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Literature summary for 1.11.1.19 extracted from
- Characterization of a purified decolorizing detergent-stable peroxidase from Streptomyces griseosporeus SN9 (2015), Int. J. Biol. Macromol., 73, 253-263 .
General Stability
| General Stability | Organism |
|---|---|
| the enzyme shows high compatibility with EcoVax and Dipex as laundry detergents for 48 h at 40°C. The enzyme is highly stable against perfume and anti-redeposition agents, particularly 50 mM Na2CO3, and of cationic and other detergent compound, retaining 95, 104, 107, and 125% of its initial activity after treatment with 1% perfume, 50 mM hexadecyltrimethylammonium bromide, 1% (w/v) sodium tripolyphosphate, and 50 mM Na2CO3, respectively. | Streptomyces griseosporeus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | about 85% inhibition at 1 mM | Streptomyces griseosporeus |  |
| Cd2+ | complete inhibition at 1 mM | Streptomyces griseosporeus | |
| Co2+ | about 56% inhibition at 1 mM | Streptomyces griseosporeus | |
| Hg2+ | complete inhibition at 1 mM | Streptomyces griseosporeus | |
| potassium cyanide | 13% inhibition at 1 mM | Streptomyces griseosporeus | |
| Sodium azide | 9% inhibition at 1 mM | Streptomyces griseosporeus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.112 | - |
2,4-Dichlorophenol | at pH 8.5 and 25°C | Streptomyces griseosporeus | |
| 0.458 | - |
o-Dianisidine | at pH 8.5 and 25°C | Streptomyces griseosporeus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Streptomyces griseosporeus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | about 116% activity at 1 mM | Streptomyces griseosporeus | |
| Mn2+ | about 139% activity at 1 mM. The addition of 1 mM of Mn2+ also enhances the thermostability of the enzyme | Streptomyces griseosporeus | |
| additional information | Cu2+, Mg2+, Zn2+, and Fe2+ do not exert significant effects on enzyme activity | Streptomyces griseosporeus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces griseosporeus | - |
- |
- |
| Streptomyces griseosporeus SN9 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| OH-40 column chromatography and UNO Q-6 column chromatography | Streptomyces griseosporeus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | about 92% activity compared to 2,4-dichlorophenol | Streptomyces griseosporeus | ? | - |
? | |
| 2,4,6-trichlorophenol + H2O2 + H+ | about 63% activity compared to 2,4-dichlorophenol | Streptomyces griseosporeus | ? | - |
? | |
| 2,4-dichlorophenol + H2O2 + H+ | 100% activity | Streptomyces griseosporeus | ? | - |
? | |
| 2,6-dimethoxyphenol + H2O2 + H+ | - |
Streptomyces griseosporeus | ? | - |
? | |
| 4-chlorophenol + H2O2 + H+ | about 45% activity compared to 2,4-dichlorophenol | Streptomyces griseosporeus | ? | - |
? | |
| Acid Blue 158 + H2O2 + H+ | - |
Streptomyces griseosporeus | ? | - |
? | |
| Cibacet Brilliant Blue BG + H2O2 + H+ | - |
Streptomyces griseosporeus | ? | - |
? | |
| guaiacol + H2O2 + H+ | about 27% activity compared to 2,4-dichlorophenol | Streptomyces griseosporeus | ? | - |
? | |
| additional information | no activity with commercial humic acid | Streptomyces griseosporeus | ? | - |
? | |
| o-dianisidine + H2O2 + H+ | about 76% activity compared to 2,4-dichlorophenol | Streptomyces griseosporeus | ? | - |
? | |
| pyrogallol + H2O2 + H+ | about 75% activity compared to 2,4-dichlorophenol | Streptomyces griseosporeus | ? | - |
? | |
| Remazol Brilliant Blue Reactif + H2O2 + H+ | - |
Streptomyces griseosporeus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 43000, SDS-PAGE | Streptomyces griseosporeus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LiP-SN | - |
Streptomyces griseosporeus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Streptomyces griseosporeus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
the half-life at 60°C is 120 min | Streptomyces griseosporeus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0635 | - |
2,4-Dichlorophenol | at pH 8.5 and 25°C | Streptomyces griseosporeus | |
| 1.17 | - |
o-Dianisidine | at pH 8.5 and 25°C | Streptomyces griseosporeus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Streptomyces griseosporeus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | the enzyme maintains 100% of its original activity at pH values between 7 and 10 after incubation for 48 h at 40°C | Streptomyces griseosporeus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Streptomyces griseosporeus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.567 | - |
2,4-Dichlorophenol | at pH 8.5 and 25°C | Streptomyces griseosporeus | |
| 2.54 | - |
o-Dianisidine | at pH 8.5 and 25°C | Streptomyces griseosporeus | |
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