Cloned (Comment) | Organism |
---|---|
mutant KatGDELTAFG is expressed in Escherichia coli BL-21 (DE3) pLysS cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the heme environment of mutant KatGDELTAFG (lacking its FG insertion) is highly similar to wild type KatG, but the variant retains only 0.2% catalase activity. In contrast, the deletion reduces peroxidase activity by only 50% | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase activity, mutant enzyme KatGDELTAFG, in 50 mM acetate buffer pH 5.0, at 23°C | Escherichia coli | |
0.23 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase activity, wild type enzyme, in 50 mM acetate buffer pH 5.0, at 23°C | Escherichia coli | |
0.83 | - |
H2O2 | peroxidase activity, wild type enzyme, in 50 mM acetate buffer pH 5.0, at 23°C | Escherichia coli | |
1.5 | - |
H2O2 | peroxidase activity, mutant enzyme KatGDELTAFG, in 50 mM acetate buffer pH 5.0, at 23°C | Escherichia coli | |
4 | - |
H2O2 | catalase activity, wild type enzyme, 100 mM phosphate buffer pH 7.0, at 23°C | Escherichia coli | |
4.5 | - |
H2O2 | catalase activity, mutant enzyme KatGDELTAFG, 100 mM phosphate buffer pH 7.0, at 23°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Escherichia coli | oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? | |
H2O2 | - |
Escherichia coli | O2 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KatG | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26 | - |
H2O2 | catalase activity, mutant enzyme KatGDELTAFG, 100 mM phosphate buffer pH 7.0, at 23°C | Escherichia coli | |
26 | - |
H2O2 | peroxidase activity, mutant enzyme KatGDELTAFG, in 50 mM acetate buffer pH 5.0, at 23°C | Escherichia coli | |
26 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase activity, mutant enzyme KatGDELTAFG, in 50 mM acetate buffer pH 5.0, at 23°C | Escherichia coli | |
52 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase activity, wild type enzyme, in 50 mM acetate buffer pH 5.0, at 23°C | Escherichia coli | |
58 | - |
H2O2 | peroxidase activity, wild type enzyme, in 50 mM acetate buffer pH 5.0, at 23°C | Escherichia coli | |
12000 | - |
H2O2 | catalase activity, wild type enzyme, 100 mM phosphate buffer pH 7.0, at 23°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.7 | - |
H2O2 | catalase activity, mutant enzyme KatGDELTAFG, 100 mM phosphate buffer pH 7.0, at 23°C | Escherichia coli | |
3100 | - |
H2O2 | catalase activity, wild type enzyme, 100 mM phosphate buffer pH 7.0, at 23°C | Escherichia coli |