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Literature summary for 1.11.1.19 extracted from

  • Min, K.; Gong, G.; Woo, H.M.; Kim, Y.; Um, Y.
    A dye-decolorizing peroxidase from Bacillus subtilis exhibiting substrate-dependent optimum temperature for dyes and beta-ether lignin dimer (2015), Sci. Rep., 5, 8245 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis KCTC2023
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, and Superdex 200 gel filtration Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.086
-
 with veratryl glycerol-beta-guaiacyl ether, at pH 3.0 and 50°C Bacillus subtilis
0.13
-
 with veratryl alcohol, at pH 3.0 and 50°C Bacillus subtilis
11.55
-
 with Reactive Blue 19, at pH 3.0 and 30°C Bacillus subtilis
17.65
-
 with Reactive Black 5, at pH 3.0 and 30°C Bacillus subtilis
66.8
-
 with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), at pH 3.0 and 50°C Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Bacillus subtilis ?
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Bacillus subtilis KCTC2023 ?
-
 ?
2,6-dimethoxyphenol + H2O2 + H+
-
 Bacillus subtilis ?
-
 ?
2,6-dimethoxyphenol + H2O2 + H+
-
 Bacillus subtilis KCTC2023 ?
-
 ?
Reactive Black 5 + H2O2 + H+
-
 Bacillus subtilis ?
-
 ?
Reactive Black 5 + H2O2 + H+
-
 Bacillus subtilis KCTC2023 ?
-
 ?
Reactive Blue 19 + H2O2 + H+
-
 Bacillus subtilis ?
-
 ?
Reactive Blue 19 + H2O2 + H+
-
 Bacillus subtilis KCTC2023 ?
-
 ?
veratryl alcohol + H2O2 + H+
-
 Bacillus subtilis ?
-
 ?
veratryl alcohol + H2O2 + H+
-
 Bacillus subtilis KCTC2023 ?
-
 ?
veratryl glycerol-beta-guaiacyl ether + H2O2 + H+
-
 Bacillus subtilis veratryl aldehyde + guaiacol + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
dye-decolorizing peroxidase
-
 Bacillus subtilis
DyP
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 optimum temperature for dyes Bacillus subtilis
50
-
 optimum temperature for high redox potential substrates Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
heme
-
 Bacillus subtilis