Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Synechocystis sp. |
Protein Variants | Comment | Organism |
---|---|---|
D152N | site-directed mutagenesis, 2.7% remaining catalase activity and 2-7times higher peroxidase activity compared to the wild-type enzyme, highly altered pH profile | Synechocystis sp. |
D152S | site-directed mutagenesis, 5.7% remaining catalase activity and 2-7times higher peroxidase activity compared to the wild-type enzyme, highly altered pH profile | Synechocystis sp. |
D152W | site-directed mutagenesis, 0.6% remaining catalase activity and 2-7times higher peroxidase activity compared to the wild-type enzyme, highly altered pH profile | Synechocystis sp. |
P151A | site-directed mutagenesis, slightly increased catalase activity compared to the wild-type enzyme | Synechocystis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady- and transient-state kinetics | Synechocystis sp. | |
2.4 | - |
H2O2 | mutant D152S, pH 7.0, 30°C | Synechocystis sp. | |
2.5 | - |
H2O2 | mutants D152N and P151A, pH 7.0, 30°C | Synechocystis sp. | |
3.2 | - |
H2O2 | mutant D152W, pH 7.0, 30°C | Synechocystis sp. | |
4.1 | - |
H2O2 | wild-type enzyme, pH 7.0, 30°C | Synechocystis sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | heme | Synechocystis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | Synechocystis sp. | - |
O2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
strain PCC 6803 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 H2O2 = O2 + 2 H2O | distal site Asp152 is essential for the catalase activity of catalase-peroxidase, mechanism for hydrogen peroxide oxidation in volving residues Trp122 and Asp152 | Synechocystis sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
substrate guajacol, mutant P151A | Synechocystis sp. |
0.6 | - |
substrate guajacol, wild-type enzyme | Synechocystis sp. |
2.4 | - |
substrate o-dianisidine, mutant P151A | Synechocystis sp. |
2.5 | - |
substrate guajacol, mutants D152N and D152S | Synechocystis sp. |
3.2 | - |
substrate o-dianisidine, wild-type enzyme | Synechocystis sp. |
4.1 | - |
substrate guajacol, mutant D152W | Synechocystis sp. |
4.1 | - |
substrate pyrogallol, mutant P151A | Synechocystis sp. |
6.6 | - |
substrate pyrogallol, wild-type enzyme | Synechocystis sp. |
7.3 | - |
substrate pyrogallol, mutant D152W | Synechocystis sp. |
7.5 | - |
substrate o-dianisidine, mutant D152N | Synechocystis sp. |
7.6 | - |
substrate o-dianisidine, mutant D152S | Synechocystis sp. |
12.5 | - |
substrate pyrogallol, mutant D152N | Synechocystis sp. |
13.6 | - |
substrate pyrogallol, mutant D152S | Synechocystis sp. |
21.5 | - |
substrate o-dianisidine, mutant D152W | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guajacol + H2O2 | peroxidase activity | Synechocystis sp. | ? + H2O | - |
? | |
H2O2 | - |
Synechocystis sp. | O2 + H2O | - |
? | |
H2O2 | catalase activity | Synechocystis sp. | O2 + H2O | - |
? | |
additional information | bifunctional enzyme providing catalase and peroxidase activities, intermediate structure | Synechocystis sp. | ? | - |
? | |
o-dianisidine + H2O2 | peroxidase activity | Synechocystis sp. | ? + H2O | - |
? | |
pyrogallol + H2O2 | peroxidase activity | Synechocystis sp. | ? + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
catalase-peroxidase | - |
Synechocystis sp. |
KatG | - |
Synechocystis sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Synechocystis sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20 | - |
H2O2 | mutant D152W, pH 7.0, 30°C | Synechocystis sp. | |
95 | - |
H2O2 | mutant D152N, pH 7.0, 30°C | Synechocystis sp. | |
200 | - |
H2O2 | mutant D152S, pH 7.0, 30°C | Synechocystis sp. | |
2500 | - |
H2O2 | mutant P151A, pH 7.0, 30°C | Synechocystis sp. | |
3500 | - |
H2O2 | wild-type enzyme, pH 7.0, 30°C | Synechocystis sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Synechocystis sp. |