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Literature summary for 1.11.1.19 extracted from

  • Lauber, C.; Schwarz, T.; Nguyen, Q.K.; Lorenz, P.; Lochnit, G.; Zorn, H.
    Identification, heterologous expression and characterization of a dye-decolorizing peroxidase of Pleurotus sapidus (2017), AMB Express, 7, 164 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Trichoderma reesei Pleurotus sapidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
 Reactive Blue 5 at pH 4.0 and 30°C Pleurotus sapidus
0.099
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 3.8 and 30°C Pleurotus sapidus
0.713
-
 guaiacol at pH 4.5 and 30°C Pleurotus sapidus
1.227
-
 2,6-dimethoxyphenol at pH 4.5 and 30°C Pleurotus sapidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
 gel filtration Pleurotus sapidus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Reactive Blue 5 + H2O2 Pleurotus sapidus
-
 phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O
-
 ?
Reactive Blue 5 + H2O2 Pleurotus sapidus DSM 8266
-
 phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pleurotus sapidus A0A0F7VJ89
-
-
Pleurotus sapidus DSM 8266 A0A0F7VJ89
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Pleurotus sapidus

Purification (Commentary)

Purification (Comment) Organism
phenyl-Sepharose column chromatography and SP Sepharose 200 column chromatography Pleurotus sapidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Pleurotus sapidus ?
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Pleurotus sapidus DSM 8266 ?
-
 ?
2,6-dimethoxyphenol + H2O2 + H+
-
 Pleurotus sapidus ?
-
 ?
2,6-dimethoxyphenol + H2O2 + H+
-
 Pleurotus sapidus DSM 8266 ?
-
 ?
annatto + H2O2 + H+
-
 Pleurotus sapidus ?
-
 ?
annatto + H2O2 + H+
-
 Pleurotus sapidus DSM 8266 ?
-
 ?
beta-carotene + H2O2 + H+
-
 Pleurotus sapidus ?
-
 ?
beta-carotene + H2O2 + H+
-
 Pleurotus sapidus DSM 8266 ?
-
 ?
guaiacol + H2O2 + H+
-
 Pleurotus sapidus ?
-
 ?
additional information the enzyme has an oxidase function in addition to a peroxidase activity Pleurotus sapidus ?
-
 ?
Reactive Blue 5 + H2O2
-
 Pleurotus sapidus phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O
-
 ?
Reactive Blue 5 + H2O2
-
 Pleurotus sapidus DSM 8266 phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 57100, calculated from amino acid sequence Pleurotus sapidus
homodimer 2 * 57600, SDS-PAGE Pleurotus sapidus

Synonyms

Synonyms Comment Organism
dye-decolorizing peroxidase
-
 Pleurotus sapidus
DyP
-
 Pleurotus sapidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
15 70 after 5 min incubation, respectively, the enzyme shows 100% activity between 15 and 25°C, about 85% activity at 30-35°C, about 75% activity at 40-45°C, about 62% activity at 50°C, and less than 30% activity at 55°C and above Pleurotus sapidus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
18
-
 Reactive Blue 5 at pH 4.0 and 30°C Pleurotus sapidus
60
-
 2,6-dimethoxyphenol at pH 4.5 and 30°C Pleurotus sapidus
74
-
 guaiacol at pH 4.5 and 30°C Pleurotus sapidus
375
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 3.8 and 30°C Pleurotus sapidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5 4.5
-
 Pleurotus sapidus

pI Value

Organism Comment pI Value Maximum pI Value
Pleurotus sapidus calculated from amino acid sequence
-
 5.7
Pleurotus sapidus isoelectric focusing
-
 6.7

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
49
-
 2,6-dimethoxyphenol at pH 4.5 and 30°C Pleurotus sapidus
100
-
 guaiacol at pH 4.5 and 30°C Pleurotus sapidus
750
-
 Reactive Blue 5 at pH 4.0 and 30°C Pleurotus sapidus
3800
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 3.8 and 30°C Pleurotus sapidus