Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
E291A | the mutant shows 89% peroxidase activity and 139% catalase activity compared to the wild type enzyme | Mycobacterium tuberculosis |
E291K | the mutant shows 200% peroxidase activity and 79% catalase activity compared to the wild type enzyme | Mycobacterium tuberculosis |
E291K/E292A | the mutant shows 222% peroxidase activity and 102% catalase activity compared to the wild type enzyme | Mycobacterium tuberculosis |
I290A | the mutant shows 31% peroxidase activity and 93% catalase activity compared to the wild type enzyme | Mycobacterium tuberculosis |
I290A/Q293A | the mutant shows 56% peroxidase activity and 64% catalase activity compared to the wild type enzyme | Mycobacterium tuberculosis |
I290V | the mutant shows 35% peroxidase activity and 67% catalase activity compared to the wild type enzyme | Mycobacterium tuberculosis |
I290V/Q293V | the mutant shows 44% peroxidase activity and 47% catalase activity compared to the wild type enzyme | Mycobacterium tuberculosis |
Q293A | the mutant shows 59% peroxidase activity and 102% catalase activity compared to the wild type enzyme | Mycobacterium tuberculosis |
Q293E | the mutant shows 62% peroxidase activity and 80% catalase activity compared to the wild type enzyme | Mycobacterium tuberculosis |
Q293V | the mutant shows 25% peroxidase activity and 44% catalase activity compared to the wild type enzyme | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme E291K, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
0.028 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme E291K/E292A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
0.037 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme I290A/Q293A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
0.05 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme Q293E, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
0.06 | - |
H2O2 | mutant enzyme E291K, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
0.06 | - |
H2O2 | mutant enzyme E291K/E292A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
0.07 | - |
H2O2 | mutant enzyme I290A/Q293A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
0.071 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme E291A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
0.078 | - |
H2O2 | mutant enzyme Q293A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
0.08 | - |
H2O2 | mutant enzyme I290V, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
0.08 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme Q293A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
0.081 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme I290V/Q293V, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
0.087 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | wild type enzyme, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
0.089 | - |
H2O2 | mutant enzyme Q293E, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
0.09 | - |
H2O2 | mutant enzyme I290A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
0.09 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme Q293V, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
0.1 | - |
H2O2 | mutant enzyme E291A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
0.13 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme I290V, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
0.16 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme I290A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
0.21 | - |
H2O2 | mutant enzyme I290V/Q293V, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
0.31 | - |
H2O2 | wild type enzyme, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
0.5 | - |
H2O2 | mutant enzyme Q293V, at pH 5.0 and 23°C | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Mycobacterium tuberculosis | oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KatG | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.3 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme I290A/Q293A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
13.6 | - |
H2O2 | mutant enzyme I290A/Q293A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
15.2 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme Q293V, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
20 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme Q293E, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
22.5 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme I290V/Q293V, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
23.8 | - |
H2O2 | mutant enzyme Q293E, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
24 | - |
H2O2 | mutant enzyme I290V/Q293V, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
26 | - |
H2O2 | mutant enzyme I290A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
26 | - |
H2O2 | mutant enzyme I290V, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
26 | - |
H2O2 | mutant enzyme Q293V, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
27.8 | - |
H2O2 | mutant enzyme Q293A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
28.5 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme I290V, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
29.3 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme Q293A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
29.6 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme E291K, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
32.5 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme I290A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
34 | - |
H2O2 | mutant enzyme E291K, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
35.3 | - |
H2O2 | mutant enzyme E291K/E292A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
38 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme E291K/E292A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
39.7 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme E291A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
40 | - |
H2O2 | mutant enzyme E291A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
55 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | wild type enzyme, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
83 | - |
H2O2 | wild type enzyme, at pH 5.0 and 23°C | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
49 | - |
H2O2 | mutant enzyme Q293V, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
110 | - |
H2O2 | mutant enzyme I290V/Q293V, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
140 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme E291K/E292A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
160 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme Q293V, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
200 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme I290A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
210 | - |
H2O2 | mutant enzyme I290A/Q293A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
220 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme I290V, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
268 | - |
H2O2 | mutant enzyme Q293E, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
270 | - |
H2O2 | wild type enzyme, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
280 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme I290V/Q293V, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
310 | - |
H2O2 | mutant enzyme I290A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
350 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme I290A/Q293A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
350 | - |
H2O2 | mutant enzyme I290V, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
370 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme Q293A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
380 | - |
H2O2 | mutant enzyme Q293A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
390 | - |
H2O2 | mutant enzyme E291A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
390 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme Q293E, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
560 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme E291A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
600 | - |
H2O2 | mutant enzyme E291K, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
620 | - |
H2O2 | mutant enzyme E291K/E292A, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
630 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | wild type enzyme, at pH 5.0 and 23°C | Mycobacterium tuberculosis | |
1260 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme E291K, at pH 5.0 and 23°C | Mycobacterium tuberculosis |