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Literature summary for 1.11.1.21 extracted from
- Isoniazid activation defects in recombinant Mycobacterium tuberculosis catalase-peroxidase (KatG) mutants evident in InhA inhibitor production (2003), Antimicrob. Agents Chemother., 47, 670-675.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant enzymes in enzyme-deficient Escherichia coli strain UM262 | Mycobacterium tuberculosis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A110V | site-directed mutagenesis, catalytic efficiency with and activation of isoniazid similar to the wild-type enzyme, reduced Km and increased kcat compared to the wild-type | Mycobacterium tuberculosis |
| A139P | site-directed mutagenesis, 76% decreased activity with and activation of isoniazid compared to the wild-type enzyme | Mycobacterium tuberculosis |
| D735A | site-directed mutagenesis, 73% decreased activity with and activation of isoniazid compared to the wild-type enzyme | Mycobacterium tuberculosis |
| L619P | site-directed mutagenesis, no remaining activity with and activation of isoniazid | Mycobacterium tuberculosis |
| L634F | site-directed mutagenesis, 36% decreased activity with and activation of isoniazid compared to the wild-type enzyme | Mycobacterium tuberculosis |
| S315N | site-directed mutagenesis, no remaining activity with and activation of isoniazid | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.063 | - |
2-dianisidine | recombinant mutant D735A, 23°C | Mycobacterium tuberculosis |  |
| 0.064 | - |
2-dianisidine | recombinant mutant A110V, 23°C | Mycobacterium tuberculosis | |
| 0.065 | - |
2-dianisidine | recombinant mutant L634F, 23°C | Mycobacterium tuberculosis | |
| 0.078 | - |
2-dianisidine | recombinant mutant L619P, 23°C | Mycobacterium tuberculosis | |
| 0.081 | - |
2-dianisidine | recombinant mutant S315N, 23°C | Mycobacterium tuberculosis | |
| 0.084 | - |
2-dianisidine | recombinant wild-type enzyme, 23°C | Mycobacterium tuberculosis | |
| 0.096 | - |
2-dianisidine | recombinant mutant A139P, 23°C | Mycobacterium tuberculosis | |
| 5.9 | - |
H2O2 | recombinant wild-type enzyme, 23°C | Mycobacterium tuberculosis | |
| 10.3 | - |
H2O2 | recombinant mutant A110V, 23°C | Mycobacterium tuberculosis | |
| 10.7 | - |
H2O2 | recombinant mutant A139P, 23°C | Mycobacterium tuberculosis | |
| 14.3 | - |
H2O2 | recombinant mutant L634F, 23°C | Mycobacterium tuberculosis | |
| 14.8 | - |
H2O2 | recombinant mutant D735A, 23°C | Mycobacterium tuberculosis | |
| 16.8 | - |
H2O2 | recombinant mutant S315N, 23°C | Mycobacterium tuberculosis | |
| 17.1 | - |
H2O2 | recombinant mutant L619P, 23°C | Mycobacterium tuberculosis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | activates isoniazid activation, not essential | Mycobacterium tuberculosis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2O2 | Mycobacterium tuberculosis | - |
O2 + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
enzyme KatG, gene katG | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant enzymes from Escherichia coli strain UM262, 50-90% homogeneity | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
isoniazid activation activities of wild-type and mutant enzymes in absence or presence of Mn2+, overview | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-dianisidine + tert-butyl hydroperoxide | peroxidase activity | Mycobacterium tuberculosis | ? | - |
? | |
| H2O2 | - |
Mycobacterium tuberculosis | O2 + H2O | - |
? | |
| H2O2 | catalase activity | Mycobacterium tuberculosis | O2 + H2O | - |
? | |
| isoniazid + NADH | isoniazid activation | Mycobacterium tuberculosis | isonicotinic acyl-NADH | the product is a strong inhibitor of the NADH-dependent enoyl-[acyl carrier protein] reductase InhA and the beta-ketoacyl [acyl carrier protein] synthase | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| catalase-peroxidase | - |
Mycobacterium tuberculosis |
| KatG | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.86 | - |
2-dianisidine | recombinant mutant S315N, 23°C | Mycobacterium tuberculosis | |
| 0.99 | - |
2-dianisidine | recombinant mutants L634F and D735A, 23°C | Mycobacterium tuberculosis | |
| 1.06 | - |
2-dianisidine | recombinant mutant L619P, 23°C | Mycobacterium tuberculosis | |
| 1.69 | - |
2-dianisidine | recombinant mutant A139P, 23°C | Mycobacterium tuberculosis | |
| 3.48 | - |
2-dianisidine | recombinant wild-type enzyme, 23°C | Mycobacterium tuberculosis | |
| 4.85 | - |
2-dianisidine | recombinant mutant A110V, 23°C | Mycobacterium tuberculosis | |
| 2750 | - |
H2O2 | recombinant mutant D735A, 23°C | Mycobacterium tuberculosis | |
| 2990 | - |
H2O2 | recombinant mutant S315N, 23°C | Mycobacterium tuberculosis | |
| 3180 | - |
H2O2 | recombinant mutant L619P, 23°C | Mycobacterium tuberculosis | |
| 3270 | - |
H2O2 | recombinant wild-type enzyme, 23°C | Mycobacterium tuberculosis | |
| 3330 | - |
H2O2 | recombinant mutant A139P, 23°C | Mycobacterium tuberculosis | |
| 3450 | - |
H2O2 | recombinant mutant L634F, 23°C | Mycobacterium tuberculosis | |
| 5230 | - |
H2O2 | recombinant mutant A110V, 23°C | Mycobacterium tuberculosis | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | 1.2-1.5 hemes per enzyme dimer | Mycobacterium tuberculosis | |
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