Literature summary for 1.11.1.19 extracted from

Rai, A.; Klare, J.P.; Reinke, P.Y.A.; Englmaier, F.; Fohrer, J.; Fedorov, R.; Taft, M.H.; Chizhov, I.; Curth, U.; Plettenburg, O.; Manstein, D.J.
Structural and biochemical characterization of a dye-decolorizing peroxidase from Dictyostelium discoideum (2021), Int. J. Mol. Sci., 22, 6265 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta(DE3)pLysS cells	Dictyostelium discoideum

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme in the absence of substrate as well as in the presence of potassium cyanide and veratryl alcohol, hanging drop vapor diffusion method, using 2.4 M sodium malonate pH 7.0	Dictyostelium discoideum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.04	-	Reactive Blue 4	at pH 3.0 and 25°C	Dictyostelium discoideum
0.166	-	veratryl alcohol	at pH 4.0 and 25°C	Dictyostelium discoideum
0.37	-	H2O2	at pH 4.0 and 25°C	Dictyostelium discoideum
4.1	-	2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)	at pH 4.0 and 25°C	Dictyostelium discoideum
119	-	pyrogallol	at pH 4.0 and 25°C	Dictyostelium discoideum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Dictyostelium discoideum	Q556V8	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+	-	Dictyostelium discoideum	oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O	-	?
guaiacylglycerol-beta-guaiacyl ether + H2O2 + H+	-	Dictyostelium discoideum	oxidized guaiacylglycerol-beta-guaiacyl ether + H2O	-	?
pyrogallol + H2O2 + H+	-	Dictyostelium discoideum	oxidized pyrogallol + H2O	-	?
Reactive Blue 4 + H2O2 + H+	-	Dictyostelium discoideum	oxidized Reactive Blue 4 + H2O	-	?
veratryl alcohol + H2O2 + H+	-	Dictyostelium discoideum	veratraldehyde + H2O	-	?

Subunits

Subunits	Comment	Organism
homodimer	-	Dictyostelium discoideum

Synonyms

Synonyms	Comment	Organism
dye-decolorizing peroxidase	-	Dictyostelium discoideum
DyPA	-	Dictyostelium discoideum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
20	40	the enzyme remains stable from 20-40°C	Dictyostelium discoideum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.000338	-	veratryl alcohol	at pH 4.0 and 25°C	Dictyostelium discoideum
5.2	-	Reactive Blue 4	at pH 3.0 and 25°C	Dictyostelium discoideum
7.9	-	pyrogallol	at pH 4.0 and 25°C	Dictyostelium discoideum
89.9	-	2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)	at pH 4.0 and 25°C	Dictyostelium discoideum
93.65	-	H2O2	at pH 4.0 and 25°C	Dictyostelium discoideum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3	-	optimum pH for oxidation of Reactive Blue 4	Dictyostelium discoideum
4	-	optimum pH for oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), pyrogallol and veratryl alcohol	Dictyostelium discoideum

Cofactor

Cofactor	Comment	Organism	Structure
heme	-	Dictyostelium discoideum

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.00203	-	veratryl alcohol	at pH 4.0 and 25°C	Dictyostelium discoideum
0.06638	-	pyrogallol	at pH 4.0 and 25°C	Dictyostelium discoideum
21.9	-	2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)	at pH 4.0 and 25°C	Dictyostelium discoideum
130	-	Reactive Blue 4	at pH 3.0 and 25°C	Dictyostelium discoideum
253	-	H2O2	at pH 4.0 and 25°C	Dictyostelium discoideum

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Release 2023.1 (January 2023)