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Literature summary for 1.11.1.19 extracted from

  • Yu, W.; Liu, W.; Huang, H.; Zheng, F.; Wang, X.; Wu, Y.; Li, K.; Xie, X.; Jin, Y.
    Application of a novel alkali-tolerant thermostable DyP-type peroxidase from Saccharomonospora viridis DSM 43017 in biobleaching of eucalyptus kraft pulp (2014), PLoS ONE, 9, e110319 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.19

Application

Application Comment Organism
paper production the enzyme has an accelerated action during the biobleaching of eucalyptus kraft pulp Saccharomonospora viridis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Saccharomonospora viridis

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 84.7% residual activity at 5 mM Saccharomonospora viridis
Fe3+ 35.9% residual activity at 5 mM Saccharomonospora viridis
Li+ 87% residual activity at 5 mM Saccharomonospora viridis
Mn2+ 92.3% residual activity at 5 mM Saccharomonospora viridis
SDS no activity at 5 mM SDS Saccharomonospora viridis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05887
-
 Brilliant Green at pH 7.0 and 70°C Saccharomonospora viridis
0.1872
-
 Reactive Yellow 2 at pH 7.0 and 70°C Saccharomonospora viridis
0.2102
-
 Reactive Blue 19 at pH 7.0 and 70°C Saccharomonospora viridis

Metals/Ions

Metals/Ions Comment Organism Structure
2-mercaptoethanol 847.9% activity at 5 mM Saccharomonospora viridis
Al3+ 127.6% activity at 5 mM Saccharomonospora viridis
Co2+ 116.9% activity at 5 mM Saccharomonospora viridis
additional information not influenced by Zn2+, Ba2+, Mg2+, Fe2+, and EDTA Saccharomonospora viridis

Organism

Organism UniProt Comment Textmining
Saccharomonospora viridis T1SP91
-
-
Saccharomonospora viridis DSM 43017 T1SP91
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography and Superdex G-75 gel filtration Saccharomonospora viridis

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.8
-
 enzyme from lysate supernatant, at pH 7.0 and 70°C Saccharomonospora viridis
17.8
-
 after 22.3fold purification at pH 7.0 and 70°C Saccharomonospora viridis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-dimethoxyphenol + H2O2 + H+ 0.5% activity compared to Brilliant Green Saccharomonospora viridis ?
-
 ?
Azure B + H2O2 + H+ 13.2% activity compared to Brilliant Green Saccharomonospora viridis ?
-
 ?
Brilliant Green + H2O2 + H+ 100% activity Saccharomonospora viridis ?
-
 ?
Brilliant Green + H2O2 + H+ 100% activity Saccharomonospora viridis DSM 43017 ?
-
 ?
Crystal Violet + H2O2 + H+ 33.6% activity compared to Brilliant Green Saccharomonospora viridis ?
-
 ?
Malachite Green + H2O2 + H+ 68.6% activity compared to Brilliant Green Saccharomonospora viridis ?
-
 ?
Reactive Black 5 + H2O2 + H+ 7.8% activity compared to Brilliant Green Saccharomonospora viridis ?
-
 ?
Reactive Black 5 + H2O2 + H+ 7.8% activity compared to Brilliant Green Saccharomonospora viridis DSM 43017 ?
-
 ?
Reactive Blue 19 + H2O2 10.5% activity compared to Brilliant Green Saccharomonospora viridis ?
-
 ?
Reactive Blue 19 + H2O2 10.5% activity compared to Brilliant Green Saccharomonospora viridis DSM 43017 ?
-
 ?
Reactive Green 19 + H2O2 + H+ 10.8% activity compared to Brilliant Green Saccharomonospora viridis ?
-
 ?
Reactive Green 19 + H2O2 + H+ 10.8% activity compared to Brilliant Green Saccharomonospora viridis DSM 43017 ?
-
 ?
Reactive Red 120 + H2O2 + H+ 5.6% activity compared to Brilliant Green Saccharomonospora viridis ?
-
 ?
Reactive Yellow 2 + H2O2 + H+ 39.7% activity compared to Brilliant Green Saccharomonospora viridis ?
-
 ?
Reactive Yellow 2 + H2O2 + H+ 39.7% activity compared to Brilliant Green Saccharomonospora viridis DSM 43017 ?
-
 ?
veratryl alcohol + H2O2 + H+ 0.2% activity compared to Brilliant Green Saccharomonospora viridis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Saccharomonospora viridis
? x * 44500, calculated from amino acid sequence Saccharomonospora viridis

Synonyms

Synonyms Comment Organism
DyP
-
 Saccharomonospora viridis
DyP-type peroxidase
-
 Saccharomonospora viridis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Saccharomonospora viridis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 80 the enzyme retains about 63% of its maximum activity at 50-80°C Saccharomonospora viridis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 the enzyme shows more than 60% activity after incubation at 70°C for 2 h at pH 7.0 Saccharomonospora viridis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Saccharomonospora viridis

pH Range

pH Minimum pH Maximum Comment Organism
4 9 the enzyme shows more than 35% activity at pH 4.0-9.0 Saccharomonospora viridis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 the enzyme shows more than 80% activity after incubation at pH 5.0-10.0 for 1 h at 37°C Saccharomonospora viridis

Cofactor

Cofactor Comment Organism Structure
heme
-
 Saccharomonospora viridis

pI Value

Organism Comment pI Value Maximum pI Value
Saccharomonospora viridis isoelectric focusing
-
 5.2