Application | Comment | Organism |
---|---|---|
synthesis | biocatalytic method for stereoselective oxidation of beta-lactams, such as penicillin-G, penicillin-V and cephalosporin-G to their (R)-sulfoxides | Bacillus pumilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydroxylamine | 0.05 mM, 80% inhibition of peroxidase activity, 88% inhibition of catalyse activity | Bacillus pumilus | |
KCN | 0.05 mM, 30% inhibition of peroxidase activity, 22% inhibition of catalyse activity | Bacillus pumilus | |
Sodium azide | 25 mM, 30% inhibition of peroxidase activity, 100% inhibition of catalyse activity | Bacillus pumilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.82 | - |
H2O2 | catalase activity, pH 7.0, 37°C | Bacillus pumilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
gel filtration | Bacillus pumilus |
62140 | - |
1 * 62400, SDS-PAGE, 1 * 62140, MALDI-TOF | Bacillus pumilus |
62400 | - |
1 * 62400, SDS-PAGE, 1 * 62140, MALDI-TOF | Bacillus pumilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus pumilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus pumilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
743 | - |
peroxidase activity, pH 7.0, 37°C | Bacillus pumilus |
818 | - |
catalase activity, pH 7.0, 37°C | Bacillus pumilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 H2O2 | - |
Bacillus pumilus | O2 + 2 H2O | - |
? | |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Bacillus pumilus | ? + H2O | - |
? | |
penicillin G + H2O2 | - |
Bacillus pumilus | penicillin G (R)-sulfoxide + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 62400, SDS-PAGE, 1 * 62140, MALDI-TOF | Bacillus pumilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bacillus pumilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
sharp decrease in activity above | Bacillus pumilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bacillus pumilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | more than 75% of maximum activity within | Bacillus pumilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Bacillus pumilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1300 | - |
H2O2 | catalase activity, pH 7.0, 37°C | Bacillus pumilus |