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Literature summary for 1.11.1.19 extracted from

  • Musengi, A.; Durrell, K.; Prins, A.; Khan, N.; Agunbiade, M.; Kudanga, T.; Kirby-McCullough, B.; Pletschke, B.; Burton, S.; Le Roes-Hill, M.
    Production and characterisation of a novel actinobacterial DyP-type peroxidase and its application in coupling of phenolic monomers (2020), Enzyme Microb. Technol., 141, 109654 .
    View publication on PubMed
Search for more literature for 1.11.1.19

Inhibitors

Inhibitors Comment Organism Structure
4-arbutin 24.2% inhibition at 1.0 mM, 66.4% inhibition at 50 mM Streptomyces albidoflavus
acetonitrile less than 5% residual activity at 40% (v/v) Streptomyces albidoflavus
ascorbate 39.5% inhibition at 0.05 mM, complete inhibition at 0.5 mM Streptomyces albidoflavus
azide 26.6% inhibition at 5 mM, 68.4% inhibition at 50 mM Streptomyces albidoflavus
DMSO less than 10% residual activity at 40% (v/v) Streptomyces albidoflavus
ethanol about 30% residual activity at 40% (v/v) Streptomyces albidoflavus
H2O2 42% inhibition at 0.01 mM, complete inhibition at 0.05 mM Streptomyces albidoflavus
L-cysteine 33.9% inhibition at 0.02 mM, complete inhibition at 0.1 mM Streptomyces albidoflavus
Metabisulfite 93.5% inhibition at 0.5 mM, complete inhibition at 1.0 mM Streptomyces albidoflavus
methanol about 40% residual activity at 40% (v/v) Streptomyces albidoflavus
additional information not inhibited by EDTA, SDS, and NaCl Streptomyces albidoflavus
Propanol less than 40% residual activity at 30% (v/v) Streptomyces albidoflavus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.95
-
 2,4-Dichlorophenol at pH 8.0 and 25°C Streptomyces albidoflavus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ about 170% activity at 1 mM Streptomyces albidoflavus
additional information not affected by Ca2+, Co2+, Fe2+, Mg2+, Mn2+, and Zn2+ Streptomyces albidoflavus

Organism

Organism UniProt Comment Textmining
Streptomyces albidoflavus
-
-
-
Streptomyces albidoflavus BSII#1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
acetone precipitation and ultrafiltration Streptomyces albidoflavus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
46.51
-
 after 46.51fold purification, at pH 8.0 and 25°C Streptomyces albidoflavus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + 2 H+
-
 Streptomyces albidoflavus oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + 2 H2O
-
 ?
2,4-dichlorophenol + H2O2 + H+
-
 Streptomyces albidoflavus ?
-
 ?
2,4-dichlorophenol + H2O2 + H+
-
 Streptomyces albidoflavus BSII#1 ?
-
 ?
2,6-dimethoxyphenol + H2O2 + H+
-
 Streptomyces albidoflavus oxidized 2,6-dimethoxyphenol + H2O
-
 ?
2,6-dimethoxyphenol + H2O2 + H+
-
 Streptomyces albidoflavus BSII#1 oxidized 2,6-dimethoxyphenol + H2O
-
 ?
2-aminophenol + H2O2 + H+
-
 Streptomyces albidoflavus ?
-
 ?
Azure B + H2O2 + H+ approximately 30% decolourisation is observed after 24 h (0.001 mM dye) Streptomyces albidoflavus ?
-
 ?
Azure B + H2O2 + H+ approximately 30% decolourisation is observed after 24 h (0.001 mM dye) Streptomyces albidoflavus BSII#1 ?
-
 ?
caffeic acid + H2O2 + H+
-
 Streptomyces albidoflavus ?
-
 ?
catechol + H2O2 + H+
-
 Streptomyces albidoflavus ?
-
 ?
guaiacol + H2O2 + H+
-
 Streptomyces albidoflavus oxidized guaiacol + H2O
-
 ?
L-DOPA + H2O2 + H+
-
 Streptomyces albidoflavus ?
-
 ?
additional information the enzyme also successfully catalyses coupling reactions between several phenolic monomer pairs including catechin-caffeic acid, catechin-catechol, catechin-guaiacol and guaiacol-syringaldazine Streptomyces albidoflavus ?
-
 ?
additional information no activity with 3-hydroxyanthranilic acid, 4-methylcatechol, L-tyrosine, 4-cresol, resorcinol, and syringaldazine Streptomyces albidoflavus ?
-
 ?
phenol + H2O2 + H+
-
 Streptomyces albidoflavus ?
-
 ?
pyrogallol + H2O2 + H+
-
 Streptomyces albidoflavus oxidized pyrogallol + H2O
-
 ?
Reactive Black 5 + H2O2 + H+ approximately 30% decolourisation is observed after 24 h (0.001 mM dye) Streptomyces albidoflavus oxidized Reactive Black 5 + H2O
-
 ?
Reactive Black 5 + H2O2 + H+ approximately 30% decolourisation is observed after 24 h (0.001 mM dye) Streptomyces albidoflavus BSII#1 oxidized Reactive Black 5 + H2O
-
 ?
Reactive Blue 4 + H2O2 + H+ approximately 30% decolourisation is observed after 24 h (0.001 mM dye) Streptomyces albidoflavus oxidized Reactive Blue 4 + H2O
-
 ?
Reactive Blue 4 + H2O2 + H+ approximately 30% decolourisation is observed after 24 h (0.001 mM dye) Streptomyces albidoflavus BSII#1 oxidized Reactive Blue 4 + H2O
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 46000, SDS-PAGE Streptomyces albidoflavus
homodimer 2 * 45390, calculated from amino acid sequence Streptomyces albidoflavus

Synonyms

Synonyms Comment Organism
DyP
-
 Streptomyces albidoflavus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Streptomyces albidoflavus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 80 the enzyme is active over a wide temperature range (from 5 to 80°C) Streptomyces albidoflavus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 80 the enzyme remains stable at temperatures of up to 80°C for 60 min Streptomyces albidoflavus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
 the enzyme has two pH optima at 3.0 and 8.0, depending on substrate Streptomyces albidoflavus
8
-
 the enzyme has two pH optima at 3.0 and 8.0, depending on substrate Streptomyces albidoflavus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 more than 50% activity after 24 h between pH 5.0-9.0, total loss of activity after the enzyme is incubated at pH 3.0-4.0, and more than 90% loss of activity at pH 4.5 Streptomyces albidoflavus

Cofactor

Cofactor Comment Organism Structure
heme
-
 Streptomyces albidoflavus

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces albidoflavus calculated from amino acid sequence
-
 6.7