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Literature summary for 1.11.1.19 extracted from

  • Linde, D.; Coscolin, C.; Liers, C.; Hofrichter, M.; Martinez, A.T.; Ruiz-Duenas, F.J.
    Heterologous expression and physicochemical characterization of a fungal dye-decolorizing peroxidase from Auricularia auricula-judae (2014), Protein Expr. Purif., 103, 28-37 .
    View publication on PubMed
Search for more literature for 1.11.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLyS cells Auricularia auricula-judae

Inhibitors

Inhibitors Comment Organism Structure
H2O2
-
 Auricularia auricula-judae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 Reactive Black 5 wild type enzyme, at pH 3.0 and 30°C Auricularia auricula-judae
0.12
-
 Reactive Blue 19 wild type enzyme, at pH 3.5 and 30°C Auricularia auricula-judae
0.283
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 3.0 and 30°C Auricularia auricula-judae
1.32
-
 2,6-dimethoxyphenol wild type enzyme, at pH 3.0 and 30°C Auricularia auricula-judae

Organism

Organism UniProt Comment Textmining
Auricularia auricula-judae I2DBY1
-
-

Purification (Commentary)

Purification (Comment) Organism
Q Sepharose column chromatography, Mono Q column chromatography, and Mono P column chromatography Auricularia auricula-judae

Renatured (Commentary)

Renatured (Comment) Organism
with oxidized glutathione (1.5 mM), hemin (0.03 mM) and urea (0.8 M) Auricularia auricula-judae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Auricularia auricula-judae ?
-
 ?
2,6-dimethoxyphenol + H2O2 + H+
-
 Auricularia auricula-judae ?
-
 ?
Reactive Black 5 + H2O2 + H+
-
 Auricularia auricula-judae ?
-
 ?
Reactive Blue 19 + H2O2 + H+
-
 Auricularia auricula-judae ?
-
 ?
veratryl alcohol + H2O2 + H+ very weak substrate Auricularia auricula-judae ?
-
 ?

Subunits

Subunits Comment Organism
? x * 47000, calculated from amino acid sequence Auricularia auricula-judae

Synonyms

Synonyms Comment Organism
dye-decolorizing peroxidase
-
 Auricularia auricula-judae
DyP
-
 Auricularia auricula-judae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 65 the wild type enzyme remains stable after 24 h at 30-55°C and shows 50% activity after 24 h at 65.5°C Auricularia auricula-judae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.6
-
 Reactive Black 5 wild type enzyme, at pH 3.0 and 30°C Auricularia auricula-judae
420
-
 2,6-dimethoxyphenol wild type enzyme, at pH 3.0 and 30°C Auricularia auricula-judae
422
-
 Reactive Blue 19 wild type enzyme, at pH 3.5 and 30°C Auricularia auricula-judae
654
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 3.0 and 30°C Auricularia auricula-judae

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 the enzyme shows more than 80% activity after 24 h at pH 4.0-9.0 Auricularia auricula-judae

pI Value

Organism Comment pI Value Maximum pI Value
Auricularia auricula-judae isoelectric focusing
-
 4.4

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.11
-
 veratryl alcohol wild type enzyme, at pH 2.5 and 30°C Auricularia auricula-judae
320
-
 2,6-dimethoxyphenol wild type enzyme, at pH 3.0 and 30°C Auricularia auricula-judae
400
-
 Reactive Black 5 wild type enzyme, at pH 3.0 and 30°C Auricularia auricula-judae
2300
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 3.0 and 30°C Auricularia auricula-judae
3600
-
 Reactive Blue 19 wild type enzyme, at pH 3.5 and 30°C Auricularia auricula-judae