Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli UM2 cells | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
S315T | the mutant is a competent catalase-peroxidase with reduced activity toward isoniazid. The catalase activity is reduced 6fold and the peroxidase activity is decreased less than 2fold compared with the activities for the wild type enzyme | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
H2O2 | mutant enzyme S315T, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis | |
0.07 | - |
H2O2 | wild type enzyme, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis | |
0.08 | - |
isoniazid | mutant enzyme S315T, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis | |
0.43 | - |
isoniazid | wild type enzyme, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography, Sephacryl S300 gel filtration, and MonoQ HR5/5 column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | - |
Mycobacterium tuberculosis | O2 + H2O | - |
? | |
isoniazid + H2O2 | - |
Mycobacterium tuberculosis | oxidized isoniazid + H2O | - |
? | |
o-dianisidine + tert-butyl hydroperoxide | - |
Mycobacterium tuberculosis | o-dianisidine quinonediimine + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KatG | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.057 | - |
isoniazid | wild type enzyme, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis | |
0.081 | - |
isoniazid | mutant enzyme S315T, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis | |
1.2 | - |
o-Dianisidine | mutant enzyme S315T, peroxidase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis | |
2 | - |
o-Dianisidine | wild type enzyme, peroxidase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis | |
2100 | - |
H2O2 | mutant enzyme S315T, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis | |
5800 | - |
H2O2 | wild type enzyme, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | 1.1 hemes per subunit for wild type KatG | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19 | - |
o-Dianisidine | mutant enzyme S315T, peroxidase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis | |
32 | - |
o-Dianisidine | wild type enzyme, peroxidase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis | |
160 | - |
H2O2 | mutant enzyme S315T, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis | |
1000 | - |
H2O2 | wild type enzyme, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C | Mycobacterium tuberculosis |