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Literature summary for 1.11.1.19 extracted from

  • Zitare, U.A.; Habib, M.H.; Rozeboom, H.; Mascotti, M.L.; Todorovic, S.; Fraaije, M.W.
    Mutational and structural analysis of an ancestral fungal dye-decolorizing peroxidase (2021), FEBS J., 288, 3602-3618 .
    View publication on PubMed
Search for more literature for 1.11.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 AI or NEB 10beta cells Basidiomycota sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 1.6-1.95 M ammonium sulfate and 0.1 M MES or 0.1 M citrate buffer pH 6.5 Basidiomycota sp.

Protein Variants

Protein Variants Comment Organism
D222A the mutant shows reduced catalytic efficiency with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), Reactive Black 5 and H2O, as compared to the wild type enzyme Basidiomycota sp.
D222E the mutant shows increased catalytic efficiency with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and reduced activity with Reactive Black 5 and H2O, as compared to the wild type enzyme Basidiomycota sp.
D222E/R390K the mutant shows increased catalytic efficiency with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and reduced activity with Reactive Black 5 and H2O, as compared to the wild type enzyme Basidiomycota sp.
R390A the mutant shows reduced catalytic efficiency with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), Reactive Black 5 and H2O, as compared to the wild type enzyme Basidiomycota sp.
R390K the mutant shows increased catalytic efficiency with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and H2O and reduced activity with Reactive Black 5, as compared to the wild type enzyme Basidiomycota sp.

Inhibitors

Inhibitors Comment Organism Structure
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
 Basidiomycota sp.
Direct blue 71
-
 Basidiomycota sp.
Reactive Blue 19
-
 Basidiomycota sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00023
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme D222A, at pH 3.0 and 25°C Basidiomycota sp.
0.0029
-
 Direct Red 80 wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
0.0033
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme D222E, at pH 3.0 and 25°C Basidiomycota sp.
0.0042
-
 Reactive Black 5 mutant enzyme D222A, at pH 3.0 and 25°C Basidiomycota sp.
0.006
-
 Reactive Black 5 mutant enzyme R390A, at pH 3.0 and 25°C Basidiomycota sp.
0.0068
-
 Reactive Red 2 wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
0.0089
-
 Reactive Black 5 mutant enzyme D222E, at pH 3.0 and 25°C Basidiomycota sp.
0.01
-
 Reactive Black 5 mutant enzyme D222E/R390K, at pH 3.0 and 25°C Basidiomycota sp.
0.011
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme R390A, at pH 3.0 and 25°C Basidiomycota sp.
0.013
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme R390K, at pH 3.0 and 25°C Basidiomycota sp.
0.013
-
 Reactive Black 5 wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
0.013
-
 Reactive Black 5 mutant enzyme R390K, at pH 3.0 and 25°C Basidiomycota sp.
0.013
-
 Direct blue 71 wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
0.015
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme D222E/R390K, at pH 3.0 and 25°C Basidiomycota sp.
0.018
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
0.031
-
 H2O2 mutant enzyme R390K, at pH 3.0 and 25°C Basidiomycota sp.
0.039
-
 H2O2 wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
0.042
-
 Reactive Blue 19 wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
0.061
-
 H2O2 mutant enzyme D222E/R390K, at pH 3.0 and 25°C Basidiomycota sp.
0.095
-
 H2O2 mutant enzyme D222E, at pH 3.0 and 25°C Basidiomycota sp.
1.93
-
 H2O2 mutant enzyme D222A, at pH 3.0 and 25°C Basidiomycota sp.
4.18
-
 H2O2 mutant enzyme R390A, at pH 3.0 and 25°C Basidiomycota sp.
80
-
 Mn2+ wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.

Organism

Organism UniProt Comment Textmining
Basidiomycota sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography and Superdex 75 gel filtration Basidiomycota sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + 2 H+
-
 Basidiomycota sp. oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + 2 H2O
-
 ?
Direct Blue 71 + H2O2 + H+
-
 Basidiomycota sp. ?
-
 ?
Direct Red 80 + H2O2 + H+
-
 Basidiomycota sp. ?
-
 ?
Mn2+ + H2O2 + H+
-
 Basidiomycota sp. Mn3+ + 2 H2O
-
 ?
additional information no activity with thioanisole Basidiomycota sp. ?
-
 ?
Reactive Black 5 + H2O2 + H+
-
 Basidiomycota sp. oxidized Reactive Black 5 + H2O
-
 ?
Reactive Blue 19 + H2O2 + H+
-
 Basidiomycota sp. oxidized Reactive Blue 19 + H2O
-
 ?
Reactive Red 2 + H2O2 + H+
-
 Basidiomycota sp. ?
-
 ?

Subunits

Subunits Comment Organism
? x * 69000, calculated from amino acid sequence Basidiomycota sp.
? x * 68992, SUMO-fused enzyme, SDS-PAGE Basidiomycota sp.

Synonyms

Synonyms Comment Organism
AncDyPD-b1
-
 Basidiomycota sp.
DyP
-
 Basidiomycota sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 60% activity loss is observed after 1 h incubation at 40°C Basidiomycota sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.002
-
 Reactive Black 5 mutant enzyme D222A, at pH 3.0 and 25°C Basidiomycota sp.
0.015
-
 Reactive Black 5 mutant enzyme D222E/R390K, at pH 3.0 and 25°C Basidiomycota sp.
0.036
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme D222A, at pH 3.0 and 25°C Basidiomycota sp.
0.038
-
 Reactive Black 5 mutant enzyme D222E, at pH 3.0 and 25°C Basidiomycota sp.
0.04
-
 Direct Red 80 wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
0.11
-
 Reactive Black 5 mutant enzyme R390A, at pH 3.0 and 25°C Basidiomycota sp.
0.12
-
 Reactive Black 5 mutant enzyme R390K, at pH 3.0 and 25°C Basidiomycota sp.
0.14
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme R390A, at pH 3.0 and 25°C Basidiomycota sp.
0.19
-
 Reactive Red 2 wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
0.2
-
 Direct blue 71 wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
0.28
-
 H2O2 mutant enzyme D222A, at pH 3.0 and 25°C Basidiomycota sp.
0.47
-
 H2O2 mutant enzyme R390A, at pH 3.0 and 25°C Basidiomycota sp.
0.5
-
 Mn2+ wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
0.58
-
 Reactive Black 5 wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
1.6
-
 H2O2 mutant enzyme D222E/R390K, at pH 3.0 and 25°C Basidiomycota sp.
2.5
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme D222E, at pH 3.0 and 25°C Basidiomycota sp.
4.3
-
 H2O2 mutant enzyme D222E, at pH 3.0 and 25°C Basidiomycota sp.
5
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme D222E/R390K, at pH 3.0 and 25°C Basidiomycota sp.
5.4
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
5.4
-
 H2O2 wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
7.1
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme R390K, at pH 3.0 and 25°C Basidiomycota sp.
13.6
-
 H2O2 mutant enzyme R390K, at pH 3.0 and 25°C Basidiomycota sp.
22
-
 Reactive Blue 19 wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.

pH Range

pH Minimum pH Maximum Comment Organism
3 4 there is no significant activity at pH 7.0 Basidiomycota sp.

Cofactor

Cofactor Comment Organism Structure
heme
-
 Basidiomycota sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.003
-
 Direct blue 71 wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
0.032
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme D222E/R390K, at pH 3.0 and 25°C Basidiomycota sp.
0.047
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme R390K, at pH 3.0 and 25°C Basidiomycota sp.
0.072
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
0.078
-
 Reactive Blue 19 wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
0.079
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme D222A, at pH 3.0 and 25°C Basidiomycota sp.
0.09
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme D222E, at pH 3.0 and 25°C Basidiomycota sp.
0.19
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme R390A, at pH 3.0 and 25°C Basidiomycota sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0063
-
 Mn2+ wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
0.11
-
 H2O2 mutant enzyme R390A, at pH 3.0 and 25°C Basidiomycota sp.
0.14
-
 H2O2 mutant enzyme D222A, at pH 3.0 and 25°C Basidiomycota sp.
0.45
-
 Reactive Black 5 mutant enzyme D222A, at pH 3.0 and 25°C Basidiomycota sp.
1.5
-
 Reactive Black 5 mutant enzyme D222E/R390K, at pH 3.0 and 25°C Basidiomycota sp.
4.2
-
 Reactive Black 5 mutant enzyme D222E, at pH 3.0 and 25°C Basidiomycota sp.
8.9
-
 Reactive Black 5 mutant enzyme R390K, at pH 3.0 and 25°C Basidiomycota sp.
12
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme R390A, at pH 3.0 and 25°C Basidiomycota sp.
13
-
 Direct Red 80 wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
15
-
 Direct blue 71 wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
18
-
 Reactive Black 5 mutant enzyme R390A, at pH 3.0 and 25°C Basidiomycota sp.
26
-
 H2O2 mutant enzyme D222E/R390K, at pH 3.0 and 25°C Basidiomycota sp.
28
-
 Reactive Red 2 wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
45
-
 H2O2 mutant enzyme D222E, at pH 3.0 and 25°C Basidiomycota sp.
46
-
 Reactive Black 5 wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
140
-
 H2O2 wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
150
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme D222A, at pH 3.0 and 25°C Basidiomycota sp.
290
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
330
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme D222E/R390K, at pH 3.0 and 25°C Basidiomycota sp.
440
-
 H2O2 mutant enzyme R390K, at pH 3.0 and 25°C Basidiomycota sp.
530
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme R390K, at pH 3.0 and 25°C Basidiomycota sp.
530
-
 Reactive Blue 19 wild type enzyme, at pH 3.0 and 25°C Basidiomycota sp.
770
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme D222E, at pH 3.0 and 25°C Basidiomycota sp.