 7.8 and 37°C	Synechococcus elongatus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2 H2O2	Synechococcus elongatus	-	O2 + H2O	-	?
2 H2O2	Synechococcus elongatus PCC7942	-	O2 + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Synechococcus elongatus	Q31MN3	-	-
Synechococcus elongatus PCC7942	Q31MN3	-	-

Purification (Commentary)

Purification (Comment)	Organism
Q Sepharose column chromatography and Mono Q column chromatography	Synechococcus elongatus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 H2O2	-	Synechococcus elongatus	O2 + H2O	-	?
2 H2O2	-	Synechococcus elongatus PCC7942	O2 + H2O	-	?
isoniazid + H2O2	-	Synechococcus elongatus	oxidized isoniazid + H2O	-	?
isoniazid + H2O2	-	Synechococcus elongatus PCC7942	oxidized isoniazid + H2O	-	?

Synonyms

Synonyms	Comment	Organism
KatG	-	Synechococcus elongatus

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Release 2023.1 (January 2023)