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Literature summary for 1.11.1.21 extracted from
- Characterization of a bifunctional catalase-peroxidase of Burkholderia cenocepacia (2007), FEMS Immunol. Med. Microbiol., 50, 37-44.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 Star One Shot cells | Burkholderia cenocepacia |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the peroxidase activity of the enzyme against 3,3',5,5'-tetramethylbenzidine, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), and o-dianisidine is unaffected by the inhibitors isonicotinic acid and niacinamide (all 20 mM), the enzyme is not inhibited by the specific monofunctional catalase inhibitor 3-amino-1,2,4,-triazole | Burkholderia cenocepacia |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
x * 80000, recombinant enzyme, SDS-PAGE | Burkholderia cenocepacia |
| 80500 | - |
x * 80500, calculated from amino acid sequence | Burkholderia cenocepacia |
| 97000 | - |
x * 97000, mature catalase-peroxidase, SDS-PAGE | Burkholderia cenocepacia |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia cenocepacia | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Burkholderia cenocepacia | oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? |  |
| 3,3',5,5'-tetramethylbenzidine + H2O2 | - |
Burkholderia cenocepacia | oxidized 3,3',5,5'-tetramethylbenzidine + H2O | - |
? | |
| H2O2 | - |
Burkholderia cenocepacia | O2 + H2O | - |
? | |
| additional information | guaicol (methoxyphenol) and 2-chloronaphthol are not peroxidized by the enzyme | Burkholderia cenocepacia | ? | - |
? | |
| o-dianisidine + H2O2 | - |
Burkholderia cenocepacia | oxidized o-dianisidine + H2O | - |
? | |
| pyrogallol + H2O2 | - |
Burkholderia cenocepacia | ? + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 80000, recombinant enzyme, SDS-PAGE | Burkholderia cenocepacia |
| ? | x * 80500, calculated from amino acid sequence | Burkholderia cenocepacia |
| ? | x * 97000, mature catalase-peroxidase, SDS-PAGE | Burkholderia cenocepacia |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| KatG | - |
Burkholderia cenocepacia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
pH optimum for peroxidase activity | Burkholderia cenocepacia |
| 6 | - |
pH optimum for catalase activity | Burkholderia cenocepacia |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.5 | pH range for both catalase and peroxidase activities, little or no activity is seen at pH 9.0 | Burkholderia cenocepacia |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Burkholderia cenocepacia | |
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Release 2023.1 (January 2023)
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