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Literature summary for 1.11.1.19 extracted from

  • Li, L.; Wang, T.; Chen, T.; Huang, W.; Zhang, Y.; Jia, R.; He, C.
    Revealing two important tryptophan residues with completely different roles in a dye-decolorizing peroxidase from Irpex lacteus F17 (2021), Biotechnol. Biofuels, 14, 128 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta cells Irpex lacteus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.2 M ammonium acetate, 0.1 M sodium acetate trihydrate and 34% (w/v) PEG 4000 Irpex lacteus

Protein Variants

Protein Variants Comment Organism
W109F the mutant shows decreased oxidative activity and decolorizing ability compared to the wild type enzyme Irpex lacteus
W147F the mutant shows decreased oxidative activity and increased decolorizing ability compared to the wild type enzyme Irpex lacteus
W212F the mutant shows decreased oxidative activity and increased decolorizing ability compared to the wild type enzyme Irpex lacteus
W264D inactive Irpex lacteus
W264E inactive Irpex lacteus
W264F the mutant shows decreased oxidative activity and increased decolorizing ability compared to the wild type enzyme Irpex lacteus
W264G inactive Irpex lacteus
W264H the mutant shows decreased oxidative activity and decolorizing ability compared to the wild type enzyme Irpex lacteus
W264L inactive Irpex lacteus
W264R inactive Irpex lacteus
W264Y the mutant shows decreased oxidative activity and decolorizing ability compared to the wild type enzyme Irpex lacteus
W380F the mutant shows severely decreased oxidative activity and decolorizing ability compared to the wild type enzyme Irpex lacteus
W380G the mutant shows decreased oxidative activity and decolorizing ability compared to the wild type enzyme Irpex lacteus
W380Y the mutation does not weaken the first step of the catalytic cycles (oxidation of the resting state Fe3+ by H2O2) significantly, but it weakens the oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and other reducing substrates which has a larger size than H2O2 Irpex lacteus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00898
-
 2,6-dimethoxyphenol wild type enzyme, at pH 3.5 and 35°C Irpex lacteus
0.01947
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W264R, at pH 3.5 and 35°C Irpex lacteus
0.02008
-
 H2O2 mutant enzyme W380Y, at pH 3.5 and 35°C Irpex lacteus
0.0276
-
 Reactive Blue 19 mutant enzyme W264F, at pH 3.5 and 35°C Irpex lacteus
0.04264
-
 2,6-dimethoxyphenol mutant enzyme W264H, at pH 3.5 and 35°C Irpex lacteus
0.0623
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W264Y, at pH 3.5 and 35°C Irpex lacteus
0.06321
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W147F, at pH 3.5 and 35°C Irpex lacteus
0.06644
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W109F, at pH 3.5 and 35°C Irpex lacteus
0.0665
-
 Reactive Blue 19 mutant enzyme W264H, at pH 3.5 and 35°C Irpex lacteus
0.06729
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 3.5 and 35°C Irpex lacteus
0.0689
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W264H, at pH 3.5 and 35°C Irpex lacteus
0.06919
-
 2,6-dimethoxyphenol mutant enzyme W147F, at pH 3.5 and 35°C Irpex lacteus
0.07148
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W212F, at pH 3.5 and 35°C Irpex lacteus
0.07187
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W264F, at pH 3.5 and 35°C Irpex lacteus
0.07404
-
 Reactive Blue 19 mutant enzyme W212F, at pH 3.5 and 35°C Irpex lacteus
0.08448
-
 H2O2 wild type enzyme, at pH 3.5 and 35°C Irpex lacteus
0.08667
-
 2,6-dimethoxyphenol mutant enzyme W212F, at pH 3.5 and 35°C Irpex lacteus
0.08688
-
 Reactive Blue 19 mutant enzyme W109F, at pH 3.5 and 35°C Irpex lacteus
0.09759
-
 Reactive Blue 19 mutant enzyme W147F, at pH 3.5 and 35°C Irpex lacteus
0.13987
-
 Reactive Blue 19 wild type enzyme, at pH 3.5 and 35°C Irpex lacteus
0.17271
-
 2,6-dimethoxyphenol mutant enzyme W109F, at pH 3.5 and 35°C Irpex lacteus
0.22905
-
 2,6-dimethoxyphenol mutant enzyme W264F, at pH 3.5 and 35°C Irpex lacteus
0.27507
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W380Y, at pH 3.5 and 35°C Irpex lacteus
0.752
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W380F, at pH 3.5 and 35°C Irpex lacteus
0.953
-
 2,6-dimethoxyphenol mutant enzyme W264Y, at pH 3.5 and 35°C Irpex lacteus
1.082
-
 2,6-dimethoxyphenol mutant enzyme W264R, at pH 3.5 and 35°C Irpex lacteus

Organism

Organism UniProt Comment Textmining
Irpex lacteus A0A2P1C6N4
-
-
Irpex lacteus F17 A0A2P1C6N4
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 75 gel filtration Irpex lacteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + 2 H+
-
 Irpex lacteus oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + 2 H2O
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + 2 H+
-
 Irpex lacteus F17 oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + 2 H2O
-
 ?
2,6-dimethoxyphenol + H2O2 + H+
-
 Irpex lacteus oxidized 2,6-dimethoxyphenol + H2O
-
 ?
2,6-dimethoxyphenol + H2O2 + H+
-
 Irpex lacteus F17 oxidized 2,6-dimethoxyphenol + H2O
-
 ?
Direct Sky Blue 5B + H2O2 + H+
-
 Irpex lacteus ?
-
 ?
guaiacol + H2O2 + H+
-
 Irpex lacteus oxidized guaiacol + H2O
-
 ?
guaiacol + H2O2 + H+
-
 Irpex lacteus F17 oxidized guaiacol + H2O
-
 ?
Methyl Orange + H2O2 + H+
-
 Irpex lacteus ?
-
 ?
Reactive Blue 19 + H2O2 + H+
-
 Irpex lacteus oxidized Reactive Blue 19 + H2O
-
 ?
Reactive Blue 4 + H2O2 + H+
-
 Irpex lacteus oxidized Reactive Blue 4 + H2O
-
 ?
Reactive Blue 4 + H2O2 + H+
-
 Irpex lacteus F17 oxidized Reactive Blue 4 + H2O
-
 ?
Reactive Blue 5 + H2O2 + H+
-
 Irpex lacteus oxidized Reactive Blue 5 + H2O
-
 ?
Reactive Blue 5 + H2O2 + H+
-
 Irpex lacteus F17 oxidized Reactive Blue 5 + H2O
-
 ?
Reactive Violet 5 + H2O2 + H+
-
 Irpex lacteus ?
-
 ?

Synonyms

Synonyms Comment Organism
dye-decolorizing peroxidase
-
 Irpex lacteus
DyP4
-
 Irpex lacteus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
18.28
-
 2,6-dimethoxyphenol mutant enzyme W264R, at pH 3.5 and 35°C Irpex lacteus
46.11
-
 Reactive Blue 19 mutant enzyme W264F, at pH 3.5 and 35°C Irpex lacteus
52.78
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W264R, at pH 3.5 and 35°C Irpex lacteus
54.29
-
 2,6-dimethoxyphenol mutant enzyme W264H, at pH 3.5 and 35°C Irpex lacteus
56.25
-
 2,6-dimethoxyphenol mutant enzyme W264Y, at pH 3.5 and 35°C Irpex lacteus
58.99
-
 2,6-dimethoxyphenol wild type enzyme, at pH 3.5 and 35°C Irpex lacteus
63.75
-
 2,6-dimethoxyphenol mutant enzyme W212F, at pH 3.5 and 35°C Irpex lacteus
69.65
-
 2,6-dimethoxyphenol mutant enzyme W109F, at pH 3.5 and 35°C Irpex lacteus
71.21
-
 2,6-dimethoxyphenol mutant enzyme W264F, at pH 3.5 and 35°C Irpex lacteus
72.66
-
 2,6-dimethoxyphenol mutant enzyme W147F, at pH 3.5 and 35°C Irpex lacteus
86.87
-
 Reactive Blue 19 mutant enzyme W264H, at pH 3.5 and 35°C Irpex lacteus
88.38
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W380Y, at pH 3.5 and 35°C Irpex lacteus
108.79
-
 Reactive Blue 19 mutant enzyme W212F, at pH 3.5 and 35°C Irpex lacteus
112.63
-
 Reactive Blue 19 mutant enzyme W109F, at pH 3.5 and 35°C Irpex lacteus
148.99
-
 Reactive Blue 19 mutant enzyme W147F, at pH 3.5 and 35°C Irpex lacteus
165.65
-
 H2O2 mutant enzyme W380Y, at pH 3.5 and 35°C Irpex lacteus
187.9
-
 Reactive Blue 19 wild type enzyme, at pH 3.5 and 35°C Irpex lacteus
243.13
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W264Y, at pH 3.5 and 35°C Irpex lacteus
316.16
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W380F, at pH 3.5 and 35°C Irpex lacteus
384.85
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W264F, at pH 3.5 and 35°C Irpex lacteus
602.5
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W109F, at pH 3.5 and 35°C Irpex lacteus
665.1
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W212F, at pH 3.5 and 35°C Irpex lacteus
684.34
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W264H, at pH 3.5 and 35°C Irpex lacteus
786.7
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W147F, at pH 3.5 and 35°C Irpex lacteus
790
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 3.5 and 35°C Irpex lacteus
1025
-
 H2O2 wild type enzyme, at pH 3.5 and 35°C Irpex lacteus

Cofactor

Cofactor Comment Organism Structure
heme
-
 Irpex lacteus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
16.9
-
 2,6-dimethoxyphenol mutant enzyme W264R, at pH 3.5 and 35°C Irpex lacteus
59
-
 2,6-dimethoxyphenol mutant enzyme W264Y, at pH 3.5 and 35°C Irpex lacteus
311
-
 2,6-dimethoxyphenol mutant enzyme W264F, at pH 3.5 and 35°C Irpex lacteus
321
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W380Y, at pH 3.5 and 35°C Irpex lacteus
403
-
 2,6-dimethoxyphenol mutant enzyme W109F, at pH 3.5 and 35°C Irpex lacteus
420
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W380F, at pH 3.5 and 35°C Irpex lacteus
730
-
 2,6-dimethoxyphenol mutant enzyme W212F, at pH 3.5 and 35°C Irpex lacteus
1050
-
 2,6-dimethoxyphenol mutant enzyme W147F, at pH 3.5 and 35°C Irpex lacteus
1270
-
 2,6-dimethoxyphenol mutant enzyme W264H, at pH 3.5 and 35°C Irpex lacteus
1300
-
 Reactive Blue 19 mutant enzyme W109F, at pH 3.5 and 35°C Irpex lacteus
1310
-
 Reactive Blue 19 mutant enzyme W264H, at pH 3.5 and 35°C Irpex lacteus
1340
-
 Reactive Blue 19 wild type enzyme, at pH 3.5 and 35°C Irpex lacteus
1470
-
 Reactive Blue 19 mutant enzyme W212F, at pH 3.5 and 35°C Irpex lacteus
1530
-
 Reactive Blue 19 mutant enzyme W147F, at pH 3.5 and 35°C Irpex lacteus
1670
-
 Reactive Blue 19 mutant enzyme W264F, at pH 3.5 and 35°C Irpex lacteus
2710
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W264R, at pH 3.5 and 35°C Irpex lacteus
3900
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W264Y, at pH 3.5 and 35°C Irpex lacteus
5350
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W264F, at pH 3.5 and 35°C Irpex lacteus
6570
-
 2,6-dimethoxyphenol wild type enzyme, at pH 3.5 and 35°C Irpex lacteus
8250
-
 H2O2 mutant enzyme W380Y, at pH 3.5 and 35°C Irpex lacteus
9030
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W109F, at pH 3.5 and 35°C Irpex lacteus
9300
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W212F, at pH 3.5 and 35°C Irpex lacteus
9930
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W264H, at pH 3.5 and 35°C Irpex lacteus
11700
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 3.5 and 35°C Irpex lacteus
12100
-
 H2O2 wild type enzyme, at pH 3.5 and 35°C Irpex lacteus
12500
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W147F, at pH 3.5 and 35°C Irpex lacteus