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Literature summary for 1.11.1.21 extracted from

  • Li, Y.; Goodwin, D.C.
    Vital roles of an interhelical insertion in catalase-peroxidase bifunctionality (2004), Biochem. Biophys. Res. Commun., 318, 970-976.
    View publication on PubMed
Search for more literature for 1.11.1.21

Cloned(Commentary)

Cloned (Comment) Organism
mutant KatGDELTAFG is expressed in Escherichia coli BL-21 (DE3) pLysS cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information the heme environment of mutant KatGDELTAFG (lacking its FG insertion) is highly similar to wild type KatG, but the variant retains only 0.2% catalase activity. In contrast, the deletion reduces peroxidase activity by only 50% Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme KatGDELTAFG, in 50 mM acetate buffer pH 5.0, at 23°C Escherichia coli
0.23
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, wild type enzyme, in 50 mM acetate buffer pH 5.0, at 23°C Escherichia coli
0.83
-
 H2O2 peroxidase activity, wild type enzyme, in 50 mM acetate buffer pH 5.0, at 23°C Escherichia coli
1.5
-
 H2O2 peroxidase activity, mutant enzyme KatGDELTAFG, in 50 mM acetate buffer pH 5.0, at 23°C Escherichia coli
4
-
 H2O2 catalase activity, wild type enzyme, 100 mM phosphate buffer pH 7.0, at 23°C Escherichia coli
4.5
-
 H2O2 catalase activity, mutant enzyme KatGDELTAFG, 100 mM phosphate buffer pH 7.0, at 23°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Escherichia coli oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?
H2O2
-
 Escherichia coli O2 + H2O
-
 ?

Synonyms

Synonyms Comment Organism
KatG
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
26
-
 H2O2 catalase activity, mutant enzyme KatGDELTAFG, 100 mM phosphate buffer pH 7.0, at 23°C Escherichia coli
26
-
 H2O2 peroxidase activity, mutant enzyme KatGDELTAFG, in 50 mM acetate buffer pH 5.0, at 23°C Escherichia coli
26
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme KatGDELTAFG, in 50 mM acetate buffer pH 5.0, at 23°C Escherichia coli
52
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, wild type enzyme, in 50 mM acetate buffer pH 5.0, at 23°C Escherichia coli
58
-
 H2O2 peroxidase activity, wild type enzyme, in 50 mM acetate buffer pH 5.0, at 23°C Escherichia coli
12000
-
 H2O2 catalase activity, wild type enzyme, 100 mM phosphate buffer pH 7.0, at 23°C Escherichia coli

Cofactor

Cofactor Comment Organism Structure
heme
-
 Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.7
-
 H2O2 catalase activity, mutant enzyme KatGDELTAFG, 100 mM phosphate buffer pH 7.0, at 23°C Escherichia coli
3100
-
 H2O2 catalase activity, wild type enzyme, 100 mM phosphate buffer pH 7.0, at 23°C Escherichia coli